Mauro de Mesquita Souza Saraiva1, Candice Maria Cardoso Gomes de Leon2, Núbia Michelle Vieira da Silva2, Tânia Freitas Raso3, Patricia Pereira Serafini4, Patricia Emilia Naves Givisiez2, Wondwossen Abebe Gebreyes5,6, Celso José Bruno de Oliveira2,6. 1. Laboratory of Ornithopathology, Department of Veterinary Pathology, Sao Paulo State University, Jaboticabal, Brazil. 2. Department of Animal Science, Federal University of Paraiba, Areia, Brazil. 3. Department of Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil. 4. National Center for Wild Bird Conservation and Research, Chico Mendes Institute for Biodiversity Conservation, Florianopolis, Brazil. 5. Department of Preventive Veterinary Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA. 6. Global One Health Initiative, The Ohio State University, Columbus, Ohio, USA.
Abstract
Aim: Genomic analysis of a methicillin-resistant Staphylococcus aureus (MRSA) strain cultured from a non-migratory seabird at Fernando de Noronha Archipelago (Brazilian oceanic islands) was carried out to investigate the potential origin of MRSA genetic determinants in an ecological setting with minimal or absent antimicrobial selective pressure, and minimal interaction with humans and domestic animals. Results: The study determined mecA gene homology and the phylogenetic relatedness with mecA described in Staphylococcus sciuri, which was the major Staphylococcus spp. cultured from the birds. Our findings corroborate in silico assumptions that the mecA gene in MRSA strains clinically relevant for humans and animals originates from S. sciuri ancestors. Conclusion: Coagulase-negative staphylococci seem to be natural reservoirs of methicillin-resistant genes to S. aureus, even in environments with very low antimicrobial selection pressure.
Aim: Genomic analysis of a methicillin-resistant Staphylococcus aureus (MRSA) strain cultured from a non-migratory seabird at Fernando de Noronha Archipelago (Brazilian oceanic islands) was carried out to investigate the potential origin of MRSA genetic determinants in an ecological setting with minimal or absent antimicrobial selective pressure, and minimal interaction with humans and domestic animals. Results: The study determined mecA gene homology and the phylogenetic relatedness with mecA described in Staphylococcus sciuri, which was the major Staphylococcus spp. cultured from the birds. Our findings corroborate in silico assumptions that the mecA gene in MRSA strains clinically relevant for humans and animals originates from S. sciuri ancestors. Conclusion: Coagulase-negative staphylococci seem to be natural reservoirs of methicillin-resistant genes to S. aureus, even in environments with very low antimicrobial selection pressure.
Authors: Vanessa Silva; Ana Filipa Lopes; Vanessa Soeiro; Manuela Caniça; Vera Manageiro; José Eduardo Pereira; Luís Maltez; José Luis Capelo; Gilberto Igrejas; Patrícia Poeta Journal: Antibiotics (Basel) Date: 2022-02-12