Ciliu Zhang1, ChengLiang Zhang2, Yan Xu3, Chengjun Li4, Yong Cao5, Ping Li6. 1. Department of Pediatrics, Xiangya Hospital, Central South University Changsha, 410008, China. 2. Department of Cardiovascular Surgery, Xiangya Hospital, Central South University, 410008 Changsha, China. 3. Department of Sports Medicine, Xiangya Hospital, Central South University, 410008 Changsha, China; Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, 410008 Changsha, China. 4. Department of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, 410008 Changsha, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, 410008 Changsha, China. Electronic address: 443555063@qq.com. 5. Department of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, 410008 Changsha, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, 410008 Changsha, China. Electronic address: caoyong1912@163.com. 6. Department of Obstetrics, Xiangya Hospital, Central South University Changsha, 410008, China; Hunan Engineering Research Center of Early Life Development and Disease Prevention, Changsha, 410008, China. Electronic address: pingli19870927@163.com.
Abstract
BACKGROUND: Spinal cord injury (SCI) is a serious central nervous system condition with no effective clinal treatment. Recently, transplantation of bone marrow mesenchymal stem cells (MSCs) derived exosomes has been proposed as a potential treatment for SCI. However, whether exosomes have similar functions as transplanted human placenta-derived MSCs(hPMSCs) has remained unclear. METHODS: The hPMSCs-derived exosomes (hPMSCs-Exos) were extracted using a sequential centrifugation approach. Then, the effects of hPMSCs-Exos on angiogenesis were analysis both in vitro and in vivo. In addition, the sensory and locomotor functions of mice after SCI were also analyzed. RESULTS: The administration of hPMSCs-Exos promote the tube formation and migration of human umbilical vein endothelial cell (HUVECs). Furthermore, vessel numbers, vessel volume fraction and vessel connectivity in spinal cords significantly increased after exosomes were intrathecally injected in the SCI model. In addition, the locomotor and sensory function, also significantly improved in the exosome treatment group. CONCLUSIONS: The results of the present study demonstrated that hPMSCs-Exos have proangiogenic effects on endothelial cells and enhanced angiogenesis in SCI model. Thus, this treatment strategy demonstrates great potential for the treatment of SCI.
BACKGROUND:Spinal cord injury (SCI) is a serious central nervous system condition with no effective clinal treatment. Recently, transplantation of bone marrow mesenchymal stem cells (MSCs) derived exosomes has been proposed as a potential treatment for SCI. However, whether exosomes have similar functions as transplanted human placenta-derived MSCs(hPMSCs) has remained unclear. METHODS: The hPMSCs-derived exosomes (hPMSCs-Exos) were extracted using a sequential centrifugation approach. Then, the effects of hPMSCs-Exos on angiogenesis were analysis both in vitro and in vivo. In addition, the sensory and locomotor functions of mice after SCI were also analyzed. RESULTS: The administration of hPMSCs-Exos promote the tube formation and migration of human umbilical vein endothelial cell (HUVECs). Furthermore, vessel numbers, vessel volume fraction and vessel connectivity in spinal cords significantly increased after exosomes were intrathecally injected in the SCI model. In addition, the locomotor and sensory function, also significantly improved in the exosome treatment group. CONCLUSIONS: The results of the present study demonstrated that hPMSCs-Exos have proangiogenic effects on endothelial cells and enhanced angiogenesis in SCI model. Thus, this treatment strategy demonstrates great potential for the treatment of SCI.