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Literature DB >> 3283107

Export of hybrid proteins FhuA'-'LacZ and FhuA'-'PhoA to the cell envelope of Escherichia coli K-12.

Abstract

The fhuA gene of Escherichia coli K-12 encodes an outer membrane protein that acts as the ferrichrome-iron(III) receptor. To determine the export signals and sorting information within FhuA, gene fusions of fhuA'-'lacZ and fhuA'-'phoA were constructed. Although a FhuA'-'LacZ hybrid protein was detected in the Triton X-100-insoluble fraction of the cell envelope, direct immunoelectron microscopic observation showed that this protein remained in the cytoplasm. FhuA'-'PhoA hybrid proteins were all exported across the cytoplasmic membrane. Those hybrids containing up to 88 amino acids of FhuA (FhuA88) fused to PhoA were released along with other periplasmic proteins. Hybrids containing 180 or more amino acids of FhuA (FhuA180) fused to PhoA were associated with the outer membrane. It is proposed that some information inherent in the sequences between FhuA88 and FhuA180 confers stable association with the outer membrane.

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Year: 1988 PMID： 3283107 PMCID： PMC211117 DOI： 10.1128/jb.170.5.2267-2275.1988

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

28 in total

1. Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.

Authors: P J Bassford; T J Silhavy; J R Beckwith
Journal: J Bacteriol Date: 1979-07 Impact factor: 3.490

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

Review 3. Empirical predictions of protein conformation.

Authors: P Y Chou; G D Fasman
Journal: Annu Rev Biochem Date: 1978 Impact factor: 23.643

4. Sequence information within the lamB genes in required for proper routing of the bacteriophage lambda receptor protein to the outer membrane of Escherichia coli K-12.

Authors: M N Hall; M Schwartz; T J Silhavy
Journal: J Mol Biol Date: 1982-03-25 Impact factor: 5.469

9. Characterization of Escherichia coli expressing an Lpp'OmpA(46-159)-PhoA fusion protein localized in the outer membrane.

Authors: C Stathopoulos; G Georgiou; C F Earhart
Journal: Appl Microbiol Biotechnol Date: 1996-03 Impact factor: 4.813

10. Analysis of the structure and subcellular location of filamentous phage pIV.

Authors: M Russel; B Kaźmierczak
Journal: J Bacteriol Date: 1993-07 Impact factor: 3.490

Export of hybrid proteins FhuA'-'LacZ and FhuA'-'PhoA to the cell envelope of Escherichia coli K-12.

1. Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Review 3. Empirical predictions of protein conformation.

4. Sequence information within the lamB genes in required for proper routing of the bacteriophage lambda receptor protein to the outer membrane of Escherichia coli K-12.

5. A signal sequence is not sufficient to lead beta-galactosidase out of the cytoplasm.

Review 6. Mechanism of incorporation of cell envelope proteins in Escherichia coli.

7. Sequence analysis of mutations that prevent export of lambda receptor, an Escherichia coli outer membrane protein.

8. Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli.

9. Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.

10. Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant.

1. Internal deletions in the FhuA receptor of Escherichia coli K-12 define domains of ligand interactions.

2. Deletions or duplications in the BtuB protein affect its level in the outer membrane of Escherichia coli.

Review 3. Alkaline phosphatase fusions: sensors of subcellular location.

4. Genetic insertion and exposure of a reporter epitope in the ferrichrome-iron receptor of Escherichia coli K-12.

5. Topological analysis of the Escherichia coli ferrichrome-iron receptor by using monoclonal antibodies.

6. Sequences of the Escherichia coli BtuB protein essential for its insertion and function in the outer membrane.

7. Insertion mutagenesis of the gene encoding the ferrichrome-iron receptor of Escherichia coli K-12.

8. Export of FepA::PhoA fusion proteins to the outer membrane of Escherichia coli K-12.

9. Characterization of Escherichia coli expressing an Lpp'OmpA(46-159)-PhoA fusion protein localized in the outer membrane.

10. Analysis of the structure and subcellular location of filamentous phage pIV.