Activation of Autophagy Pathway Suppresses the Expression of iNOS, IL6 and Cell Death of LPS-Stimulated Microglia Cells.

Microglia play a role in maintaining and resolving brain tissue homeostasis. In pathological conditions, microglia release pro-inflammatory cytokines and cytotoxic factors, which aggravate the progression of neurodegenerative diseases. Autophagy pathway might be involved in the production of pro-inflammatory cytokines and cytotoxic factors in microglia, though details of the mechanism remain largely unknown. In the present study, we examined the role of the autophagy pathway in activated BV2 microglia cells. In BV2 cells, rapamycin treatment activated the formation of anti-LC3-labeled autophagosomes, whereas the ATG5 depletion using siRNA-ATG5 prevented the formation of LC3-labeled autophagosomes, indicating that BV2 cells exhibit an active classical autophagy system. When treated with LPS, BV2 cells expressed an increase of anti-LC3-labeled dots. The levels of LC3-labeled dots were not suppressed, instead tended to be enhanced, by the inhibition of the autophagy pathway with siRNA-ATG5 or wortmannin, suggesting that LPS-induced LC3-labeled dots in nature were distinct from the typical autophagosomes. The levels of LPS-induced expression of iNOS and IL6 were suppressed by treatment with rapamycin, and conversely, their expressions were enhanced by siRNA-ATG5 treatment. Moreover, the activation of the autophagy pathway using rapamycin inhibited cell death of LPS-stimulated microglia. These results suggest that although microglia possess a typical autophagy pathway, the glial cells express a non-typical autophagy pathway in response to LPS, and the activation of the autophagy pathway suppresses the expression of iNOS and IL6, and the cell death of LPS-stimulated microglia.

INTRODUCTION

Microglia are macrophage-like resident immune cells in the brain. Microgia are activated in response to various cellular factors, including cytokines, chemokines, nitric oxide (NO), and reactive oxygen intermediates and by pathological insults including truma, stroke, and infection (Gehrmann ; Magazine ; Perry . In normal brain conditions, microglia play a role in the maintenance and resolution of brain tissue homeostasis. In pathological conditions, microglia release high levels of pro-inflammatory mediators and cytotoxic factors, which may activate nearby microglia and thus propagate the production of pro-inflammatory factors in a vicious cycle (Hanisch, 2002; Smith . Prolonged, or excessive activation of microglia may produce inflammatory reactions in the brain, which are believed to aggravate the progression of neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and ALS (McGeer ; Matsumoto ; Raine, 1994; Henkel . Pharmacological or genetic inhibition of the action of proinflammatory cytokines and NO causes neuroprotective effects in cell cultures in vitro, and in animal models of neurodegenerative diseases (Iadecola ; Mayo and Stein, 2007; Beurel and Jope, 2009).

In various types of cells, defective cytoplasmic materials or infective pathogens are engulfed into double-membrane vesicles known as autophagosomes, which are then fused with lysosomes and degraded or recycled. This process, called autophagy, plays a major role in resolving dysfunctional cellular proteins and organelles resulting from many physiological and pathological conditions (Levine and Klionsky, 2004). Proper activation of the autophagy pathway ensures cell survival and function, while excess or reduced activation of the autophagy pathway may cause pathological consequences including the failure of removing pathogenic materials and increased cell death in neurodegenerative diseases (Nixon and Yang, 2011; Rosello . The autophagy pathway is regulated by the signaling pathway regulated by the mammalian target of rapamycin (mTOR), which is a serine/threonine protein kinase. mTOR normally functions as a negative regulator of the autophagy pathway. Inhibition of mTOR leads to turn on the autophagy pathway. Rapamycin, which is a lipophilic macrolide isolated from a strain of Streptomyces hygroscopicus, is a potent suppressor of mTOR (Zoncu . Typically, cells treated with rapamycin express the autophagy pathway. Upon induction of autophagy, microtuble-associated protein light chain 3 (LC3), a mammalian homolog of yeast Atg8, is conjugated to phosphatidyl ethanolamine (PE) by aid of autophagy-regulating gene products, such as ATG5 and ATG7. The lipidation of Gly120 of LC3 with PE converts the LC3-I form to LC3-II form, which is then then targeted to autophagic membranes (Nakagawa ; Virgin and Levine, 2009). Recently it was reported that LPS treatment in macrophages produces aggresome-like induced structures (ALIS), which is independent from the classic autophagic mechanism, and the ALIS formation was regulated by the enhanced expression of Nrf2 and p62 (Fujita . Similar to ALIS in LPS-stimulated macrophages, dendritic cells treated with LPS, produced dendritic cell aggresome-like induced structures (DALIS). The DALIS formation was prevented by the inhibition of new protein synthesis, whereas abnormal protein synthesis enhanced DALIS (Lelouard ; Lelouard . Although changes in LC3 localization have been used to measure autophagy, LC3 formation can be induced independent from the classic autophagic mechanism.

