Literature DB >> 33030237

Leginon: New features and applications.

Anchi Cheng1, Carl Negro1, Jessica F Bruhn2, William J Rice3,4, Sargis Dallakyan1, Edward T Eng1, David G Waterman5,6, Clinton S Potter1,7, Bridget Carragher1,7.   

Abstract

Leginon is a system for automated data acquisition from a transmission electron microscope. Here we provide an updated summary of the overall Leginon architecture and an update of the current state of the package. We also highlight a few recent developments to provide some concrete examples and use cases.
© 2020 The Protein Society.

Entities:  

Keywords:  automation; cryoEM; microED

Mesh:

Year:  2020        PMID: 33030237      PMCID: PMC7737759          DOI: 10.1002/pro.3967

Source DB:  PubMed          Journal:  Protein Sci        ISSN: 0961-8368            Impact factor:   6.725


  25 in total

1.  Automated cryoEM data acquisition and analysis of 284742 particles of GroEL.

Authors:  Scott M Stagg; Gabriel C Lander; James Pulokas; Denis Fellmann; Anchi Cheng; Joel D Quispe; Satya P Mallick; Radomir M Avila; Bridget Carragher; Clinton S Potter
Journal:  J Struct Biol       Date:  2006-05-22       Impact factor: 2.867

2.  Towards automated screening of two-dimensional crystals.

Authors:  Anchi Cheng; Albert Leung; Denis Fellmann; Joel Quispe; Christian Suloway; James Pulokas; Priyanka D Abeyrathne; Joseph S Lam; Bridget Carragher; Clinton S Potter
Journal:  J Struct Biol       Date:  2007-09-25       Impact factor: 2.867

3.  Appion: an integrated, database-driven pipeline to facilitate EM image processing.

Authors:  Gabriel C Lander; Scott M Stagg; Neil R Voss; Anchi Cheng; Denis Fellmann; James Pulokas; Craig Yoshioka; Christopher Irving; Anke Mulder; Pick-Wei Lau; Dmitry Lyumkis; Clinton S Potter; Bridget Carragher
Journal:  J Struct Biol       Date:  2009-04       Impact factor: 2.867

4.  Addressing preferred specimen orientation in single-particle cryo-EM through tilting.

Authors:  Yong Zi Tan; Philip R Baldwin; Joseph H Davis; James R Williamson; Clinton S Potter; Bridget Carragher; Dmitry Lyumkis
Journal:  Nat Methods       Date:  2017-07-03       Impact factor: 28.547

5.  Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.

Authors:  M Jason de la Cruz; Johan Hattne; Dan Shi; Paul Seidler; Jose Rodriguez; Francis E Reyes; Michael R Sawaya; Duilio Cascio; Simon C Weiss; Sun Kyung Kim; Cynthia S Hinck; Andrew P Hinck; Guillermo Calero; David Eisenberg; Tamir Gonen
Journal:  Nat Methods       Date:  2017-02-13       Impact factor: 28.547

6.  DIALS: implementation and evaluation of a new integration package.

Authors:  Graeme Winter; David G Waterman; James M Parkhurst; Aaron S Brewster; Richard J Gildea; Markus Gerstel; Luis Fuentes-Montero; Melanie Vollmar; Tara Michels-Clark; Iris D Young; Nicholas K Sauter; Gwyndaf Evans
Journal:  Acta Crystallogr D Struct Biol       Date:  2018-02-01       Impact factor: 7.652

7.  The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination.

Authors:  Christopher G Jones; Michael W Martynowycz; Johan Hattne; Tyler J Fulton; Brian M Stoltz; Jose A Rodriguez; Hosea M Nelson; Tamir Gonen
Journal:  ACS Cent Sci       Date:  2018-11-02       Impact factor: 14.553

8.  Benchmarking cryo-EM Single Particle Analysis Workflow.

Authors:  Laura Y Kim; William J Rice; Edward T Eng; Mykhailo Kopylov; Anchi Cheng; Ashleigh M Raczkowski; Kelsey D Jordan; Daija Bobe; Clinton S Potter; Bridget Carragher
Journal:  Front Mol Biosci       Date:  2018-06-04

9.  High-resolution structure determination by continuous-rotation data collection in MicroED.

Authors:  Brent L Nannenga; Dan Shi; Andrew G W Leslie; Tamir Gonen
Journal:  Nat Methods       Date:  2014-08-03       Impact factor: 28.547

