Effect of stent placement on stone recurrence and post-procedural cholangitis after endoscopic removal of common bile duct stones.

BACKGROUND/AIMS: After endoscopic treatment of common bile duct (CBD) stones, recurrence of choledocholithiasis due to small stone fragments and post-endoscopic retrograde cholangiopancreatography (post-ERCP) cholangitis can occur. We determined the effect of biliary stenting after removal of CBD stones on the recurrence of CBD stones and the incidence of post-ERCP cholangitis.
METHODS: We performed a retrospective single-center study involving 483 patients who underwent ERCP for the removal of CBD stones. The patients were classified into two groups according to their biliary stenting status. The primary outcome was the rate of CBD stone recurrence and the secondary outcome was the incidence of post-ERCP cholangitis.
RESULTS: Among the 483 patients, 219 and 264 did and did not receive a biliary stent after CBD stone removal, respectively. The incidence of stone recurrence was 15.5% and 7.6% in the non-stenting and stenting groups (p = 0.006), respectively, while the incidence of post-ERCP cholangitis was 4.6% and 2.7% (p = 0.256). In a multivariate analysis, biliary stenting significantly reduced the stone recurrence rate (odds ratio, 0.30; p = 0.004).
CONCLUSION: Biliary stenting after the removal of CBD stones reduces the stone recurrence rate and assisted recovery. For patients with large and multiple stones who undergo lithotripsy, preventive biliary stent insertion can reduce the rate of stone recurrence.

INTRODUCTION

Common bile duct (CBD) stones are frequently encountered in clinical practice. Choledocholithiasis is the most frequent cause of acute cholangitis and can result in significant morbidity and possibly mortality if untreated [1-3]. CBD stones cause biliary obstruction, which impedes bile flow and leads to cholangitis; the resulting elevated bile-duct pressure facilitates the translocation of bacteria or endotoxins into the vascular system. These mechanisms explain the link between CBD stones and the development of acute cholangitis [2,3].

The standard treatment for CBD stones is endoscopic retrograde cholangiopancreatography (ERCP) with endoscopic sphincterotomy (EST) and/or endoscopic papillary balloon dilatation (EPBD), which is minimally invasive and effective [1,4,5]. The success rate for endoscopic stone removal is 80% to 100% and the complication rate is 0% to 25% [6-9]. However, the recurrence of CBD stones and the development of cholangitis due to recurrent stones are challenging clinical issues. The rate of stone recurrence after endoscopic removal is reportedly 4% to 24% [10-13] and recurrence can be caused by several factors. The presence of large and multiple CBD stones after the first treatment and small stone fragments generated by lithotripsy are risk factors for recurrence [14]. Stone microcrystals may be overlooked during the final cholangiography immediately after the removal of CBD stones, potentially leading to stone recurrence and cholangitis.

To prevent stone recurrence, follow-up cholangiography using a nasobiliary catheter may be considered, but is inconvenient, tedious, and requires hospitalization [14,15]. The risk of residual CBD stones and can be reduced by irrigating the bile duct with saline, but irrigation does not reduce the risk of post-ERCP cholangitis significantly [16]. A large amount of saline irrigation may increase CBD pressure and cause ascending cholangitis with worsening of the patient’s clinical status. Methods for preventing the recurrence of CBD stones are lacking. We investigated the effect of biliary stenting after the endoscopic treatment of CBD stones on stone recurrence and post-procedural cholangitis.

METHODS

Study design and population

The study was a single-center retrospective study performed at Konkuk Medical Center in Seoul, Korea from January 2011 to December 2018. The study enrolled 483 patients who had undergone endoscopic CBD stone removal by ERCP. The enrolled patients were 18 to 90 years of age with no history of EST. The patient selection process is shown in Fig. 1. The inclusion criteria were as follows: (1) age over 18 years, (2) CBD stone size < 50 mm, and (3) naïve papilla. The exclusion criteria were as follows: (1) patients who had undergone hepatobiliary surgery or EST, (2) bleeding tendency or the use of anticoagulants or antiplatelet agents until 2 days prior to surgery, (3) CBD stones > 50 mm, (4) < 18 or > 90 years of age, (5) intrahepatic duct (IHD) stones, (6) failed selective cannulation, and (7) pregnancy (Fig. 1). This study was conducted in accordance with the Declaration of Helsinki and was approved by the Institutional Review Board for Human Research at Konkuk University Medical Center (KUH1010993). Written informed consent by the patients was waived due to a retrospective nature of our study.

