Literature DB >> 3282541

Overexpression, purification and properties of alcohol dehydrogenase IV from Saccharomyces cerevisiae.

C Drewke1, M Ciriacy.   

Abstract

We have purified ADHIV, a novel alcohol dehydrogenase (ADH) isozyme in the yeast Saccharomyces cerevisiae, after increasing the normally low amount of ADHIV protein in laboratory strains. This was done by overexpression of the structural gene (ADH4) on a 2micro-based multicopy vector. Characterization of the purified enzyme revealed a dimeric structure as well as a different substrate specificity and pH profile as compared to other alcohol dehydrogenase isozymes. On the other hand, we could demonstrate that ADHIV is activated by zinc ions, like the other yeast alcohol dehydrogenase isozymes, and not by ferrous ions, like a structurally similar alcohol dehydrogenase from the bacterium Zymomonas mobilis.

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Year:  1988        PMID： 3282541     DOI： 10.1016/0167-4781(88)90072-3

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  28 in total

1.  Genome-wide characterization of the Zap1p zinc-responsive regulon in yeast.

Authors:  T J Lyons; A P Gasch; L A Gaither; D Botstein; P O Brown; D J Eide
Journal:  Proc Natl Acad Sci U S A       Date:  2000-07-05       Impact factor: 11.205

2.  Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes.

Authors:  K A Walter; G N Bennett; E T Papoutsakis
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

3.  Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae.

Authors:  M F van Iersel; M H Eppink; W J van Berkel; F M Rombouts; T Abee
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

4.  Repression of ADH1 and ADH3 during zinc deficiency by Zap1-induced intergenic RNA transcripts.

Authors:  Amanda J Bird; Mat Gordon; David J Eide; Dennis R Winge
Journal:  EMBO J       Date:  2006-11-30       Impact factor: 11.598

5.  Ethanol formation in adh0 mutants reveals the existence of a novel acetaldehyde-reducing activity in Saccharomyces cerevisiae.

Authors:  C Drewke; J Thielen; M Ciriacy
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

6.  Evolution of the alcohol dehydrogenase (ADH) genes in yeast: characterization of a fourth ADH in Kluyveromyces lactis.

Authors:  D H Shain; C Salvadore; C L Denis
Journal:  Mol Gen Genet       Date:  1992-04

7.  Effects of elemental sulfur on the metabolism of the deep-sea hyperthermophilic archaeon Thermococcus strain ES-1: characterization of a sulfur-regulated, non-heme iron alcohol dehydrogenase.

Authors:  K Ma; H Loessner; J Heider; M K Johnson; M W Adams
Journal:  J Bacteriol       Date:  1995-08       Impact factor: 3.490

8.  The cellular economy of the Saccharomyces cerevisiae zinc proteome.

Authors:  Yirong Wang; Erin Weisenhorn; Colin W MacDiarmid; Claudia Andreini; Michael Bucci; Janet Taggart; Lucia Banci; Jason Russell; Joshua J Coon; David J Eide
Journal:  Metallomics       Date:  2018-12-12       Impact factor: 4.526

9.  Similarity of Escherichia coli propanediol oxidoreductase (fucO product) and an unusual alcohol dehydrogenase from Zymomonas mobilis and Saccharomyces cerevisiae.

Authors:  T Conway; L O Ingram
Journal:  J Bacteriol       Date:  1989-07       Impact factor: 3.490

10.  An unusual oxygen-sensitive, iron- and zinc-containing alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus.

Authors:  K Ma; M W Adams
Journal:  J Bacteriol       Date:  1999-02       Impact factor: 3.490
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