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Literature DB >> 3309162

Cloning and expression of treponema pallidum common antigen (Tp-4) in Escherichia coli K12.

P Hindersson¹, J D Knudsen, N H Axelsen.

Abstract

A library of Treponema pallidum DNA was constructed using a cosmid cloning system. Sixteen hundred Escherichia coli recombinant clones were generated covering the T. pallidum genome with a probability of 99%. Three hundred of the clones were screened for expression of T. pallidum antigens by a modified rocket immunoelectrophoresis technique using a polyspecific antiserum to T. pallidum. One clone was identified which produced the 'common antigen' (CA) of T. pallidum (Tp-4). CA shares epitopes with antigens present in more than 50 different bacterial species, but nothing is known about its structure, function and localization. The recombinant E. coli clone will be of value for a structural analysis of the CA gene.

Entities: Species

Mesh：

Substances：
Antigens, Bacterial

Year: 1987 PMID： 3309162 DOI： 10.1099/00221287-133-3-587

Source DB: PubMed Journal: J Gen Microbiol ISSN： 0022-1287

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Cited

20 in total

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3. Purified 60-kilodalton Legionella protein antigen with Legionella-specific and nonspecific epitopes.

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4. Immunological response to the Brucella abortus GroEL homolog.

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5. Measurement of antibodies to the Borrelia burgdorferi flagellum improves serodiagnosis in Lyme disease.

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6. Antigenic relatedness and N-terminal sequence homology define two classes of periplasmic flagellar proteins of Treponema pallidum subsp. pallidum and Treponema phagedenis.

Authors: S J Norris; N W Charon; R G Cook; M D Fuentes; R J Limberger
Journal: J Bacteriol Date: 1988-09 Impact factor: 3.490

Cloning and expression of treponema pallidum common antigen (Tp-4) in Escherichia coli K12.

1. Molecular cloning and characterization of DNA from human intestinal spirochetes.

Review 2. Stress proteins and the immune response to mycobacteria--antigens as virulence factors?

3. Purified 60-kilodalton Legionella protein antigen with Legionella-specific and nonspecific epitopes.

4. Immunological response to the Brucella abortus GroEL homolog.

5. Measurement of antibodies to the Borrelia burgdorferi flagellum improves serodiagnosis in Lyme disease.

6. Antigenic relatedness and N-terminal sequence homology define two classes of periplasmic flagellar proteins of Treponema pallidum subsp. pallidum and Treponema phagedenis.

7. Bacterial heat shock proteins directly induce cytokine mRNA and interleukin-1 secretion in macrophage cultures.

Review 8. Role of heat shock proteins in protection from and pathogenesis of infectious diseases.

9. Physical map of the genome of Treponema pallidum subsp. pallidum (Nichols).

Review 10. Heat-shock proteins and autoimmunity in humans.