Caidong Liu1, Haoyu Wang2, Chenbin Zhu1, Shukui Wang1. 1. Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China. 2. Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, China.
Abstract
BACKGROUND: Obstructive sleep apnea-hypopnea syndrome (OSAHS) is a common breathing disorder during sleep with potential lethality and multi-complications. Polysomnography (PSG) is now the golden standard for the diagnosis obstructive sleep apnea-hypopnea syndrome. However, PSG is expensive and time-consuming. Therefore, it is important to find inexpensive and convenient biomarkers for the diagnosis of OSAHS. OBJECTIVE: The present study aimed to explore the potential diagnostic value of HIF-1α for OSAHS and its clinical significance. METHODS: This study consisted of 368 patients admitted to the sleep laboratory. The patients were classified according to their apnea-hypopnea index (AHI) scores as OSA negative (AHI < 5), mild-moderate (AHI:5-30), and severe OSA (AHI > 30), and severe OSA treated with continuous positive airway pressure (CPAP). qRT-PCR was used to detect mRNA levels in the plasma; Pearson's correlation analysis was performed to analyze the correlation of HIF-1α mRNA level and the clinicopathological factors of OSAHS; ROC curve was constructed to evaluate the diagnostic value of HIF-1α mRNA. RESULTS: HIF-1α mRNA was significantly up-regulated in the plasma of OSAHS patients, especially patients with severe OSAHS. HIF-1α mRNA was positively correlated with the AHI and ODI but negatively correlated with the mean oxygen saturation in patients with OSAHS. Results of ROC curve showed that HIF-1α is a sensitive biomarker for the diagnosis of OSAHS, especially severe OSAHS. CONCLUSIONS: HIF-1α mRNA might be used as s a convenient and inexpensive method for triaging OSAHS patients PSG assessment in the hospital and evaluate the curative effect.
BACKGROUND: Obstructive sleep apnea-hypopnea syndrome (OSAHS) is a common breathing disorder during sleep with potential lethality and multi-complications. Polysomnography (PSG) is now the golden standard for the diagnosis obstructive sleep apnea-hypopnea syndrome. However, PSG is expensive and time-consuming. Therefore, it is important to find inexpensive and convenient biomarkers for the diagnosis of OSAHS. OBJECTIVE: The present study aimed to explore the potential diagnostic value of HIF-1α for OSAHS and its clinical significance. METHODS: This study consisted of 368 patients admitted to the sleep laboratory. The patients were classified according to their apnea-hypopnea index (AHI) scores as OSA negative (AHI < 5), mild-moderate (AHI:5-30), and severe OSA (AHI > 30), and severe OSA treated with continuous positive airway pressure (CPAP). qRT-PCR was used to detect mRNA levels in the plasma; Pearson's correlation analysis was performed to analyze the correlation of HIF-1α mRNA level and the clinicopathological factors of OSAHS; ROC curve was constructed to evaluate the diagnostic value of HIF-1α mRNA. RESULTS: HIF-1α mRNA was significantly up-regulated in the plasma of OSAHS patients, especially patients with severe OSAHS. HIF-1α mRNA was positively correlated with the AHI and ODI but negatively correlated with the mean oxygen saturation in patients with OSAHS. Results of ROC curve showed that HIF-1α is a sensitive biomarker for the diagnosis of OSAHS, especially severe OSAHS. CONCLUSIONS: HIF-1α mRNA might be used as s a convenient and inexpensive method for triaging OSAHS patients PSG assessment in the hospital and evaluate the curative effect.
Authors: Richard B Berry; Rohit Budhiraja; Daniel J Gottlieb; David Gozal; Conrad Iber; Vishesh K Kapur; Carole L Marcus; Reena Mehra; Sairam Parthasarathy; Stuart F Quan; Susan Redline; Kingman P Strohl; Sally L Davidson Ward; Michelle M Tangredi Journal: J Clin Sleep Med Date: 2012-10-15 Impact factor: 4.062
Authors: Wesley Elon Fleming; Aliya Ferouz-Colborn; Michael K Samoszuk; Armaghan Azad; Jiuliu Lu; John S Riley; Amabelle B Cruz; Susann Podolak; Doni J Clark; Kurtis R Bray; Paula C Southwick Journal: Clin Biochem Date: 2016-05-13 Impact factor: 3.281