Najat El-Kurdi1, Hesham Abdulla2, Amro Hanora3. 1. Department of Aquaculture Biotechnology, Fish Farming and Technology Institute, Suez Canal University, Ismailia, Egypt. nagat.elkurdi.fish@suez.edu.eg. 2. Department of Botany, Faculty of Science, Suez Canal University, Ismailia, Egypt. 3. Department of Microbiology & Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
Abstract
OBJECTIVES: To screen for a variety of marine bacteria with anti-quorum sensing and anti-biofilm activities. RESULTS: Among 188 bacterial isolates from water, sediment, and corals in the Red Sea region, approximately 35% (65 isolates) of the isolates displayed a significant degradation in the purple pigment of the bioreporter strain without affecting cell growth. The quorum quenching bacteria obtained from coral-associated bacteria were 66.2% out of the total isolates. The PCR amplification results revealed that the recorded Acyl Homoserine lactone (AHL) inhibition by 91% of the anti-QS marine bacteria was not due to lactonase activity. On the other hand, lactonase genes were recorded only in the remaining 9% (6 isolates) and those were belonging to genus Bacillus, Nocardiopsis, and Enterobacter based on 16S rRNA gene sequences. The results also showed that marine bacteria with anti-QS activity inhibited 67% of the biofilm formed by Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio alginolyticus. The computational profiling analysis confirmed the presence of the functional region in the detected genes. CONCLUSION: Coral microbial communities are rich sources for pharmacologically important natural products with anti-quorum sensing and anti-biofilm activities.
OBJECTIVES: To screen for a variety of marine bacteria with anti-quorum sensing and anti-biofilm activities. RESULTS: Among 188 bacterial isolates from water, sediment, and corals in the Red Sea region, approximately 35% (65 isolates) of the isolates displayed a significant degradation in the purple pigment of the bioreporter strain without affecting cell growth. The quorum quenching bacteria obtained from coral-associated bacteria were 66.2% out of the total isolates. The PCR amplification results revealed that the recorded Acyl Homoserine lactone (AHL) inhibition by 91% of the anti-QS marine bacteria was not due to lactonase activity. On the other hand, lactonase genes were recorded only in the remaining 9% (6 isolates) and those were belonging to genus Bacillus, Nocardiopsis, and Enterobacter based on 16S rRNA gene sequences. The results also showed that marine bacteria with anti-QS activity inhibited 67% of the biofilm formed by Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio alginolyticus. The computational profiling analysis confirmed the presence of the functional region in the detected genes. CONCLUSION: Coral microbial communities are rich sources for pharmacologically important natural products with anti-quorum sensing and anti-biofilm activities.
Entities:
Keywords:
Anti-biofilm; Coral-associated bacteria; Lactonase; Quorum quenching bacteria; Red sea
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