Figure 1.

Algorithm for patient selection. CBD, common bile duct; EST, endoscopic sphincterotomy; IHD, intrahepatic duct.

Methods

The data for the included patients were analyzed retrospectively. The patients’ demographic and clinical variables, including age, gender, cholecystectomy state, hospital stay, and ERCP method and findings (i.e., number of CBD stones, periampullary diverticulum, sphincter management, and stone removal methods), were evaluated. A periampullary diverticulum was defined as a diverticulum 2 to 3 cm from the ampulla that did not encompass the ampulla [17].

Endoscopic retrograde cholangiography was performed using a side-view endoscope (JF-260, TJF-240, or TJF 260, Olympus Optical Co., Tokyo, Japan). The procedures were performed by three experienced endoscopists (> 500 ERCP cases). After a diagnostic cholangiogram and wire-guided cannulation, EST was conducted using a pull-type sphincterotome. For patients with an altered anatomy (periampullary diverticulum or Billroth II gastrojejunostomy) and for the removal of large stones (> 10 mm diameter) or ≥ 5 bile duct stones, a papillary balloon dilater (CRE balloon, Boston Scientific Microinvasive, Cork, Ireland) was passed over the guidewire and positioned at the middle of the balloon across the orifice of the ampulla. The diameter of the papillary balloon dilator was determined based on the size of the stones and the diameter of the bile duct. Under endoscopic and fluoroscopic guidance, the balloon was gradually filled with diluted contrast medium to achieve maximum expansion within 30 seconds. After EPBD, the biliary stones were extracted using a basket or retrieval balloon catheter with or without mechanical lithotripsy. To avoid potential stone fragments, balloon sweeping was performed using the retrieval balloon catheter and a balloon-occluded cholangiogram was obtained to confirm complete stone removal. After the procedure, none of the patients required endoscopic nasobiliary drainage (ENBD) in the non-stenting group. Liver function tests and vital sign monitoring were performed and the severity of cholangitis was evaluated at admission. For patients with severe cholangitis, ≥ 3 stones, periampullary diverticulum, or papillary edema on the cholangiogram after stone removal and lack of contrast medium drainage to the duodenum, the procedure was completed after the insertion of a 7-Fr plastic biliary stent (double pigtail biliary stent, Wilson Cook Medical Inc., Bloomington, IN, USA). Severe cholangitis was defined as follows: (1) patient had a cholangitis symptom with a high fever before the procedure regardless of the liver enzyme values, or (2) liver enzyme values were greater than three times the upper normal limit, or (3) patient had a dysfunction in one or more organs. Vital signs were measured at 8-hour intervals after the procedure and liver function tests were performed before and at 24 and 48 hours after the procedure. Liver function tests were performed 2 and 4 weeks after discharge. The biliary stents were removed within one month when liver function normalized.

Statistical analysis

Statistical analysis was performed using SPSS software for Windows version 17.0 (SPSS Inc., Chicago, IL, USA). The chi-squared test and Student’s t test were performed to identify significant differences in the baseline characteristics, rate of recurrence of CBD stones, post-procedural cholangitis, procedure duration, and rate of complications. To identify factors independently associated with the recurrence of CBD stones, multivariate regression analysis was performed using significantly or clinically associated factors identified in univariate analyses. A value of p < 0.05 was considered indicative of statistical significance.

Outcomes

The primary outcome was stone recurrence, defined as the development of cholangitis or the detection of stones on radiologic images during follow-up 90 days after the complete removal of CBD stones. The secondary outcomes were changes in liver enzyme levels before and after the procedure, post-ERCP cholangitis immediately after the procedure, and procedure-related complications (classified and graded according to the consensus guidelines with some modifications [18]). Post-ERCP cholangitis was defined as a temperature greater than 38°C for 24 to 48 hours after endoscopic treatment that was judged to have a biliary cause, with no other infections, preoperative fever [19], or deterioration of cholangitis for patients who had cholangitis prior to the ERCP.

