| Literature DB >> 32778833 |
Venkata P Dandey1, William C Budell1, Hui Wei1, Daija Bobe1, Kashyap Maruthi1, Mykhailo Kopylov1, Edward T Eng1, Peter A Kahn2, Jenny E Hinshaw3, Nidhi Kundu3, Crina M Nimigean4, Chen Fan4, Nattakan Sukomon4, Seth A Darst5, Ruth M Saecker5, James Chen5, Brandon Malone5, Clinton S Potter1,6, Bridget Carragher7,8.
Abstract
We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto the surface of a nanowire EM grid, and the mixing reaction stops when the grid is vitrified in liquid ethane ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.Entities:
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Year: 2020 PMID： 32778833 PMCID： PMC7799389 DOI： 10.1038/s41592-020-0925-6
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547