Autophagy influences the physiological and pathological conditions of many immune cells including macrophages. Autophagy plays a critical role in the pathogen elimination and cytokines production of macrophages (Singh ; Saitoh ; Nakahira ; Lee . Increased activation of IL-1β and IL-18 has also been observed in macrophages and monocytes isolated from mice genetically deficient in atg16l1, Beclin 1, or LC3B (Saitoh ; Nakahira . Therefore, it might be assumed that the autophagy pathway plays a role in microglia, the resident immune cells carrying many macrophage-like properties in the brain. However, autophagy formation and regulation in microglia, and its effect on the production of pro-inflammatory and cytotoxic factors are largely unknown. In the present study, we demonstrated that BV2 microglia cells express both classical and modified autophagy processes, and the activation of autophagy process mitigates the expression of IL6, iNOS and the cell death of BV2 cells challenged with LPS.

MATERIALS AND METHODS

Cell cultures and transfection

BV2 cells were cultured as described previously (Yu . In brief, BV2 cells were maintained in DMEM (GIBCO-BRL) supplemented with 10% fetal bovine serum (GIBCO-BRL), 1% L-glutamine, penicillin (50 U/L), and streptomycin (50 mg/ml). For transfection with small interfering RNA (siRNA), cells were plated in 6-well plates at 2×105 cells/well. Plated cells were grown in DMEM with 10% FBS overnight, and were then transfected with siRNA-mATG5 (#L-064838: Dhamacon, Lafayette, CO, USA; #1325012, 1325013 and 1325014: Bioneer, Daejon, Korea) using the transfection reagent Lipofectamine RNAiMAX (Invitrogen, California, USA) according to the manufacturer’s protocol, 24 h prior to treatment with drugs.

Production of BV2 stable cell lines expressing GFP-LC3

Production of stable cell lines, and clonal selection were performed as described previously (Sims . The pEGFP-LC3 plasmid was described (Kabeya . Total pEGFP-LC3 plasmid 500 ng was mixed with 1 μl of lipofectamine (Invitrogen) and incubated for 5 h, by following the manufacture’s suggestion. The plasmid mix was then used to transfect BV2 cells growing in DMEM (GIBCO-BRL, Rockville, MA, USA) supplemented with 10% fetal bovine serum (GIBCO-BRL), 1% L-glutamine, penicillin (50 U/L), and streptomycin (50 mg/ml) in 24-well plates. After 48 h, the culture media was changed in every 3 days, replacing with fresh DMEM, containing G418 of increasing concentrations (400 μg/ml, 600 μg/ml and 800 μg/ml) throughout the 3-weeks period. Finally, 3 GFP-positive BV2 colonies were obtained. Each colony was selected and expanded in DMEM containing G418 at 400 μg/ml and cloned cells were named BV2-GFP-LC3 stable cells (or BV2-LC3 cells). The BV2-GFP-LC3 stable cells that express GFP at the highest level were used in the present study.

