| Literature DB >> 3288688 |
Abstract
DNA-dependent RNA polymerase from Escherichia coli was purified further by elution through heparin-Sepharose CL-6B column after the enzyme was obtained, partially purified, using Burgess and Jendrisak's method [(1975)Biochemistry 14, 4634] The total yield of the pure protein was 10 mg from 50 g of E.coli cells. The method was found to be very reproducible and convenient. The enzyme preparation had 60% active molecules and the elongation rate of RNA synthesis by this enzyme was measured to be 11 bases/s over delta D111 T7 DNA.Entities:
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Year: 1988 PMID： 3288688 DOI： 10.1016/0165-022x(88)90010-3
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X