Wen Wen1, Qiaoling Yao2, Yulan Chen1, Zhiqiang Li3, Xiaojing Sun4, Yu Li5, Junshi Zhang1, Zhulipiya Simayi1, Xinjuan Xu1. 1. Department of Hypertension, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China. 2. Department of Physiology, Preclinical Medicine Collage of Xinjiang Medical University, Urumqi 830054, China. 3. Clinical Medical Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China. 4. Department of Critical Medicine, the Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China. 5. Second Department of General Internal Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China.
Abstract
OBJECTIVE: To investigate the expression of transient receptor potential canonical channels (TRPCs) in the heart and kidney of rat model of obstructive sleep apnea hypopnea syndrome (OSAHS). METHODS: Eighteen male SD rats were randomly assigned to intermittent hypoxia (IH) group (n=9 ) and control group (n=9). In IH group, rats were placed in a chamber and exposed to intermittent hypoxia for 8h (10AM-6PM) daily. The expression of TRPC-related mRNA and protein in the heart and kidney tissue were detected by qRT-PCR and Western blotting, respectively. RESULTS: The mRNA expressions of TRPC3/TRPC4/TRPC5 in heart tissues of IH group were increased significantly compared with the control group (all P>0.05); while there were no significant differences in the mRNA expressions of TRPC1/TRPC3/TRPC4/TRPC5/TRPC6/TRPC7 in kidney tissue between two groups (all P<0.05). The mRNA expressions of TRPC4, TRPC5 and TRPC6 in kidney tissues of IH group were lower than that in heart tissues (all P<0.05). The mRNA expression of TRPC7 in kidney tissues of control group was significantly higher than that in heart tissues (P<0.05). The expression of TRPC5 protein in heart tissues of IH group was significantly higher than that in the control group (P<0.05); while there was no significant differences in the expression of TRPC5/TRPC6/TRPC7 protein in kidney tissue between two groups (all P>0.05). CONCLUSIONS: The IH rat model shows that TRPC5 channel is likely to be involved in the OSAHS induced pathophysiological changes in the myocardium and may become a target to prevent OSAHS related cardiac damage.
OBJECTIVE: To investigate the expression of transient receptor potential canonical channels (TRPCs) in the heart and kidney of rat model of obstructive sleep apnea hypopnea syndrome (OSAHS). METHODS: Eighteen male SD rats were randomly assigned to intermittent hypoxia (IH) group (n=9 ) and control group (n=9). In IH group, rats were placed in a chamber and exposed to intermittent hypoxia for 8h (10AM-6PM) daily. The expression of TRPC-related mRNA and protein in the heart and kidney tissue were detected by qRT-PCR and Western blotting, respectively. RESULTS: The mRNA expressions of TRPC3/TRPC4/TRPC5 in heart tissues of IH group were increased significantly compared with the control group (all P>0.05); while there were no significant differences in the mRNA expressions of TRPC1/TRPC3/TRPC4/TRPC5/TRPC6/TRPC7 in kidney tissue between two groups (all P<0.05). The mRNA expressions of TRPC4, TRPC5 and TRPC6 in kidney tissues of IH group were lower than that in heart tissues (all P<0.05). The mRNA expression of TRPC7 in kidney tissues of control group was significantly higher than that in heart tissues (P<0.05). The expression of TRPC5 protein in heart tissues of IH group was significantly higher than that in the control group (P<0.05); while there was no significant differences in the expression of TRPC5/TRPC6/TRPC7 protein in kidney tissue between two groups (all P>0.05). CONCLUSIONS: The IH rat model shows that TRPC5 channel is likely to be involved in the OSAHS induced pathophysiological changes in the myocardium and may become a target to prevent OSAHS related cardiac damage.
Authors: Georgios C Bompotis; Spyridon Deftereos; Christos Angelidis; Efthymios Choidis; Vasiliki Panagopoulou; Andreas Kaoukis; Vassilios P Vassilikos; Michael W Cleman; Georgios Giannopoulos Journal: Med Chem Date: 2016 Impact factor: 2.745
Authors: Suzy J Campbell; Susana V Nery; Rebecca Wardell; Catherine A D'Este; Darren J Gray; James S McCarthy; Rebecca J Traub; Ross M Andrews; Stacey Llewellyn; Andrew J Vallely; Gail M Williams; Archie C A Clements Journal: PLoS Negl Trop Dis Date: 2017-03-27