Autophagy assay

Autophagy Assay was performed by following the procedures described previously (Xu ; Lee with a minor modification. BV2 cells or BV2-LC3 cells growing in a 6-well plate for 24 h were treated with rapamycin (#R0395; Sigma, St. Louis, MO, USA), LPS (#L4516; Sigma, St. Louis, MO, USA), or wortmannin (#W1628; Sigma, St. Louis, MO, USA) for the indicated times. For quantification of LC3-positive dots, BV2 cells cultured on coverslips (BD) were fixed with 4% paraformaldehyde in PBS. After permeabilization with 0.5% Triton X-100 for 10 min, fixed cells were blocked with 5% BSA for 1 h, and then stained with anti-LC3 antibody (1:100 dilution; Abgent, San Diego, CA, USA) overnight. After washing, they were then incubated with secondary anti-mouse IgG-FITC for 1 h. The coverslips were mounted on slides using DAPI-containing mounting solution (Vector Laboratories, Burlingame, CA). LC3 dot images were analyzed using a confocal microscope (Zeiss LSM 510) and using a fluorescent microscope (Olympus, Tokyo, Japan) equipped with the TOMORO image analysis program (TOMORO Scope Eye™; Techsan Community, Seoul, Korea). The formation of GFP-LC3 dots in BV2-LC3 cells was analyzed using a fluorescent microscope. The ratio of the area of LC3-positive dots vs. the area of total cells was calculated.

Western blot analysis

Cellular proteins were extracted with RIPA buffer [50 mM Tris (pH 8.0), 150 mM NaCl, 1 mM EDTA, 10% glycerol, 2% Triton X-100 (Im , and a protein inhibitor mixture (Roche, Basel, Swiss)]. Total 30 μg of proteins per each lane was subjected to immune blot analysis. Mouse monoclonal to LC3 (1:100 dilution; Abgent) and α-tubulin were used.

Real-time quantitative PCR analysis

Total RNA was extracted using the TRIzol reagent. The following primers were used: 5’-GCTGCCCTATACCCACATCT-3’ and 5’-CGCCTTCATCCGAGAAAC-3’ for p62/SQSTM1; 5’-GATGGATGCTACCAA ACTGGAT-3’ and 5’-GATGGATGCTACCAA ACTGGAT-3’ for IL-6; 5’-CTTGCCACGGACGAGAC-3’ and 5’-TCATTGTACTCTGAGGGCTGA-3’ for iNOS; 5’-AAGTCTGTCCTTCCGCAGTC-3’ and 5’-TGAAGAAAGTTATCTGGGT AGCTCA-3’ for ATG5; 5′- AGACATGGAGTCATAGGCTCTG-3’ and 5’-CCATTTTCCTTCTTGTGGAGCA-3’ for IL-12; 5’-TCACACGAGATGAGCTTAGGGCAA-3’ and 5’-TACAGTTCTGG GCGGCGACTTTAT-3’ for Nrf2; 5’-CGTCAGCCGATTTGCTATCT-3’ and 5’-CGGACTCCGCAAAGTCTAAG-3’ for TNF-α; 5’-GCCCATCCTCTGTGACTCAT-3’ and 5’-AGGCCACAGGTATTTTGTCG-3’ for IL-1β; 5’ -TTAAAAACCTGTATCGGAACCAA-3’ and 5’-GCATTAGCTTCAGATTTACGGGT-3’ for MCP1; 5’- ATGAACGCTACACACTGCATC-3’ and 5’-CCATC CTTTTGCCAGTTCCTC-3’ for IFN-γ; 5’-CTGGAGCAGCTGAATGGAAAG-3’ and 5’-TCTCCGTCATCTCATAGGGA-3’ for IFN-β; 5’-TGTCCGTCGTGGATCTGAC-3’ and 5’-CCTGCTTCACCACCTTCTTG-3’ for GAPDH. Fold-changes were calculated by using the ΔΔCT method and the experiments repeated at least three times.

Nitrite quantification

The production of NO was determined through the quantification of nitrite from the spontaneous oxidation of NO in media after 24 h. Accumulated nitrite was quantified using a colorimetric reaction with Griess reagent, and the absorbance for which was measured at 570 nm by a microplate reader (Spectra Max 190, Molecular Devices).

Cell death assay

Cell death was measured by the activity of lactate dehydrogenase (LDH) released to the culture medium, using a LDH assay kit (Roche Diagnostics Corporation) by following the manufacturer’s instructions. Culture media were collected 24 h after LPS and rapamycin treatment. The absorbance of the samples was measured at 492 nm by a microplate reader (Spectra Max 190, Molecular Devices).

