Literature DB >> 3298314

Anti-bovine antibody in human sera as a cause of nonspecificity in enzyme immunoassay.

T Dise, P A Brunell.   

Abstract

Much nonspecific activity in enzyme immunoassay is due to reactions between anti-bovine antibodies which are commonly present in human serum samples and residual calf serum in viral antigens. Calf serum is used in cultured cells which are used to produce viral antigens. Addition of bovine proteins to serum samples to be tested for viral antibodies reduces this activity.

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Year:  1987        PMID： 3298314      PMCID： PMC269121          DOI： 10.1128/jcm.25.6.987-990.1987

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  6 in total

1.  Enzyme-linked immunosorbent assay and radioimmunoassay in the serologic diagnosis of infectious diseases.

Authors:  H R Hill; J M Matsen
Journal:  J Infect Dis       Date:  1983-02       Impact factor: 5.226

2.  Enzyme-linked immunosorbent assay for susceptibility to varicella.

Authors:  Z Shehab; P A Brunell
Journal:  J Infect Dis       Date:  1983-09       Impact factor: 5.226

3.  T cell mediated polyclonal B cell activation induced by cell-bound fetal calf serum.

Authors:  J S Cowdery; A D Steinberg
Journal:  Int Arch Allergy Appl Immunol       Date:  1983

4.  ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes.

Authors:  B M Gray
Journal:  J Immunol Methods       Date:  1979       Impact factor: 2.303

5.  Effect of early immunization on antibody response to reimmunization with measles vaccine as demonstrated by enzyme-linked immunosorbent assay (ELISA).

Authors:  M D Murphy; P A Brunell; A W Lievens; Z M Shehab
Journal:  Pediatrics       Date:  1984-07       Impact factor: 7.124

6.  Absorption of protein and protein fragments in the developing intestine: role in immunologic/allergic reactions.

Authors:  W A Walker
Journal:  Pediatrics       Date:  1985-01       Impact factor: 7.124
  6 in total
  1 in total

1.  Establishment by enzyme-linked immunosorbent assay of seronegative range for herpes simplex virus and cytomegalovirus antibodies and evaluation of heterologous responses to live varicella vaccine.

Authors:  A K Gong; S V Lipton; P A Brunell
Journal:  J Clin Microbiol       Date:  1988-04       Impact factor: 5.948
  1 in total

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