Riley J Giesler1, Patrick W Erickson1, Michael S Kay2. 1. Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Room 4100, Salt Lake City, UT 84112-5650, United States. 2. Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Room 4100, Salt Lake City, UT 84112-5650, United States. Electronic address: kay@biochem.utah.edu.
Abstract
Native chemical ligation has enabled the chemical synthesis of proteins for a wide variety of applications (e.g., mirror-image proteins). However, inefficiencies of this chemoselective ligation in the context of large or otherwise challenging protein targets can limit the practical scope of chemical protein synthesis. In this review, we focus on recent developments aimed at enhancing and expanding native chemical ligation for challenging protein syntheses. Chemical auxiliaries, use of selenium chemistry, and templating all enable ligations at otherwise suboptimal junctions. The continuing development of these tools is making the chemical synthesis of large proteins increasingly accessible.
Native chemical ligation has enabled the chemical synthesis of proteins for a wide variety of applications (e.g., mirror-image n class="Chemical">proteins). However, inefficiencies of this chemoselective ligation in the context of large or otherwise challenging protein targets can limit the practical scope of chemical protein synthesis. In this review, we focus on recent developments aimed at enhancing and expanding native chemical ligation for challenging protein syntheses. Chemical auxiliaries, use of selenium chemistry, and templating all enable ligations at otherwise suboptimal junctions. The continuing development of these tools is making the chemical synthesis of large proteins increasingly accessible.
Authors: James M Fulcher; Mark E Petersen; Riley J Giesler; Zachary S Cruz; Debra M Eckert; J Nicholas Francis; Eric M Kawamoto; Michael T Jacobsen; Michael S Kay Journal: Org Biomol Chem Date: 2019-12-03 Impact factor: 3.876