Mice and stereotaxic injection of LPS

Male C57BL/6J mice at 8-12-weeks-old which had been raised in our laboratory were used for this experiment. Mice were housed in a temperature- (23℃) and humidity-(45-55%) controlled environment with a 12/12 h dark-light cycle (lights on 7:00 A.M and off on 7:00 P.M.). Stereotaxic injection was performed as described previously (Han . Mice were anesthetized with ketamine (100 mg/kg body weight) and xylazine hydrochloride (13 mg/kg body weight). The head of each mouse was secured in a Benchmark stereotaxic instrument (Coretech Holdings Company, St. Louis, MO, USA). LPS (1 μg in 3 μl) was unilaterally injected at the prefrontal cortex near the dorsal striatum (AP: +1.0, ML: +-1.5 mm, DV: -3.6 mm) at the speed of 0.5 μl/min. After surgery, mice were placed on a warm plate (37℃) and then individually housed in normal plastic cages, and after 24 h, they were sacrificed.

Immunohistochemistry

Brain tissue was fixed in 4% paraformaldehyde solution and sectioned to a thickness of 40 μm by using vitratom. Free-floating serial sections were rinsed three times for 10 min in PBS and then pretreated for 30 min in PBS containing 3% H2O2. The sections were then rinsed in PBS containing 0.1% triton X-100, and incubated for 1 h at room temperature in PBS containing 2% BSA and 2% horse serum. Next, the sections were incubated overnight at 4℃ with PBS containing 2% BSA, 2% horse serum and the following primary antibodies: IL6 (1:200; Biolegend, San Diego, California, USA), iNOS (1:1,000; Transduction Laboratory, San Diego, California, USA), and Iba1 (1:1000; Wako, Osaka, Japan). The sections were then washed by PBST and incubated by biotin-conjugated secondary antibodies (1:200; Vector Laboratories). Sections were incubated for 1 h in avidin-biotin complex solution (Vector Laboratories). After washing, the signal was detected by incubating sections in 0.5 mg/ml 3,3’ diaminobenzidine in PBS containing 0.3% H2O2. Sections mounted on gelatin-coated slides, and viewed under a bright-field microscope (Olympus).

Statistical analysis

Two-sample comparisons were carried out using the Student’s t-test, while multiple comparisons were made using one-way ANOVA followed by post-hoc tests to compare selected pairs of data. SPSS 16.0 (SPSS Inc., Chicago, IL, USA) were used to perform statistical analyses. All data are presented as the mean ± S.E.M., and statistical differences were accepted at the 5% level.

RESULTS

BV2 microglia cells have the functional classical autophagy pathway

To understand the mechanism of autophagy in microglia, BV2 microglia cells were treated with rapamycin, which is a classic autophagy inducer. Incubation of BV2 cells with rapamycin (100 nM) for 6 h led to the redistribution of microtubule-associated protein light-chain 3 (LC3/Atg8) from the diffused pattern to punctuated patterns, forming typical autophagosome-like dots. The autophagosome-like dots were visualized by staining with anti-LC3 antibody on a confocal microscopic field (Fig. 1A). Western blot analysis also confirmed that rapamycin treatment increased the level of LC3 type II, an indicator of autophagosome formation (Nakagawa (Fig. 1B). A computer-aid image analysis led to the confirmation that the formation of anti-LC3-labeled autophagosome-like dots was increased after treatment with rapamycin (Fig. 1C). Consistent with these results, rapamycin treatment of the BV2-LC3 stable cells, which were established in the present study by transfection with EGFP-LC3 plasmid and a subsequent clonal selection, showed enhanced formation of EGFP-LC3-labeled dots (Fig. 1D). When BV2 cells were transfected with siRNA-ATG5 to block the autophagy pathway, the formation of rapamycin-induced EGFP-LC3-labeled dots was completely suppressed (Fig. 2). Together, these results indicate that BV2 microglia cells have an active classical autophagy system.

Fig. 1.

Rapamycin treatment induced activation of the autophagy process in BV2 microglia cells. (A) Confocal microscopic images showing the formation of anti-LC-labeled dots in rapamycin-stimulated BV2 cells. Cells were treated with rapamycin (100 nM) for 6 h and were stained with anti-LC3 antibody. (B) Western blots showing rapamycin-dependent increase of the ratio of LC3-II/LC3-I and their quantified data. Western blot analysis was performed 6 h after treatment with rapamycin using anti-LC3 antibody. The concentration of rapamycin was 10 or 100 nM. The band intensity of LC3-II and LC3-I was measured by a densitometer. The graph represented the ratio of LC3-II/LC3-I. (C) Representative fluoresence microscopic images showing anti-LC3-labeled dots in BV2 cells before and after treatment with rapamycin (100 nM) and their quantified data. The percentage of total LC3-dot area per total cell area was quantified using by an image analysis program described in Materials and Methods. (D) Representative fluorescence microscopic images showing the distribution of GFP-LC3 in BV2-LC3 stable cells after treatment with rapamycin. The percent area of GFP-LC3 fluorescence dots per total cell area was quantified 6 h after treatment with rapamycin (100 nM). Data are presented as the means ± SEM. ** denotes difference between control cells and rapamycin treated cells at p<0.01. Scale bar; 10 μm.

