| Literature DB >> 32883834 |
Wei Wang1,2, Chi-Kuo Hu3, An Zeng1, Dana Alegre1, Deqing Hu1, Kirsten Gotting1, Augusto Ortega Granillo1, Yongfu Wang1, Sofia Robb1, Robert Schnittker1, Shasha Zhang1, Dillon Alegre1, Hua Li1, Eric Ross1,2, Ning Zhang1, Anne Brunet3,4, Alejandro Sánchez Alvarado5,2.
Abstract
Vertebrates vary in their ability to regenerate, and the genetic mechanisms underlying such disparity remain elusive. Comparative epigenomic profiling and single-cell sequencing of two related teleost fish uncovered species-specific and evolutionarily conserved genomic responses to regeneration. The conserved response revealed several regeneration-responsive enhancers (RREs), including an element upstream to inhibin beta A (inhba), a known effector of vertebrate regeneration. This element activated expression in regenerating transgenic fish, and its genomic deletion perturbed caudal fin regeneration and abrogated cardiac regeneration altogether. The enhancer is present in mammals, shares functionally essential activator protein 1 (AP-1)-binding motifs, and responds to injury, but it cannot rescue regeneration in fish. This work suggests that changes in AP-1-enriched RREs are likely a crucial source of loss of regenerative capacities in vertebrates.Entities:
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Year: 2020 PMID： 32883834 PMCID： PMC9479427 DOI： 10.1126/science.aaz3090
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 63.714