Xin Li1, Mariano Simón Pedano1, Shuchen Li2, Zheyi Sun2, Charlotte Jeanneau2, Imad About3, Esther Hauben4, Zhi Chen2, Kirsten Van Landuyt1, Bart Van Meerbeek5. 1. KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & UZ Leuven (University Hospitals Leuven), Dentistry, Leuven, Belgium. 2. Wuhan University, School and Hospital of Stomatology, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine of Ministry of Education, Wuhan, PR China. 3. Aix Marseille Univ, CNRS, ISM, Inst Movement Sci, Marseille, France. 4. Laboratory for Pathology, UZ Leuven & Department of Imaging and Pathology, Translational Cell and Tissue Research, KU, Leuven, Belgium. 5. KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & UZ Leuven (University Hospitals Leuven), Dentistry, Leuven, Belgium. Electronic address: bart.vanmeerbeek@kuleuven.be.
Abstract
OBJECTIVES: To investigate the pulpal repair potential of an experimental zirconium-oxide containing tricalcium-silicate cement, referred to as 'TCS 50'. MATERIALS AND METHODS: The effect of TCS 50 on viability, proliferation, migration, and odontoblastic differentiation of human dental pulp cells (HDPCs) was assessed using XTT assay, in-vitro wound healing assay and RT-PCR, respectively. Additionally, the pulp-capping potential was evaluated using a vital human tooth model. Statistical analysis was performed using non-parametric Kruskal-Wallis test and post-hoc test (Mann-Whitney U test). The tests were performed at a significance level of α = 0.05. RESULTS: The effect of TCS 50 towards HDPCs was dose dependent. Undiluted TCS 50 extract showed no immediate adverse impact on cell viability (p > .05); however, it significantly inhibited proliferation and migration of HDPCs (p < .05). A 25% diluted TCS 50 extract showed no significant effect on cell viability, proliferation or migration (p > .05), and it significantly enhanced odontoblastic differentiation of HDPCs (p < .05). In pulps capped with TCS 50 for both 2 and 4 weeks, H&E staining revealed a normal morphology of pulp tissue; mineralized foci with cellular components entrapped in the matrix were formed underneath the exposure site. Collagen I expression was weak within the matrix of mineralized foci, while the expression of nestin was positive for entrapped cellular components within the mineralized foci, indicating that the formed mineralized foci corresponded to an initial form of reparative dentin formation. CONCLUSION: TCS 50 is capable of generating an early pulp-healing reaction and therefore could serve as a promising pulp-capping agent.
OBJECTIVES: To investigate the pulpal repair potential of an experimental zirconium-oxide containing tricalcium-silicate cement, referred to as 'TCS 50'. MATERIALS AND METHODS: The effect of TCS 50 on viability, proliferation, migration, and odontoblastic differentiation of human dental pulp cells (HDPCs) was assessed using XTT assay, in-vitro wound healing assay and RT-PCR, respectively. Additionally, the pulp-capping potential was evaluated using a vital human tooth model. Statistical analysis was performed using non-parametric Kruskal-Wallis test and post-hoc test (Mann-Whitney U test). The tests were performed at a significance level of α = 0.05. RESULTS: The effect of TCS 50 towards HDPCs was dose dependent. Undiluted TCS 50 extract showed no immediate adverse impact on cell viability (p > .05); however, it significantly inhibited proliferation and migration of HDPCs (p < .05). A 25% diluted TCS 50 extract showed no significant effect on cell viability, proliferation or migration (p > .05), and it significantly enhanced odontoblastic differentiation of HDPCs (p < .05). In pulps capped with TCS 50 for both 2 and 4 weeks, H&E staining revealed a normal morphology of pulp tissue; mineralized foci with cellular components entrapped in the matrix were formed underneath the exposure site. Collagen I expression was weak within the matrix of mineralized foci, while the expression of nestin was positive for entrapped cellular components within the mineralized foci, indicating that the formed mineralized foci corresponded to an initial form of reparative dentin formation. CONCLUSION:TCS 50 is capable of generating an early pulp-healing reaction and therefore could serve as a promising pulp-capping agent.
Authors: Ana Lozano-Guillén; Sergio López-García; Francisco Javier Rodríguez-Lozano; José Luis Sanz; Adrián Lozano; Carmen Llena; Leopoldo Forner Journal: Clin Oral Investig Date: 2022-09-02 Impact factor: 3.606
Authors: F J Rodríguez-Lozano; A Lozano; S López-García; D García-Bernal; J L Sanz; J Guerrero-Gironés; C Llena; L Forner; M Melo Journal: Clin Oral Investig Date: 2021-08-12 Impact factor: 3.573