Fig. 2.

Rapamycin-induced autophagy-like dots in BV2 cells were suppressed by siRNA-ATG5. (A) Real-time PCR data showing the inhibition of the ATG5 gene expression in BV2 cells transfected with siRNA-ATG5. The expression levels of ATG5 were examined 24 h after transfection with siRNA-ATG5. The PCR products were also visualized on agarose gel. (B, C) Representative fluorescence microscopic images showing GFP-LC3-labeled dots in control BV2-LC3-stable cells (Control), BV2-LC3-stable cells treated with rapamycin (100 nM), and BV2-LC3-stable cells treated with rapamycin and siRNA-ATG5 (Rapa+siATG5). siRNA-ATG5 was transfected 24 h prior to rapamycin treatment. Quantified areas of GFP-LC3-labeled dots are presented (C). The percent area of GFP-LC3 fluorescence dots was quantified 6 h after rapamycin treatment (100 nM). Data are presented as the means ± SEM. ** denotes difference between indicated groups at p<0.01.

LPS-activated BV2 microglia cells produced LC3-labeled dots, which were distinct from the classical autophagic machinery

Next, we investigated the role of the autophagy pathway in BV2 cells which were activated with lipopolysaccharide (LPS), a strong activator of microglia cells. LPS treatment increased the levels of anti-LC3-labeled dots in BV2 cells (Fig. 3A, B) and also EGFP-LC3-labeled dots in BV2-LC3 stable cells (Fig. 3C). The formation of EGFP-LC3-labeled dots in LPS-stimulated BV2-LC3 stable cells increased in a time-dependent manner, peaking after 4 h (Fig. 3D). However, LPS-induced EGFP-LC3-labeled dots in BV2-LC3 stable cells were not suppressed by siRNA-ATG5 (Fig. 3E, 3F), nor by ‘wortmannin’- a compound that prevents autophagosomes formation by inhibiting the PI3K pathway. Further analysis revealed that co-treatment of BV2 cells with LPS and rapamycin doubled the level of anti-LC3-labeled dots induced by LPS alone (Fig. 3G, 3H). These results suggest that LPS-activated microglia express the formation of LC3-labeled dots, but the LC3-labeled dots are distinct from the typical autophagosomes.

Fig. 3.

LPS-induced activation of microglia expressed aggresome-like LC3-labeled dots. (A) Confocal microscopic immunofluosence images (top panels) showing anti-LC3-labeled dots in BV2 cells after stimulation with LPS. Anti-LC3 immunofluosence images were overlayed onto phase-contrast microscopic images (bottom panels). LPS (100 ng/ml) was treated for 6 h. (B) Representative fluoresence microscopic images showing anti-LC3-labeled dots in BV2 cells after treatment with LPS (100 ng/ml) and their quantified data. The total LC3-labled dot area per total cell area was quantified using by an image analysis program. (C) Representative fluorescence microscopic images showing the distribution of GFP-LC3-labeled dots in BV2-LC3 stable cells after treatment with LPS (100 ng/ml). The percent area of GFP-LC3 fluorescence dots was quantified 6 h after treatment with LPS. (D) Time-dependent increase and saturation in the formation of GFP-LC3-labeled dots in LPS-stimulated BV2-LC3 stable cells. Cells were incubated in LPS (100 ng/ml) for the indicated time. The percentage of total LC3-dot area was quantified with respect to total cell areas. (E, F) LPS-induced GFP-LC3-labeled dots in BV2-LC3 stable cells were not suppressed by siRNA-ATG5 (E) or wortmanin (F). siRNA-ATG5 was transfected 24 h prior to treatment of LPS or wortmanin. LPS (100 ng/ml) and wortmannin (200 nM) were treated for 6 h. The percentage of the LC3-dot area per total cell area is presented. (G, H) LPS and rapamycin independently increased GFP-LC3-labeled dots in BV2 cells treated with LPS, LPS+rapamycin, orLPS+siRNA-ATG5. LPS (100 ng/ml) or rapamycin (100 nM) was treated for 6 h. siRNA-ATG5 was transfected 24 h prior to treatment with LPS. (I-J) Real-time PCR data showing LPS-induced increase in the levels of Nrf2 (I) and p62 (J). LPS (100 ng/ml) was treated for 6 h and total RNA was harvested immediately after that. Data are presented as the means ± SEM. * and ** denote difference between indicated groups at p<0.05 and p<0.01, respectively. ns, no significant difference.