RESULTS

Demographic and clinical characteristics

We analyzed the data for 565 patients with CBD stones diagnosed by ERCP. Among them, 82 patients were excluded due to a history of EST (n = 22) or abdominal surgery (n = 37), use of antiplatelet agents (n = 5), stones > 50 mm (n = 1), age > 90 years (n = 15), and IHD stones (n = 2). The patients were classified into a non-stenting group (n = 219) or a stenting group (n = 264). Fig. 1 shows the baseline characteristics of the patients with choledocholithiasis. The mean age of the non-stenting group was not significantly lower than that of the stenting group. In the stenting group, EPBD was performed more frequently (47.9% vs. 61.4%, respectively; p = 0.003), the stone size was larger (p = 0.031), and the incidence of multiple stones was higher (33.3% vs. 46.6%; respectively, p = 0.020) than in the non-stenting group. The total duration of hospital stay was shorter in the stenting group (mean 8.1 days vs. 8.3 days, respectively; p = 0.726). The mean total procedure time was 19.2 minutes in the stenting group and 14.7 minutes in the non-stenting group (p = 0.001). Endoscopic mechanical lithotripsy (EML) was performed more frequently in the stenting group than in the non-stenting group (15.9% vs. 5.0%, respectively; p = 0.001) (Table 1). In the stenting group, the stent was removed after an average of 33 days (interquartile range [IQR], 17 to 54). The median follow-up duration was 101 days (IQR, 35 to 414) in the non-stenting group and 108 days (IQR, 33 to 486) in the stenting group. None of the fatalities were related to the procedure or occurred during hospitalization.

Table 1.

Baseline characteristics and ERCP findings

Characteristic	Non-stenting (n = 219)	Stenting (n = 264)	p value
Age, yr	61.4 ± 17.2	63.8 ± 15.3	0.105
Sex			0.551
Male	121 (55.3)	153 (58.0)
Female	98 (44.7)	111 (42.0)
Cholecystectomy	144 (65.8)	192 (72.7)	0.097
Gallstone[a]	136 (62.1)	173 (65.5)	0.434
Hospitalization	8.3 ± 5.5	8.1 ± 4.5	0.726
ERCP method			0.003
EST only	114 (52.1)	102 (38.6)
EST + EPLBD	105 (47.9)	162 (61.4)
PAD	23 (10.5)	24 (9.1)	0.587
Multiple CBD stones	74 (33.8)	123 (46.6)	0.020
Largest stone size, mm	14.5 ± 7.0	16.1 ± 8.0	0.031
Procedure time, min	14.7 ± 7.6	19.2 ± 9.2	< 0.001
EML	11 (5.0)	42 (15.9)	< 0.001

Values are presented as mean ± SD or number (%).

ERCP, endoscopic retrograde cholangiopancreatography; EST, endoscopic sphincterotomy; EPLBD, endoscopic papillary large balloon dilatation; PAD, periampullary diverticulum; CBD, common bile duct; EML, endoscopic mechanical lithotripsy.

Stone in the gallbladder in situ.

Stones recurred in 54 of the 483 patients (11.2%): 20 of 264 patients (7.6%) in the stenting group and 34 of 219 (15.5%) in the non-stenting group (p = 0.006) (Fig. 2). Post-procedural cholangitis developed in 17 of the 483 patients, including 10 of 219 patients (4.6%) in the non-stenting group and seven of 264 (2.7%) in the stenting group (p = 0.256).

Figure 2.

Primary outcomes. Stones recurred in 34 of 185 patients (15.5%) in the non-stenting group and 20 of 264 (7.6%) in the stenting group (p = 0.006).

The initial aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were higher in the biliary stenting group than in the non-stenting group. However, at the 24-hour follow-up, the AST level was significantly lower in the stenting group than in the non-stenting group, with a decrease of more than 50% (Table 2). The decreases in the mean AST and ALT levels were greater in the stenting group. Further, the decrease in the AST level at 24 hours was significantly greater in the stenting group (mean ± standard deviation, 94.6 ± 89.4 IU/L) than in the non-stenting group (125.9 ± 166.6 IU/L, p = 0.010).

Table 2.

