Literature DB >> 3298686

Cloning, purification, and biochemical characterization of the pneumococcal bacteriophage Cp-1 lysin.

J L García, E García, A Arrarás, P García, C Ronda, R López.   

Abstract

Cp-1, a small virulent bacteriophage infecting Streptococcus pneumoniae, encodes its own lytic enzyme (CPL). A fragment of Cp-1 DNA containing the gene cpl coding for CPL was cloned and expressed in high amounts in Escherichia coli. CPL was purified to electrophoretic homogeneity by using affinity chromatography on choline-Sepharose (T. Briese and R. Hakenbeck, Eur. J. Biochem. 146:417-427, 1985), and the enzyme showing a Mr of 39,000 was characterized as a muramidase. This muramidase required for in vivo and in vitro activity the presence of choline in the teichoic acids of the pneumococcal cell walls. Free choline or lipoteichoic acid noncompetitively inhibited the activity of CPL.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3298686      PMCID： PMC255702          DOI： 10.1128/JVI.61.8.2573-2580.1987

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  38 in total

1.  Purification of the pneumococcal N-acetylmuramyl-L-alanine amidase to biochemical homogeneity.

Authors:  J V Höltje; A Tomasz
Journal:  J Biol Chem       Date:  1976-07-25       Impact factor: 5.157

2.  REGULATION OF THE TRANSFORMABILITY OF PHEUMOCOCCAL CULTURES BY MACROMOLECULAR CELL PRODUCTS.

Authors:  A TOMASZ; R D HOTCHKISS
Journal:  Proc Natl Acad Sci U S A       Date:  1964-03       Impact factor: 11.205

3.  Abnormal autolytic enzyme in a pneumococus with altered teichoic acid composition.

Authors:  A Tomasz; M Westphal
Journal:  Proc Natl Acad Sci U S A       Date:  1971-11       Impact factor: 11.205

4.  A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors:  H W Boyer; D Roulland-Dussoix
Journal:  J Mol Biol       Date:  1969-05-14       Impact factor: 5.469

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Pneumococcal Forssman antigen. A choline-containing lipoteichoic acid.

Authors:  E B Briles; A Tomasz
Journal:  J Biol Chem       Date:  1973-09-25       Impact factor: 5.157

7.  Lipoteichoic acid: a specific inhibitor of autolysin activity in Pneumococcus.

Authors:  J V Höltje; A Tomasz
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

8.  On the physiological functions of teichoic acids.

Authors:  A Tomasz; M Westphal; E B Briles; P Fletcher
Journal:  J Supramol Struct       Date:  1975

9.  Role of the pneumococcal autolysin (murein hydrolase) in the release of progeny bacteriophage and in the bacteriophage-induced lysis of the host cells.

Authors:  C Ronda-Lain; R Lopez; A Tapia; A Tomasz
Journal:  J Virol       Date:  1977-01       Impact factor: 5.103

10.  The chain length of the glycans in bacterial cell walls.

Authors:  J B Ward
Journal:  Biochem J       Date:  1973-06       Impact factor: 3.857
View more
  24 in total

1.  Purification and polar localization of pneumococcal LytB, a putative endo-beta-N-acetylglucosaminidase: the chain-dispersing murein hydrolase.

Authors:  Blanca De Las Rivas; José L García; Rubens López; Pedro García
Journal:  J Bacteriol       Date:  2002-09       Impact factor: 3.490

Review 2.  Bacteriophage lysis: mechanism and regulation.

Authors:  R Young
Journal:  Microbiol Rev       Date:  1992-09

3.  Cpl-7, a lysozyme encoded by a pneumococcal bacteriophage with a novel cell wall-binding motif.

Authors:  Noemí Bustamante; Nuria E Campillo; Ernesto García; Cristina Gallego; Benet Pera; Gregory P Diakun; José Luis Sáiz; Pedro García; J Fernando Díaz; Margarita Menéndez
Journal:  J Biol Chem       Date:  2010-08-18       Impact factor: 5.157

4.  Removal of group B streptococci colonizing the vagina and oropharynx of mice with a bacteriophage lytic enzyme.

Authors:  Qi Cheng; Daniel Nelson; Shiwei Zhu; Vincent A Fischetti
Journal:  Antimicrob Agents Chemother       Date:  2005-01       Impact factor: 5.191

5.  Pneumococcal LytA autolysin, a potent therapeutic agent in experimental peritonitis-sepsis caused by highly beta-lactam-resistant Streptococcus pneumoniae.

Authors:  Violeta Rodríguez-Cerrato; Pedro García; Lorena Huelves; Ernesto García; Gema Del Prado; Matilde Gracia; Carmen Ponte; Rubens López; Francisco Soriano
Journal:  Antimicrob Agents Chemother       Date:  2007-06-18       Impact factor: 5.191

6.  Identification of atypical strains of Streptococcus pneumoniae by a specific DNA probe.

Authors:  A Fenoll; J V Martinez-Suarez; R Muñoz; J Casal; J L Garcia
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1990-06       Impact factor: 3.267

7.  LysGH15, a novel bacteriophage lysin, protects a murine bacteremia model efficiently against lethal methicillin-resistant Staphylococcus aureus infection.

Authors:  Jingmin Gu; Wei Xu; Liancheng Lei; Jing Huang; Xin Feng; Changjiang Sun; Chongtao Du; Jing Zuo; Yang Li; Taofeng Du; Linxi Li; Wenyu Han
Journal:  J Clin Microbiol       Date:  2010-11-03       Impact factor: 5.948

8.  Chimeric phage-bacterial enzymes: a clue to the modular evolution of genes.

Authors:  E Díaz; R López; J L García
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

9.  Characterization of the pneumococcal bacteriophage HB-3 amidase: cloning and expression in Escherichia coli.

Authors:  A Romero; R Lopez; P Garcia
Journal:  J Virol       Date:  1990-01       Impact factor: 5.103

10.  Analysis of the complete nucleotide sequence and functional organization of the genome of Streptococcus pneumoniae bacteriophage Cp-1.

Authors:  A C Martín; R López; P García
Journal:  J Virol       Date:  1996-06       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.