The formation of aggresome-like induced structures (ALIS) in LPS-treated macrophages, which is distinguished from the classic autophagic machinery, was regulated by the enhanced expression of Nrf2 and p62 (Fujita . Real-time PCR analysis showed that LPS-activated BV2 cells had the increased the expression of Nrf2 and p62 (Fig. 3I, J). Although this data alone was indirect, it also supports the possibility that LPS-activated microglia cells express LC3-labeled dots which are distinct from the classical autophagic machinery.

The autophagy pathway mitigated the pathological cellular responses of LPS-stimulated microglia

Next, we examined the expression profiles of various cytokines in LPS-activated BV2 cells. Activation of the autophagy pathway by rapamycin suppressed the levels of LPS-induced expression of iNOS, IL-6, MCP1, and IL1β, but increased the levels of LPS-induced expression of IL12, INFγ, INFβ, and TNFα (Fig. 4, Supplemental Fig. 1).

Fig. 4.

LPS-induced expression of IL6 and iNOS in BV2 cells was modulated by the autophage pathway. (A, B) Real-time PCR data showing the transcription levels of iNOS (A) and IL6 (B) in BV2 cells stimulated with LPS or rapamycin. LPS (100 ng/ml) or rapamycin (100 nM) was treated for 6 h. siRNA-ATG5 was transfected 24 h prior to treatment with LPS. Data are presented as the means ± SEM. * and ** denote difference between control-LPS and indicated group at p<0.05 and p<0.01, respectively. (C) NO levels produced in BV2 cells after stimulation with LPS (100 or 1,000 ng/ml), LPS+rapamycin (100 nM), LPS+siRNA-ATG5. siRNA-ATG5 was transfected 24 h prior to treatment with LPS as above. NO levels were measured by Griess method 24 h after LPS treatment. Data are presented as the means ± SEM. # and ## denote difference between control-LPS (100 ng/ml) and indicated group at p<0.05 and p<0.01, respectively. * and ** denote difference between control-LPS (1,000 ng/ml) and indicated group at p<0.05 and p<0.01, respectively.

The levels of LPS-induced expression of iNOS and IL6 were increased when the autophagy pathway was blocked by siRNA-ATG5 (Fig. 4A, B). Consistent with the marked induction of iNOS expression after LPS stimulation, NO levels produced from LPS-stimulated BV2 cells were enhanced when ATG5 was repressed using siRNA-ATG5, and conversely, NO levels were partially suppressed when the autophagy pathway was activated using rapamycin (Fig. 4C).

The results of the autophagy-dependent suppression of LPS-induced expression of iNOS and IL6 in BV2 cells (Fig. 4A, B), and the expression of these genes in activated microglia in the brain (Supplemental Fig. 2) led us to examine the effect of autophagy on cell death of LPS-stimulated microglia. Treatment of BV2 cells with LPS at concentrations of 100 and 1,000 μg/ml for 24 h produced 54.4% and 79% cell death, respectively, whereas the addition of rapamycin suppressed the levels of LPS-induced cell death in a dose-dependent manner (Fig. 5). These results suggest that the activation of the autophagy pathway in microglia cells confers a protective effect against the cell death of LPS-stimulated microglia.

Fig. 5.

LPS-induced cytotoxicity was diminished by the activation of the autophagy pathway. The cell death levels of BV2 cells in response to LPS in the presence of varying doses of rapamycin. Rapamycin suppressed the LPS-induced cytotoxicity in a dose-dependent manner. LPS (100 or 1,000 μg/ml) and rapamycin (10, 100, or 1,000 nM) were treated for 24 h. Cell death was measured by LDH assay. Data are presented as the means ± SEM. ** denotes difference between control and indicated group at each LPS dose (LPS, 100 or 1,000 μg/ml alone) at p<0.01.

