Caroline M Ganobis1, M Sameer Al-Abdul-Wahid2, Simone Renwick1, Sandi Yen1,3, Charley Carriero1, Marc G Aucoin4, Emma Allen-Vercoe1. 1. Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada. 2. Advanced Analysis Centre, University of Guelph, Guelph, Ontario, Canada. 3. Oxford Centre of Microbiome Studies, Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, United Kingdom. 4. Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada.
Abstract
Metabolomic studies allow a deeper understanding of the processes of a given ecological community than nucleic acid-based surveys alone. In the case of the gut microbiota, a metabolic profile of, for example, a fecal sample provides details about the function and interactions within the distal region of the gastrointestinal tract, and such a profile can be generated in a number of different ways. This unit elaborates on the use of 1D 1 H NMR spectroscopy as a commonly used method to characterize small-molecule metabolites of the fecal metabonome (meta-metabolome). We describe a set of protocols for the preparation of fecal water extraction, storage, scanning, measurement of pH, and spectral processing and analysis. We also compare the effects of various sample storage conditions for processed and unprocessed samples to provide a framework for comprehensive analysis of small molecules from stool-derived samples.
Metabolomic studies allow a deeper understanding of the processes of a given ecological community than nucleic acid-based surveys alone. In the case of the gut microbiota, a metabolic profile of, for example, a fecal sample provides details about the function and interactions within the distal region of the gastrointestinal tract, and such a profile can be generated in a number of different ways. This unit elaborates on the use of 1D 1 H NMR spectroscopy as a commonly used method to characterize small-molecule metabolites of the fecal metabonome (meta-metabolome). We describe a set of protocols for the preparation of fecal water extraction, storage, scanning, measurement of pH, and spectral processing and analysis. We also compare the effects of various sample storage conditions for processed and unprocessed samples to provide a framework for comprehensive analysis of small molecules from stool-derived samples.
Authors: Julie A K McDonald; Kathleen Schroeter; Susana Fuentes; Ineke Heikamp-Dejong; Cezar M Khursigara; Willem M de Vos; Emma Allen-Vercoe Journal: J Microbiol Methods Date: 2013-08-27 Impact factor: 2.363
Authors: Doris M Jacobs; Nancy Deltimple; Ewoud van Velzen; Ferdi A van Dorsten; Max Bingham; Elaine E Vaughan; John van Duynhoven Journal: NMR Biomed Date: 2008-07 Impact factor: 4.044
Authors: Santosh Lamichhane; Christian C Yde; Mette S Schmedes; Henrik Max Jensen; Sebastian Meier; Hanne Christine Bertram Journal: Anal Chem Date: 2015-05-28 Impact factor: 6.986
Authors: Siamak Ravanbakhsh; Philip Liu; Trent C Bjorndahl; Trent C Bjordahl; Rupasri Mandal; Jason R Grant; Michael Wilson; Roman Eisner; Igor Sinelnikov; Xiaoyu Hu; Claudio Luchinat; Russell Greiner; David S Wishart Journal: PLoS One Date: 2015-05-27 Impact factor: 3.240
Authors: Tiffany L Weir; Daniel K Manter; Amy M Sheflin; Brittany A Barnett; Adam L Heuberger; Elizabeth P Ryan Journal: PLoS One Date: 2013-08-06 Impact factor: 3.240
Authors: Jennifer L MacNicol; Simone Renwick; Caroline M Ganobis; Emma Allen-Vercoe; Jeffery Scott Weese; Wendy Pearson Journal: Animals (Basel) Date: 2022-08-08 Impact factor: 3.231