Pablo Moreno-Acosta1,2, MÓnica Molano3, Nicolas Morales2, Jinneth Acosta4, Cristian GonzÁlez-Prieto5, Diana Mayorga6, Lina Buitrago7, Oscar Gamboa7, Juan Carlos MejÍa8, July Castro8, Alfredo Romero-Rojas8, Sophie Espenel9, Gerald L Murray3,10,11, Suzanne M Garland3,10,11, Alexis Vallard9, Nicolas MagnÉ9. 1. Research Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombia pmoreno@cancer.gov.co. 2. Research Group in Cancer Biology, National Cancer Institute, Bogotá, Colombia. 3. Centre Women's Infectious Diseases Research, The Royal Women's Hospital, Melbourne, Australia. 4. Pathology Group, National University of Colombia, Bogotá, Colombia. 5. Statistics Department, National University of Colombia, Bogotá, Colombia. 6. Research Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombia. 7. Unit of Analysis, National Cancer Institute, Bogotá, Colombia. 8. Group of Pathology Oncology, National Cancer Institute, Bogotá, Colombia. 9. Department of Radiation Oncology, Institut de Cancérologie de la Loire-Lucien Neuwirth, Saint-Priest en Jarez, France. 10. Department of Obstetrics and Gynecology, University of Melbourne, Parkville, VIC, Australia. 11. Murdoch Children's Research Institute, Parkville, VIC, Australia.
Abstract
BACKGROUND: Few studies have analyzed the association between human telomerase reverse transcriptase (hTERT) protein expression (nuclear and cytoplasmic localization), hTERT methylation status, and human papillomavirus (HPV) genotype infection in cervical cancer. PATIENTS AND METHODS: One hundred seventy-three patients with cervical cancer were analyzed. hTERT protein expression was detected by immunohistochemistry. hTERT DNA methylation analysis was performed using a PCR-RLB-hTERT assay, targeting two regions of the hTERT promoter. Type specific HPV infection was detected by using GP5+/GP6+PCR-RLB. RESULTS: hTERT protein expression was found in both cytoplasm and nucleus (78.0% of the samples showed a cytoplasmic localization and 79.8% had a nuclear localization). A statistically significant association was found between alpha 9 and 7 HPV species with a non-methylation pattern of the hTERT promoter and between these species and high expression of hTERT protein with nuclear localization. CONCLUSION: hTERT protein is found in both the nucleus and cytoplasm of patients with cervical cancer and confirm the relationship between the non-methylated status of hTERT promoter and some HPV species as well as the relationship between these species and hTERT protein expression. Copyright
BACKGROUND: Few studies have analyzed the association between human telomerase reverse transcriptase (hTERT) protein expression (nuclear and cytoplasmic localization), hTERT methylation status, and human papillomavirus (HPV) genotype infection in cervical cancer. PATIENTS AND METHODS: One hundred seventy-three patients with cervical cancer were analyzed. hTERT protein expression was detected by immunohistochemistry. hTERT DNA methylation analysis was performed using a PCR-RLB-hTERT assay, targeting two regions of the hTERT promoter. Type specific HPV infection was detected by using GP5+/GP6+PCR-RLB. RESULTS:hTERT protein expression was found in both cytoplasm and nucleus (78.0% of the samples showed a cytoplasmic localization and 79.8% had a nuclear localization). A statistically significant association was found between alpha 9 and 7 HPV species with a non-methylation pattern of the hTERT promoter and between these species and high expression of hTERT protein with nuclear localization. CONCLUSION:hTERT protein is found in both the nucleus and cytoplasm of patients with cervical cancer and confirm the relationship between the non-methylated status of hTERT promoter and some HPV species as well as the relationship between these species and hTERT protein expression. Copyright
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