Farah Abid1,2, Muhammad Saleem3, Christian D Muller4, Mulazim Hussain Asim5, Shumaila Arshad2, Tahir Maqbool6, Faheem Hadi6. 1. Department of Pharmacy, Government College University of Faisalabad, Faisalabad, Pakistan. 2. Faculty of Pharmacy, University of Lahore, Defence Road, Lahore, Pakistan. 3. University College of Pharmacy, University of the Punjab, Lahore, Pakistan. 4. Institut Pluridisciplinaire Hubert Curien, UMR 7178 CNRS, Faculté de Pharmacie, Université de Strasbourg, 67401 Illkirch, France. 5. College of Pharmacy, University of Sargodha, Sargodha, Pakistan. 6. Centre of Research in Molecular Medicine, Institute of Molecular Biology & Biotechnology, University of Lahore, Defence Road, Lahore, Pakistan.
Abstract
BACKGROUND: Cancer is one of the leading causes of death in the world. Numerous phytochemicals from plants have shown antineoplastic effects via programmed cell death (apoptosis). The aim of this study was to evaluate the effect of anti-proliferative and apoptosis-inducing activity of Acacia modesta and Opuntia monocantha against HeLa cells. METHODS: To estimate anti-proliferative activity of the plants against HeLa cells, ethanol solvent was used for the extraction. For the evaluation of anti-proliferative effects, MTT assay was performed with 100, 200, and 400 µg/mL dose. The antioxidant assays including glutathione reductase (GSH), superoxide dismutase (SOD) and catalase were performed. Moreover, enzyme linked immunosorbent assay (ELISA) was performed. Furthermore, immunocytometry P53 and flow cytometry were also carried out to assess the apoptosis in HeLa cell. RESULTS: MTT assay showed that the groups treated with Opuntia monocantha and Acacia modest have less level of toxicity as compared to untreated groups. Antioxidant assays confirmed that GSH, SPD and, catalase activities were quite decreased in treated groups as compared to untreated groups. Similarly, ELISA and apoptosis p53 have shown more pronounced apoptosis effect in treated groups as compared to untreated groups. CONCLUSION: Based on above findings, treatment of HeLa cells with these plant extracts induced apoptosis, restricts proliferation, and enhances the anti-oxidative index in post treated cells.<br />.
BACKGROUND:Cancer is one of the leading causes of death in the world. Numerous phytochemicals from plants have shown antineoplastic effects via programmed cell death (apoptosis). The aim of this study was to evaluate the effect of anti-proliferative and apoptosis-inducing activity of Acacia modesta and Opuntiamonocantha against HeLa cells. METHODS: To estimate anti-proliferative activity of the plants against HeLa cells, ethanol solvent was used for the extraction. For the evaluation of anti-proliferative effects, MTT assay was performed with 100, 200, and 400 µg/mL dose. The antioxidant assays including glutathione reductase (GSH), superoxide dismutase (SOD) and catalase were performed. Moreover, enzyme linked immunosorbent assay (ELISA) was performed. Furthermore, immunocytometry P53 and flow cytometry were also carried out to assess the apoptosis in HeLa cell. RESULTS:MTT assay showed that the groups treated with Opuntiamonocantha and Acacia modest have less level of toxicity as compared to untreated groups. Antioxidant assays confirmed that GSH, SPD and, catalase activities were quite decreased in treated groups as compared to untreated groups. Similarly, ELISA and apoptosis p53 have shown more pronounced apoptosis effect in treated groups as compared to untreated groups. CONCLUSION: Based on above findings, treatment of HeLa cells with these plant extracts induced apoptosis, restricts proliferation, and enhances the anti-oxidative index in post treated cells.<br />.