| Literature DB >> 32807944 |
Asmaa Hachim1, Niloufar Kavian2,3,4, Carolyn A Cohen1, Alex W H Chin5, Daniel K W Chu5, Chris K P Mok1, Owen T Y Tsang6, Yiu Cheong Yeung6, Ranawaka A P M Perera5, Leo L M Poon1,5, J S Malik Peiris1,5, Sophie A Valkenburg7.
Abstract
The SARS-CoV-2 virus emerged in December 2019 and has caused a worldwide pandemic due to the lack of any pre-existing immunity. Accurate serology testing is urgently needed to help diagnose infection, determine past exposure of populations and assess the response to a future vaccine. The landscape of antibody responses to SARS-CoV-2 is unknown. In this study, we utilized the luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19. We identified new targets of the immune response to SARS-CoV-2 and show that nucleocapsid, open reading frame (ORF)8 and ORF3b elicit the strongest specific antibody responses. ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity. Our findings could be used to develop second-generation diagnostic tests to improve serological assays for COVID-19 and are important in understanding pathogenicity.Entities:
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Year: 2020 PMID： 32807944 DOI： 10.1038/s41590-020-0773-7
Source DB: PubMed Journal: Nat Immunol ISSN： 1529-2908 Impact factor: 25.606