Literature DB >> 3277161

Mechanism of autonomous control of the Escherichia coli F plasmid: purification and characterization of the repE gene product.

L Masson1, D S Ray.   

Abstract

E protein, the 29 Kd repE gene product, is essential for the replication of the Escherichia coli F plasmid. The repE gene has been cloned and expressed in an inducible ATG-fusion vector, and the protein product has been purified to homogeneity. The purified E protein is present as a dimer in solution and binds specifically to both the 19-bp direct repeats (incB) found within the mini-F origin of replication ori2 and the repE operator DNA. Examination of the amino acid sequence of E protein revealed a consensus sequence for DNA binding.

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Year:  1988        PMID： 3277161      PMCID： PMC334669          DOI： 10.1093/nar/16.2.413

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  RepC is rate limiting for pT181 plasmid replication.

Authors:  J N Manch-Citron; M L Gennaro; S Majumder; R P Novick
Journal:  Plasmid       Date:  1986-09       Impact factor: 3.466

3.  Lac repressor-operator interaction. I. Equilibrium studies.

Authors:  A D Riggs; H Suzuki; S Bourgeois
Journal:  J Mol Biol       Date:  1970-02-28       Impact factor: 5.469

4.  Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.

Authors:  M Fried; D M Crothers
Journal:  Nucleic Acids Res       Date:  1981-12-11       Impact factor: 16.971

5.  Genetic evidence that control of F replication is negative.

Authors:  B C Kline; R W Seelke
Journal:  Mol Gen Genet       Date:  1982

6.  Role of nine repeating sequences of the mini-F genome for expression of F-specific incompatibility phenotype and copy number control.

Authors:  H Tsutsui; A Fujiyama; T Murotsu; K Matsubara
Journal:  J Bacteriol       Date:  1983-07       Impact factor: 3.490

7.  Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments.

Authors:  N Komai; T Nishizawa; Y Hayakawa; T Murotsu; K Matsubara
Journal:  Mol Gen Genet       Date:  1982

8.  Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid.

Authors:  T Murotsu; K Matsubara; H Sugisaki; M Takanami
Journal:  Gene       Date:  1981-11       Impact factor: 3.688

9.  An Mr 29000 protein is essential for mini-F maintenance in E. coli.

Authors:  L A Watson; S H Phua; P L Bergquist; H E Lane
Journal:  Gene       Date:  1982-09       Impact factor: 3.688

10.  Separation of the minimal replication region of the F plasmid into a replication origin segment and a trans-acting segment.

Authors:  A Tolun; D R Helinski
Journal:  Mol Gen Genet       Date:  1982
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  8 in total

1.  Predicting plasmid promiscuity based on genomic signature.

Authors:  Haruo Suzuki; Hirokazu Yano; Celeste J Brown; Eva M Top
Journal:  J Bacteriol       Date:  2010-09-17       Impact factor: 3.490

2.  Replication of the broad-host-range plasmid RK2: direct measurement of intracellular concentrations of the essential TrfA replication proteins and their effect on plasmid copy number.

Authors:  R H Durland; D R Helinski
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

3.  Characterization of the basic replicon of pCM1, a narrow-host-range plasmid from the moderate halophile Chromohalobacter marismortui.

Authors:  E Mellado; J A Asturias; J J Nieto; K N Timmis; A Ventosa
Journal:  J Bacteriol       Date:  1995-06       Impact factor: 3.490

4.  Specific binding of the replication protein of plasmid pPS10 to direct and inverted repeats is mediated by an HTH motif.

Authors:  D García de Viedma; A Serrano-López; R Díaz-Orejas
Journal:  Nucleic Acids Res       Date:  1995-12-25       Impact factor: 16.971

5.  Expression and purification of F-plasmid RepE and preliminary X-ray crystallographic study of its complex with operator DNA.

Authors:  Akira Nakamura; Chieko Wada; Kunio Miki
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-03-30

6.  Site-specific proteolysis of mini-F plasmid replication protein RepE destroys initiator function and generates an incompatibility substance.

Authors:  B C Kline; G S Sandhu; B W Eckloff; R A Aleff
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

7.  Replication initiator protein RepE of mini-F plasmid: functional differentiation between monomers (initiator) and dimers (autogenous repressor).

Authors:  M Ishiai; C Wada; Y Kawasaki; T Yura
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-26       Impact factor: 11.205

8.  DNA-binding domain of the RepE initiator protein of mini-F plasmid: involvement of the carboxyl-terminal region.

Authors:  F Matsunaga; Y Kawasaki; M Ishiai; K Nishikawa; T Yura; C Wada
Journal:  J Bacteriol       Date:  1995-04       Impact factor: 3.490
  8 in total

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