Liping Li1,2, Yuanyuan Liu3,4, Xia Zhao5, Chao Qi5, Yi Zhang5, Yingze Zhang5,6, Tengbo Yu5. 1. Department of Orthopaedic Surgery, The Affiliated Central Hospital of Qingdao University, Qingdao, China. 2. Department of Orthopaedic Surgery, The Second Clinical Medical College of Qingdao University, Qingdao, China. 3. Department of Oncology, The Affiliated Central Hospital of Qingdao University, Qingdao, China. 4. Department of Oncology, The Second Clinical Medical College of Qingdao University, Qingdao, China. 5. Department of Orthopaedic Surgery, Affiliated Hospital of Qingdao University, Qingdao, China. 6. Department of Orthopaedic Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, China.
Abstract
OBJECTIVE: To clarify the effects on and the mechanism of salvianic acid A sodium (SAAS) in the recovery of motor function after spinal cord injury. METHODS: In vivo and in vitro experiments were carried out in this research to determine the effects of SAAS on tissue damage, neuron survival, microglia polarization, and inflammation after spinal cord injury (SCI). Differentially expressed genes treated with SAAS were screened by transcriptome sequencing, and the molecular mechanism was investigated simultaneously. RESULTS: The results revealed that SAAS could promote type M2 polarization of microglia and reduce the proportion of type M1. In this way, it reduced the secretion and expression of inflammatory factors. Compared with Lipopolysaccharides(LPS), 345 genes were upregulated and 407 genes were downregulated in the LPS + SAAS treatment group. In the SAAS group, expression levels of Ndufa12, IL-6, TNF-α, and Vdac1 were significantly reduced, while a marked elevation was found in MIP2. In addition, results found in an animal model showed that SAAS could obviously facilitate motor function recovery of mice after spinal cord injury, and it had a good protective effect on spinal cord tissue and neuron cells. CONCLUSION: As a result, the present study clarified both the protective effect of SAAS on neurons after spinal cord injury and the anti-inflammatory effect of microglia, which is expected to serve as a theoretical basis for clinical treatment.
OBJECTIVE: To clarify the effects on and the mechanism of salvianic acid A sodium (SAAS) in the recovery of motor function after spinal cord injury. METHODS: In vivo and in vitro experiments were carried out in this research to determine the effects of SAAS on tissue damage, neuron survival, microglia polarization, and inflammation after spinal cord injury (SCI). Differentially expressed genes treated with SAAS were screened by transcriptome sequencing, and the molecular mechanism was investigated simultaneously. RESULTS: The results revealed that SAAS could promote type M2 polarization of microglia and reduce the proportion of type M1. In this way, it reduced the secretion and expression of inflammatory factors. Compared with Lipopolysaccharides(LPS), 345 genes were upregulated and 407 genes were downregulated in the LPS + SAAS treatment group. In the SAAS group, expression levels of Ndufa12, IL-6, TNF-α, and Vdac1 were significantly reduced, while a marked elevation was found in MIP2. In addition, results found in an animal model showed that SAAS could obviously facilitate motor function recovery of mice after spinal cord injury, and it had a good protective effect on spinal cord tissue and neuron cells. CONCLUSION: As a result, the present study clarified both the protective effect of SAAS on neurons after spinal cord injury and the anti-inflammatory effect of microglia, which is expected to serve as a theoretical basis for clinical treatment.
Authors: Stephani A Davis; Sheed Itaman; Christopher M Khalid-Janney; Justin A Sherard; James A Dowell; Nigel J Cairns; Michael A Gitcho Journal: Neurosci Lett Date: 2018-04-30 Impact factor: 3.046