Pia Stockinger1, Andreas Berlin1, Daniel Kampik1, Christine Schmitt1, Jost Hillenkamp1, Jeffrey D Messinger2, Martina C Herwig-Carl3, Thomas Ach4,5. 1. Augenklink und Poliklinik, Universitätsklinikum Würzburg, Würzburg, Deutschland. 2. Department of Ophthalmology, University of Alabama at Birmingham, Birmingham, AL, USA. 3. Augenklinik, Universitätsklinikum Bonn, Ernst Abbe-Str. 2, 53127, Bonn, Deutschland. 4. Augenklink und Poliklinik, Universitätsklinikum Würzburg, Würzburg, Deutschland. thomas.ach@ukbonn.de. 5. Augenklinik, Universitätsklinikum Bonn, Ernst Abbe-Str. 2, 53127, Bonn, Deutschland. thomas.ach@ukbonn.de.
Abstract
BACKGROUND: For an understanding of the pathology of retinal diseases, direct comparisons of high-resolution in vivo retinal imaging and ex vivo histological preparations are desirable. MATERIAL AND METHODS: Multimodal in vivo and ex vivo imaging of a human donor eye with secondary alterations showing atrophic retina due to central retinal arterial occlusion. The subsequent correlation with the histological examination was carried out on identical tissue localizations. RESULTS: Appropriate custom-built retinal imaging devices facilitate in vivo and ex vivo correlations and the examination of human eye tissue and acquisition of retinal images, e.g. SD-OCT. The precise alignment of the tissue enables a histological analysis on identical sites. CONCLUSION: The direct correlation of clinical in vivo imaging with ex vivo imaging including histopathology can further enhance our understanding in the pathogenesis of retinal diseases; however, the proposed method is currently limited due to restricted availability of human donor tissue.
BACKGROUND: For an understanding of the pathology of retinal diseases, direct comparisons of high-resolution in vivo retinal imaging and ex vivo histological preparations are desirable. MATERIAL AND METHODS: Multimodal in vivo and ex vivo imaging of a human donor eye with secondary alterations showing atrophic retina due to central retinal arterial occlusion. The subsequent correlation with the histological examination was carried out on identical tissue localizations. RESULTS: Appropriate custom-built retinal imaging devices facilitate in vivo and ex vivo correlations and the examination of human eye tissue and acquisition of retinal images, e.g. SD-OCT. The precise alignment of the tissue enables a histological analysis on identical sites. CONCLUSION: The direct correlation of clinical in vivo imaging with ex vivo imaging including histopathology can further enhance our understanding in the pathogenesis of retinal diseases; however, the proposed method is currently limited due to restricted availability of human donor tissue.
Authors: Claudine E Pang; Jeffrey D Messinger; Emma C Zanzottera; K Bailey Freund; Christine A Curcio Journal: Ophthalmology Date: 2015-08-19 Impact factor: 12.079
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