| Literature DB >> 32770507 |
Laureano D Asico1, Selim Rozyyev2,3, Annabelle M Crusan4, Pedro A Jose2, Van Anthony M Villar5.
Abstract
Numerous G protein-coupled receptors (GPCRs) and GPCR-signaling molecules reside in lipid rafts and thus, are inherently regulated in these microdomains. However, the limitations of current methods to investigate lipid raft biology and GPCR activity in situ have hindered the complete understanding of the molecular underpinnings of GPCR trafficking and signaling, especially in the whole organism. This book chapter details an innovative in vivo approach to study the crucial role of lipid rafts on the workings of GPCRs in the mouse kidney. This protocol involves the use of a modified mini osmotic pump to deliver an agent that selectively disrupts the lipid raft in the kidney.Entities:
Keywords: Kidney; Lipid raft; Methyl-β-cyclodextrin; Mini osmotic pump; Mouse; α-Cyclodextrin
Year: 2021 PMID： 32770507 PMCID： PMC7597372 DOI： 10.1007/978-1-0716-0814-2_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745