Literature DB >> 3290843

Circular DNA of 3T6R50 double minute chromosomes.

A M van der Bliek1, C R Lincke, P Borst.   

Abstract

In pulsed field gradient gel electrophoresis (PFGE) the intact deproteinized circular DNA of Mycoplasma (800 kb) and Escherichia coli (4700 kb) remains trapped in the slot. We show here that gamma-irradiation of the DNA in agarose plugs is a convenient method to partially convert these circles into full-length linears, migrating with the expected mobility in PFGE. We have used this method to study the structure of Double Minute chromosomes (DMs) from the methotrexate (MTX)-resistant mouse cell line 3T6R50. Intact deproteinized DM DNA is immobile in these gels, but is converted into a single band of about 2500 kb by either gamma-irradiation, DNaseI in the presence of Mn2+, or restriction enzymes. We conclude that the DM DNA in 3T6R50 cells consists of a homogeneous population of 2500-kb circles.

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Year:  1988        PMID： 3290843      PMCID： PMC336700          DOI： 10.1093/nar/16.11.4841

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

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Authors:  A Bernards; J M Kooter; P A Michels; R M Moberts; P Borst
Journal:  Gene       Date:  1986       Impact factor: 3.688

2.  Mapping of VSG genes on large expression-site chromosomes of Trypanosoma brucei separated by pulsed-field gradient electrophoresis.

Authors:  P J Johnson; P Borst
Journal:  Gene       Date:  1986       Impact factor: 3.688

3.  Stable amplified DNA in drug-resistant Leishmania exists as extrachromosomal circles.

Authors:  E P Garvey; D V Santi
Journal:  Science       Date:  1986-08-01       Impact factor: 47.728

4.  Ultrastructural features of minute chromosomes in a methotrexate-resistant mouse 3T3 cell line.

Authors:  B A Hamkalo; P J Farnham; R Johnston; R T Schimke
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

5.  Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors:  G F Carle; M V Olson
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

6.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

7.  Isolation and expression of an altered mouse dihydrofolate reductase cDNA.

Authors:  C C Simonsen; A D Levinson
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205

8.  Relationship of amplified dihydrofolate reductase genes to double minute chromosomes in unstably resistant mouse fibroblast cell lines.

Authors:  P C Brown; S M Beverley; R T Schimke
Journal:  Mol Cell Biol       Date:  1981-12       Impact factor: 4.272

9.  Novel submicroscopic extrachromosomal elements containing amplified genes in human cells.

Authors:  B J Maurer; E Lai; B A Hamkalo; L Hood; G Attardi
Journal:  Nature       Date:  1987 Jun 4-10       Impact factor: 49.962

10.  An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.

Authors:  C L Smith; T Matsumoto; O Niwa; S Klco; J B Fan; M Yanagida; C R Cantor
Journal:  Nucleic Acids Res       Date:  1987-06-11       Impact factor: 16.971
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  19 in total

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Authors:  J C Ruiz; K H Choi; D D von Hoff; I B Roninson; G M Wahl
Journal:  Mol Cell Biol       Date:  1989-01       Impact factor: 4.272

2.  Replication timing control can be maintained in extrachromosomally amplified genes.

Authors:  S M Carroll; J Trotter; G M Wahl
Journal:  Mol Cell Biol       Date:  1991-09       Impact factor: 4.272

Review 3.  New approaches for physical mapping of small genomes.

Authors:  C L Smith; G Condemine
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490

4.  Chromosomal destabilization during gene amplification.

Authors:  J C Ruiz; G M Wahl
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

5.  DNA content and structure of (double) minutes of a methotrexate-resistant cell line.

Authors:  A P Jongsma; W A Duijndam; P Borst
Journal:  Histochemistry       Date:  1989

6.  Amplicon structure in multidrug-resistant murine cells: a nonrearranged region of genomic DNA corresponding to large circular DNA.

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Journal:  Mol Cell Biol       Date:  1992-03       Impact factor: 4.272

7.  Pulsed-field gel electrophoresis of circular DNA.

Authors:  J S Simske; S Scherer
Journal:  Nucleic Acids Res       Date:  1989-06-12       Impact factor: 16.971

8.  Different roles for nonhomologous end joining and homologous recombination following replication arrest in mammalian cells.

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Journal:  Mol Cell Biol       Date:  2002-08       Impact factor: 4.272

9.  Physical map and genome sequencing survey of Mycoplasma haemofelis (Haemobartonella felis).

Authors:  Linda M Berent; Joanne B Messick
Journal:  Infect Immun       Date:  2003-06       Impact factor: 3.441

10.  Frequent amplification of a short chain dehydrogenase gene as part of circular and linear amplicons in methotrexate resistant Leishmania.

Authors:  B Papadopoulou; G Roy; M Ouellette
Journal:  Nucleic Acids Res       Date:  1993-09-11       Impact factor: 16.971
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