Electron tomography and immunogold labeling of plant cells.

Electron microscopy enables the imaging of organelles and macromolecular complexes within cells at nanometer scale resolution. Electron tomography of biological samples, either in vitrified ice or fixed and embedded in resin, provides three-dimensional structural information of relatively small volumes (a few cubic microns) of cells at axial resolutions of 1-7nm. This chapter discusses approaches for plant sample preparation by high-pressure freezing/freeze-substitution and resin-embedding for electron tomography and immunogold labeling using transmission electron microscopy.