Satoshi Kutsuna1, Kayoko Hayakawa2, Kazuhisa Mezaki3, Kei Yamamoto2, Norio Ohmagari2. 1. Disease Control and Prevention Center, National Center for Global Health and Medicine, Japan. Electronic address: sonare.since1192@gmail.com. 2. Disease Control and Prevention Center, National Center for Global Health and Medicine, Japan. 3. Microbiology Laboratory, National Center for Global Health and Medicine, Japan.
Abstract
INTRODUCTION: Traveler's diarrhea (TD) is the most common illness among people traveling from resource-rich regions to resource-limited regions, although the precise microbial etiology is unclear in many cases. METHODS: Stool specimens were prospectively collected from 106 consecutive patients with TD and 16 healthy controls without TD, and were tested using both the FilmArray gastrointestinal panel (BioFire Diagnostics) and conventional stool cultures. RESULTS: The 106 patients had traveled to Southeast Asia (55 cases), South Asia (22 cases), Africa (11 cases), and East Asia (7 cases). Among the 106 specimens, 95 specimens (89.6%) were positive for pathogens during the FilmArray testing. The FilmArray testing also identified multiple pathogens in 75.8% of the specimens from positive cases. Conventional stool cultures only detected pathogens in 23.6% of the specimens. CONCLUSION: The FilmArray gastrointestinal panel significantly improved the detection of enteropathogens and allowed for a rapid assessment of the TD's etiology. In addition, conventional stool cultures are likely to underestimate co-infections with multiple infectious pathogens.
INTRODUCTION: Traveler's diarrhea (TD) is the most common illness among people traveling from resource-rich regions to resource-limited regions, although the precise microbial etiology is unclear in many cases. METHODS: Stool specimens were prospectively collected from 106 consecutive patients with TD and 16 healthy controls without TD, and were tested using both the FilmArray gastrointestinal panel (BioFire Diagnostics) and conventional stool cultures. RESULTS: The 106 patients had traveled to Southeast Asia (55 cases), South Asia (22 cases), Africa (11 cases), and East Asia (7 cases). Among the 106 specimens, 95 specimens (89.6%) were positive for pathogens during the FilmArray testing. The FilmArray testing also identified multiple pathogens in 75.8% of the specimens from positive cases. Conventional stool cultures only detected pathogens in 23.6% of the specimens. CONCLUSION: The FilmArray gastrointestinal panel significantly improved the detection of enteropathogens and allowed for a rapid assessment of the TD's etiology. In addition, conventional stool cultures are likely to underestimate co-infections with multiple infectious pathogens.
Authors: Michele D Tisdale; David R Tribble; Indrani Mitra; Kalyani Telu; Huai-Ching Kuo; Jamie A Fraser; Jie Liu; Eric R Houpt; Mark S Riddle; Drake H Tilley; Anjali N Kunz; Heather C Yun; Charla C Geist; Tahaniyat Lalani Journal: J Travel Med Date: 2022-01-17 Impact factor: 39.194