10.  High-resolution cryo-EM reconstructions in the presence of substantial aberrations.

Authors:  Raquel Bromberg; Yirui Guo; Dominika Borek; Zbyszek Otwinowski
Journal:  IUCrJ       Date:  2020-03-26       Impact factor: 5.588

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  13 in total

1.  Structural and functional analysis of the SET3 histone deacetylase complex.

Authors:  Alexis A Reyes; Susan Fishbain; Yuan He
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2022-02-23       Impact factor: 1.056

Review 2.  Broadening access to cryoEM through centralized facilities.

Authors:  Christina M Zimanyi; Mykhailo Kopylov; Clinton S Potter; Bridget Carragher; Edward T Eng
Journal:  Trends Biochem Sci       Date:  2022-02       Impact factor: 13.807

3.  Asymmetric Structures and Conformational Plasticity of the Uncleaved Full-Length Human Immunodeficiency Virus Envelope Glycoprotein Trimer.

Authors:  Shijian Zhang; Kunyu Wang; Wei Li Wang; Hanh T Nguyen; Shuobing Chen; Maolin Lu; Eden P Go; Haitao Ding; Robert T Steinbock; Heather Desaire; John C Kappes; Joseph Sodroski; Youdong Mao
Journal:  J Virol       Date:  2021-09-22       Impact factor: 5.103

4.  Structures of the T cell potassium channel Kv1.3 with immunoglobulin modulators.

Authors:  Purushotham Selvakumar; Ana I Fernández-Mariño; Nandish Khanra; Changhao He; Alice J Paquette; Bing Wang; Ruiqi Huang; Vaughn V Smider; William J Rice; Kenton J Swartz; Joel R Meyerson
Journal:  Nat Commun       Date:  2022-07-04       Impact factor: 17.694

5.  Optimized cryo-EM data-acquisition workflow by sample-thickness determination.

Authors:  Jan Rheinberger; Gert Oostergetel; Guenter P Resch; Cristina Paulino
Journal:  Acta Crystallogr D Struct Biol       Date:  2021-04-27       Impact factor: 7.652

6.  Coma-corrected rapid single-particle cryo-EM data collection on the CRYO ARM 300.

Authors:  Rouslan G Efremov; Annelore Stroobants
Journal:  Acta Crystallogr D Struct Biol       Date:  2021-04-14       Impact factor: 7.652

7.  Integrated wafer-scale manufacturing of electron cryomicroscopy specimen supports.

Authors:  Katerina Naydenova; Christopher J Russo
Journal:  Ultramicroscopy       Date:  2021-10-19       Impact factor: 2.689

8.  High-speed high-resolution data collection on a 200 keV cryo-TEM.

Authors:  Jared V Peck; Jonathan F Fay; Joshua D Strauss
Journal:  IUCrJ       Date:  2022-01-29       Impact factor: 4.769

9.  Multivalent interactions essential for lentiviral integrase function.

Authors:  Allison Ballandras-Colas; Vidya Chivukula; Dominika T Gruszka; Zelin Shan; Parmit K Singh; Valerie E Pye; Rebecca K McLean; Gregory J Bedwell; Wen Li; Andrea Nans; Nicola J Cook; Hind J Fadel; Eric M Poeschla; David J Griffiths; Javier Vargas; Ian A Taylor; Dmitry Lyumkis; Hasan Yardimci; Alan N Engelman; Peter Cherepanov
Journal:  Nat Commun       Date:  2022-05-03       Impact factor: 17.694

10.  Small Molecule Microcrystal Electron Diffraction for the Pharmaceutical Industry-Lessons Learned From Examining Over Fifty Samples.

Authors:  Jessica F Bruhn; Giovanna Scapin; Anchi Cheng; Brandon Q Mercado; David G Waterman; Thejusvi Ganesh; Sargis Dallakyan; Brandon N Read; Travis Nieusma; Kyle W Lucier; Megan L Mayer; Nicole J Chiang; Nicole Poweleit; Philip T McGilvray; Timothy S Wilson; Michael Mashore; Camille Hennessy; Sean Thomson; Bo Wang; Clinton S Potter; Bridget Carragher
Journal:  Front Mol Biosci       Date:  2021-07-12
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