Secondary outcomes

Variable	Non-stenting (n = 219)	Stenting (n = 264)	p value
Post-ERCP cholangitis	10 (4.6)	7 (2.7)	0.256
AST
Initial, IU/L	172.4 ± 202.8	203.6 ± 244.2	0.132
50% decrease after 24 hr	62 (28.3)	107 (40.8)	0.004
50% decrease after 48 hr	98 (49.5)	129 (57.1)	0.118
ALT
Initial, IU/L	201.7 ± 212.3	225.1 ± 216.7	0.233
50% decrease after 24 hr	21 (9.7)	21 (8.0)	0.505
50% decrease after 48 hr	71 (37.0)	83 (35.8)	0.798
ALP
Initial, IU/L	192.7 ± 137.8	195.0 ± 119.0	0.850
50% decrease after 24 hr	0	0
50% decrease after 48 hr	40 (21.4)	52 (22.2)	0.837
Total bilirubin
Initial, mg/dL	4.4 ± 27.8	3.5 ± 3.9	0.591
50% decrease after 24 hr	44 (20.1)	68 (26.1)	0.124
50% decrease after 48 hr	69 (36.9)	108 (46.2)	0.056

Values are presented as number (%) or mean ± SD.

ERCP, endoscopic retrograde cholangiopancreatography; AST, aspartate aminotransferase; ALT, alanine aminotransaminase; ALP, alkaline phosphatase.

In univariate analyses, age (p = 0.007), biliary stenting (p = 0.007), gallstone (p = 0.025), EML (p = 0.007), total procedure duration (p = 0.019), and stone size (p = 0.003) were associated with stone recurrence. Among these factors, biliary stenting after CBD stone removal was found to be independently predictive of stone recurrence in multivariate analysis (Table 3).

Table 3.

Association of stone recurrence with clinical factors

Factor	Univariate analysis		Multivariate analysis
	OR (95% CI)	p value	OR (95% CI)	p value
Age	1.03 (1.01–1.05)	0.007	1.02 (1.00–1.04)	0.199
Stent	0.45 (0.25–0.80)	0.007	0.30 (0.14–0.69)	0.004
Cholecystectomy	0.65 (0.36–1.17)	0.154
Gallstone[a]	1.92 (1.09–3.39)	0.025	0.63 (0.31–1.28)	0.202
ERCP method[b]	0.70 (0.39–1.25)	0.230
EML	0.37 (0.18–0.76)	0.007	2.64 (1.06–6.56)	0.037
Procedure time	1.04 (1.01–1.07)	0.019	1.04 (1.00–1.08)	0.073
Stone size	1.06 (1.02–1.10)	0.003	1.03 (0.99–1.09)	0.174
PAD	0.82 (0.33–2.03)	0.662	0.91 (0.31-2.64)	0.860
Multiple stones	5.04 (0.8-031.80)	0.085	1.52 (0.75-3.10)	0.248

OR, odds ratio; CI, confidence interval; ERCP, endoscopic retrograde cholangiopancreatography; EML, endoscopic mechanical lithotripsy; PAD, periampullary diverticulum.

Stone in the gallbladder in situ.

Difference between endoscopic sphincterotomy (EST) only and EST + endoscopic papillary large balloon dilatation.

The ERCP-related complications were as follows. Bleeding occurred in four patients (1.5%) in the stenting group and four (1.8%) in the non-stenting group. Post-ERCP pancreatitis occurred in 18 patients (6.8%) in the stenting group and 12 (5.5%) in the non-stenting group (Table 4). There was no significant difference in the complication rate between the two groups and there was no biliary stent-related adverse event in the stenting group.

Table 4.

Procedure-related complications

Side effect	Non-stenting (n = 219)	Stenting (n = 264)	p value
Bleeding	4 (1.8)	4 (1.5)	0.790
Perforation	0	0	NA
Post-ERCP pancreatitis	12 (5.5)	18 (6.8)	0.544

ERCP, Endoscopic retrograde cholangiopancreatography; NA, not applicable.

DISCUSSION

Although the success rate of endoscopic treatment for CBD stones is improving, CBD stone recurrence remains a major concern. In this study, we evaluated the effect of biliary stenting on stone recurrence and found that the insertion of a stent into the CBD was associated with a reduction in the rate of stone recurrence.

