Dianzhi Hou1,2,3, Qingyu Zhao1,2,3, Laraib Yousaf1,2,3, Yong Xue1,2, Qun Shen4,5,6. 1. College of Food Science and Nutritional Engineering, China Agricultural University, No.17, Qinghua East Road, Haidian District, Beijing, 100083, China. 2. National Engineering Research Center for Fruit and Vegetable Processing, Beijing, 100083, China. 3. Key Laboratory of Plant Protein and Grain Processing, China Agricultural University, Beijing, 100083, China. 4. College of Food Science and Nutritional Engineering, China Agricultural University, No.17, Qinghua East Road, Haidian District, Beijing, 100083, China. shenqun@cau.edu.cn. 5. National Engineering Research Center for Fruit and Vegetable Processing, Beijing, 100083, China. shenqun@cau.edu.cn. 6. Key Laboratory of Plant Protein and Grain Processing, China Agricultural University, Beijing, 100083, China. shenqun@cau.edu.cn.
Abstract
PURPOSE: Our recent study has reported that whole mung bean showed better beneficial effects on high-fat diet (HFD)-induced obesity and gut microbiota disorders when compared with the decorticated mung bean at the same intervention dose level, suggesting that the mung bean seed coat (MBC) may play a crucial role in its health benefits. This study aims to investigate whether MBC has beneficial benefits on the prevention of HFD-induced obesity and the modulation of gut microbiota in mice when it was supplemented in HFD. METHODS: Herein, male C57BL/6 J mice were fed with normal control diet, HFD, and HFD supplemented with MBC (3-6%, w/w) for 12 weeks. The changes in physiological, histological, biochemical parameters, serum endotoxin, proinflammatory cytokines, and gut microbiota composition of mice were determined to assess the ability of MBC to alleviate HFD-induced obesity and modulate gut microbiota disorders in mice. RESULTS: MBC supplementation exhibited significant reductions in the HFD-induced adiposity, fat accumulation, serum lipid levels, lipopolysaccharide, and proinflammatory cytokines concentrations (P < 0.05), which was accompanied by improvements in hepatic steatosis and adipocyte size. Especially, the elevated fasting blood glucose and insulin resistance were also significantly improved by MBC supplementation (P < 0.05). Furthermore, high-throughput sequencing of the 16S rRNA gene revealed that MBC could normalize HFD-induced gut microbiota dysbiosis. MBC not only could promote the bloom of Akkermansia, but also restore several HFD-dependent taxa (Blautia, Ruminiclostridium_9, Bilophila, and unclassified_f_Ruminococcaceae) back to normal status, co-occurring with the decreases in obesity-related indices. CONCLUSIONS: This study provides evidence that MBC may be mainly responsible for the beneficial effects of whole mung bean on preventing the HFD-induced changes, thus enlarging the application value of MBC.
PURPOSE: Our recent study has reported that whole mung bean showed better beneficial effects on high-fat diet (HFD)-induced obesity and gut microbiota disorders when compared with the decorticated mung bean at the same intervention dose level, suggesting that the mung bean seed coat (MBC) may play a crucial role in its health benefits. This study aims to investigate whether MBC has beneficial benefits on the prevention of HFD-induced obesity and the modulation of gut microbiota in mice when it was supplemented in HFD. METHODS: Herein, male C57BL/6 J mice were fed with normal control diet, HFD, and HFD supplemented with MBC (3-6%, w/w) for 12 weeks. The changes in physiological, histological, biochemical parameters, serum endotoxin, proinflammatory cytokines, and gut microbiota composition of mice were determined to assess the ability of MBC to alleviate HFD-induced obesity and modulate gut microbiota disorders in mice. RESULTS:MBC supplementation exhibited significant reductions in the HFD-induced adiposity, fat accumulation, serum lipid levels, lipopolysaccharide, and proinflammatory cytokines concentrations (P < 0.05), which was accompanied by improvements in hepatic steatosis and adipocyte size. Especially, the elevated fasting blood glucose and insulin resistance were also significantly improved by MBC supplementation (P < 0.05). Furthermore, high-throughput sequencing of the 16S rRNA gene revealed that MBC could normalize HFD-induced gut microbiota dysbiosis. MBC not only could promote the bloom of Akkermansia, but also restore several HFD-dependent taxa (Blautia, Ruminiclostridium_9, Bilophila, and unclassified_f_Ruminococcaceae) back to normal status, co-occurring with the decreases in obesity-related indices. CONCLUSIONS: This study provides evidence that MBC may be mainly responsible for the beneficial effects of whole mung bean on preventing the HFD-induced changes, thus enlarging the application value of MBC.
Entities:
Keywords:
Akkermansia; Gut microbiota; High-fat diet; Mung bean coat; Obesity
Authors: Vanessa K Ridaura; Jeremiah J Faith; Federico E Rey; Jiye Cheng; Alexis E Duncan; Andrew L Kau; Nicholas W Griffin; Vincent Lombard; Bernard Henrissat; James R Bain; Michael J Muehlbauer; Olga Ilkayeva; Clay F Semenkovich; Katsuhiko Funai; David K Hayashi; Barbara J Lyle; Margaret C Martini; Luke K Ursell; Jose C Clemente; William Van Treuren; William A Walters; Rob Knight; Christopher B Newgard; Andrew C Heath; Jeffrey I Gordon Journal: Science Date: 2013-09-06 Impact factor: 47.728