DISCUSSION

In the present study, we demonstrated that treatment of microglia cells with rapamycin led to form LC3-labeled dots, and that the formation of LC3-labeled dots was blocked by siRNA-ATG5, indicating that microglia actively express the typical autophagy pathway described in other cell types (Akar ; Yuan . When activated by LPS, microglia also expressed LC3-labeled dots. However, the formation of LPS-induced LC3-labeled dots was not blocked by siRNA-ATG5, suggesting that LPS-induced LC3-labeled dots have differentiating features from the classic autophagic mechanism.

We speculate that LC3-labeled dots formed in LPS-stimulated BV2 cells have a similarity to ALIS (aggresome-like induced structures) described in macrophages (Szeto ; Fujita and DALIS (dendritic cell aggresome-like induced structures) in dendritic cells (Lelouard ; Lelouard ; Canadien . The activation of TLR receptors (Lu by LPS promoted autophagy process in macrophages (Xu ; Shi and Kehrl, 2008). LPS-stimulated macrophages expressed ALIS, which appeared with LC3-labeled dot-like structure. However, ALIS was not advanced to the stage of lysosomal degradation, thus making a distinction between ALIS and classical autophagosomes. ALIS formation in LPS-stimulated macrophages was regulated by the increased expression of Nfr2 and p62 (Fujita . Similar to ALIS in LPS-stimulated macrophages, LPS-stimulated BV2 cells expressed the formation of a non-classical type LC3-labeled dots (Fig. 3A-G), and showed increased expression of Nfr2 and p62 (Fig. 3I, 3J). The DALIS in dendritic cells functions as a compartment for the sequestration of misfolded proteins. In LPS-stimulated dendritic cells, DALIS appeared at 4 h after LPS stimulation, reaching its peak at 8 h, and diminished (Lelouard . The DALIS formation was prevented by the inhibition of new protein synthesis, whereas abnormal protein synthesis was found to enhance DALIS (Lelouard ; Lelouard . ALIS formation in LPS-stimulated macrophages steadily increased up to 24 h, whereas LPS-induced LC3-dot formation in BV2 microglia cells peaked at 4 h and thereafter slowly decreased. These results suggest that the time course of LPS-induced LC3-dot formation in microglia was more similar to DALIS in dendritic cells than to ALIS in macrophages, although the specific mechanism of its action remains unknown.

Autophagy has been suggested to be a neuroprotective mechanism due to its role in the clearance of harmful protein aggregates and organelles in the brain (Ravikumar ; Sarkar and Rubinsztein, 2008). In particular, rapamycin was helpful for HIV dementia through the stimulation of autophagy (Alirezaei . In the present study, we demonstrated that autophagy blockage by siRNA-ATG5 increased the expression of IL6 and iNOS in LPS-treated microglia, whereas autophagy induction with rapamycin decreased the expression of these genes (Fig. 3). Furthermore, autophagy induction with rapamycin decreased cell death of LPS-treated microglia (Fig. 4). Thus, our results support the notion that the activation of the autophagy pathway aids LPS-stimulated microglia cells in protecting against the cell death, probably through reducing the production of iNOS and IL6. Inducible NOS is an enzyme that produces toxic levels of nitric oxide (NO) (Gross and Wolin, 1995). NO produced by iNOS aggravates LPS and interferon-γ triggered cell death in N9 microglial cells and primary microglia cells (Mayo and Stein, 2007). Inhibition of iNOS using aminoguanidine had beneficial effects in the brain with focal cerebral ischemic damage in rats (Zhang ; Kim and Lee, 2007) and in the MPTP model of Parkinson disease (Wu . The susceptibility to cerebral ischemia also was decreased in mice lacking iNOS (Iadecola . The increased IL-6 contributes to behavioral deficits and neuronal loss in i.p injection of LPS model (Semmler and in Alzheimer’s model (Strauss ; Akiyama ; Eriksson . These results suggest that regulation of iNOS and IL6 production by the regulation of the autophagy pathway will be a valuable strategy to protect the brain in pathological states.