Intraductal ultrasound (IDUS) provides real-time and high-quality cross-sectional images, enabling the detection of remnant stones. The rate of residual stones is 33% to 40% [20,21] and Tsuchiya et al. [21] reported a CBD stone recurrence rate of 3.4%, determined with IDUS. However, IDUS is costly, the probe is fragile, and the efficacy is highly operator-dependent [22]. Additionally, after stone removal with EST, distinguishing between residual stone and air in the biliary tract is problematic. Ursodeoxycholic acid (UDCA), a hydrophilic dihydroxy bile acid, prevents stone recurrence by improving cholestasis and reducing lithogenicity. A multicenter randomized trial demonstrated that UDCA treatment prevents stone recurrence [23]. However, bile-acid dissolution by UDCA cannot be applied to pigmented or mixed cholesterol stones. ENBD can be monitored easily and is a quick and simple method for biliary decompression. However, patients that undergo ENBD treatment will be uncomfortable and at risk of pulling out the transnasal tube. Endoscopic retrograde biliary drainage is an internal drainage procedure that is as effective as ENBD and causes less discomfort to patients [14,15]. Ahn et al. [16] reported that preventive saline irrigation of the bile duct (PSIB) after stone removal reduces the risk of residual CBD stones from 22.7% to 6.8%. However, PSIB after stone extraction can result in the migration of stone fragments upward into the intrahepatic bile ducts, where they can act as nidi for subsequent bile duct stones. Moreover, saline irrigation of the bile ducts under pressure may cause ascending cholangitis with worsening of the patient’s clinical status.

We investigated the effect of biliary stenting on the recurrence rate of CBD stones. Large calculi (≥ 15 mm diameter) and multiple stones are risk factors for recurrence [14]. EML is typically used to remove difficult stones but can leave small fragments. Additionally, after EST or EPBD, temporal edema due to stimulation of the papilla orifice by basket movement can cause incomplete drainage of bile acids, aggravation of post-ERCP cholangitis, or newly developed cholangitis and remnant fragments in the CBD. According to the guidelines, short-term use of a biliary stent may be associated with a decrease in the size and number of stones, and transient biliary stenting is recommended for the management of difficult ductal stones [5,24]. However, the effect of preventing recurrence after complete removal of stones has not been investigated. In the present study, the rate of CBD stone recurrence was lower in the stenting group despite the larger stone size and the higher frequency of EML. In other words, stent insertion may prevent stone recurrence in the long term. Also, the incidence of post-ERCP cholangitis was associated with reducing by biliary stenting, likely by promoting the passage of bile acids, alleviating edema, preventing inflammation, and inducing the release of stone fragments, thus preventing stone recurrence. There was no significant difference in post-procedural cholangitis between the two groups, possibly due to the small number of patients.

The effect of elevated serum AST and ALT levels in patients with biliary obstruction due to CBD stones is unclear. The serum AST and ALT levels increased by 28% and 88%, respectively, in patients with biliary obstruction [25]. These changes are caused by liver enzyme reflux into the hepatic sinusoids due to a rapid increase in CBD pressure. This increases the permeability of hepatocytes and the release of enzymes into the bloodstream, resulting in damage due to bile-acid radicals, apoptosis, and the necrosis of hepatocytes [26]. The AST level better reflects the severity of cholangitis than the ALT level. The AST and ALT levels were higher in the stenting group than in the non-stenting group, indicating more severe inflammatory injury. In contrast, biliary stenting resulted in a dramatic improvement in the AST level after the removal of CBD stones under ERCP. Therefore, stent insertion accelerates recovery from acute cholangitis caused by CBD stones. In other words, transient biliary stenting can improve the clinical status of patients with high ALT and AST levels.

In clinical practice, endoscopists perform biliary stenting for patients at high risk of recurrence. Our results suggest the feasibility of preventive stenting in cases of lithotripsy or a difficult stone. Additionally, for patients with a high initial AST level, biliary stenting accelerated recovery from cholangitis. Therefore, a further confirmative prospective study is needed.

This study has several limitations. First, the patients had to undergo one or more unnecessary endoscopy procedures to remove the stent. Second, it was a single-center retrospective study; thus, there is a possibility of bias in the evaluation of outcomes. Third, endoscopic placement of an endoprosthesis reduced the risk of stone recurrence and improved liver function but did not shorten the hospital stay. This was probably due to the worse clinical status of patients in the stenting group. Fourth, residual stone and recurrent stone are theoretically different. However, residual stone and true recurrent stone are difficult to distinguish in the clinical setting and the two terms are sometimes confused or used interchangeably.

In conclusion, biliary stenting after the removal of CBD stones reduced the rate of stone recurrence and ameliorated severe cholangitis without causing severe complications. Preventive biliary stenting is a safe and effective treatment for CBD stones.

1. Biliary stenting after the removal of common bile duct (CBD) stones reduced the rate of stone recurrence and ameliorated severe cholangitis without causing severe complications.

2. In terms of safety and effectiveness, preventive biliary stenting is a safe and effective treatment for CBD stones.