Literature DB >> 3276795

An inhibition enzyme immunoassay for estimating relative antibody affinity and affinity heterogeneity.

S Rath1, C M Stanley, M W Steward.   

Abstract

A method to measure the relative affinity of antibodies using an inhibition enzyme immunoassay is described. It is validated using monoclonal antibodies of defined affinity characteristics and by comparison with conventional methods of affinity measurement. The method allows measurement of the relative affinity of low levels of antibody, and the calculation of an empirical estimate of the heterogeneity of affinity in antibody populations.

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Year:  1988        PMID： 3276795     DOI： 10.1016/0022-1759(88)90204-9

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  39 in total

1.  In vitro selection and affinity maturation of antibodies from a naive combinatorial immunoglobulin library.

Authors:  H Gram; L A Marconi; C F Barbas; T A Collet; R A Lerner; A S Kang
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205

2.  Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem.

Authors:  C F Barbas; J D Bain; D M Hoekstra; R A Lerner
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-15       Impact factor: 11.205

3.  Identification of an immunodominant neutralizing and protective epitope from measles virus fusion protein by using human sera from acute infection.

Authors:  S F Atabani; O E Obeid; D Chargelegue; P Aaby; H Whittle; M W Steward
Journal:  J Virol       Date:  1997-10       Impact factor: 5.103

4.  Antibodies of different human IgG subclasses show distinct patterns of affinity maturation after immunization with keyhole limpet haemocyanin.

Authors:  M E Devey; K M Bleasdale-Barr; P Bird; P L Amlot
Journal:  Immunology       Date:  1990-06       Impact factor: 7.397

5.  Diagnostic evaluation of a nanobody with picomolar affinity toward the protease RgpB from Porphyromonas gingivalis.

Authors:  Peter Durand Skottrup; Paul Leonard; Jakub Zbigniew Kaczmarek; Florian Veillard; Jan Johannes Enghild; Richard O'Kennedy; Aneta Sroka; Rasmus Prætorius Clausen; Jan Potempa; Erik Riise
Journal:  Anal Biochem       Date:  2011-04-20       Impact factor: 3.365

6.  Relative avidities of human immunoglobulin G antibodies for streptococcal pyrogenic exotoxins A and B.

Authors:  E M Mascini; M Jansze; J Verhoef; H van Dijk
Journal:  Clin Diagn Lab Immunol       Date:  1999-11

7.  A parallel panning scheme used for selection of a GluA4-specific Fab targeting the ligand-binding domain.

Authors:  Rasmus P Clausen; Andreas Ø Mohr; Erik Riise; Anders A Jensen; Avinash Gill; Dean R Madden; Jette S Kastrup; Peter D Skottrup
Journal:  Int J Biol Macromol       Date:  2016-07-08       Impact factor: 6.953

8.  Efficient method for constructing comprehensive murine Fab antibody libraries displayed on phage.

Authors:  H Orum; P S Andersen; A Oster; L K Johansen; E Riise; M Bjørnvad; I Svendsen; J Engberg
Journal:  Nucleic Acids Res       Date:  1993-09-25       Impact factor: 16.971

9.  Recombinant single-chain variable fragment antibodies directed against Clostridium difficile toxin B produced by use of an optimized phage display system.

Authors:  Xiao K Deng; Lance A Nesbit; K John Morrow
Journal:  Clin Diagn Lab Immunol       Date:  2003-07

10.  Combinatorial autoantibodies to dihydrolipoamide acetyltransferase, the major autoantigen of primary biliary cirrhosis.

Authors:  S Cha; P S Leung; M E Gershwin; M P Fletcher; A A Ansari; R L Coppel
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-15       Impact factor: 11.205
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