| Literature DB >> 33074545 |
Miosotis Alicea-Delgado1, Samir A Bello-Melo2, José E García-Arrarás2.
Abstract
Functional studies on echinoderms have been reduced to the use of pharmacological treatments. The ability to modulate the genetic expression of regenerating tissues can elucidate potential effectors during this process. Here we describe an effective transfection protocol that allows the introduction of Dicer-substrate interference RNAs (DsiRNAs) for the modulation of gene expression and its characterization during regeneration.Entities:
Keywords: DsiRNA; Echinoderm; Electroporation; Gut rudiment; RNAi; Regeneration; Sea Cucumber
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Year: 2021 PMID： 33074545 PMCID： PMC9503136 DOI： 10.1007/978-1-0716-0974-3_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745