Literature DB >> 32744428

An Acid-Activatable Fluorescence Probe for Imaging Osteocytic Bone Resorption Activity in Deep Bone Cavities.

Ryu Hashimoto1, Masafumi Minoshima1, Junichi Kikuta2,3, Shinya Yari2, Steven D Bull4, Masaru Ishii2,3, Kazuya Kikuchi1,3,5.   

Abstract

A rationally designed pH-activatable fluorescent probe (pHocas-RIS) has been used to measure localised pH levels in osteocytic lacunae in bone tissue. Conjugation of the moderate bone-binding drug risedronate to a pH-activatable BODIPY fluorophore enables the probe to penetrate osteocytic lacunae cavities that are embedded deep within the bone matrix. After injection of pHocas-RIS, any osteocytic lacunae caused by bone-resorbing osteocytes cause the probe to fluoresce in vivo, thus allowing imaging by intravital two-photon excitation microscopy. This pH responsive probe enabled the visualization of the bone mineralizing activities of acid producing osteocytes in real time, thus allowing the study of their central role in remodeling the bone-matrix in healthy and disease states.
© 2020 The Authors. Published by Wiley-VCH GmbH.

Entities:  

Keywords:  fluorescent probes; intravital imaging; osteocytes; osteocytic lacunae; pH-activatable probes

Mesh：

Substances：

Year:  2020        PMID： 32744428      PMCID： PMC7692916          DOI： 10.1002/anie.202006388

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  20 in total

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Authors:  R G Russell; M J Rogers
Journal:  Bone       Date:  1999-07       Impact factor: 4.398

Review 2.  Osteocyte: the unrecognized side of bone tissue.

Authors:  G Y Rochefort; S Pallu; C L Benhamou
Journal:  Osteoporos Int       Date:  2010-03-04       Impact factor: 4.507

3.  Dynamic visualization of RANKL and Th17-mediated osteoclast function.

Authors:  Junichi Kikuta; Yoh Wada; Toshiyuki Kowada; Ze Wang; Ge-Hong Sun-Wada; Issei Nishiyama; Shin Mizukami; Nobuhiko Maiya; Hisataka Yasuda; Atsushi Kumanogoh; Kazuya Kikuchi; Ronald N Germain; Masaru Ishii
Journal:  J Clin Invest       Date:  2013-01-16       Impact factor: 14.808

Review 4.  A review of osteocyte function and the emerging importance of sclerostin.

Authors:  Jocelyn T Compton; Francis Y Lee
Journal:  J Bone Joint Surg Am       Date:  2014-10-01       Impact factor: 5.284

5.  Real-time intravital imaging of pH variation associated with osteoclast activity.

Authors:  Hiroki Maeda; Toshiyuki Kowada; Junichi Kikuta; Masayuki Furuya; Mai Shirazaki; Shin Mizukami; Masaru Ishii; Kazuya Kikuchi
Journal:  Nat Chem Biol       Date:  2016-06-06       Impact factor: 15.040

6.  Intravital bone imaging by two-photon excitation microscopy to identify osteocytic osteolysis in vivo.

Authors:  Hiroshige Sano; Junichi Kikuta; Masayuki Furuya; Naoki Kondo; Naoto Endo; Masaru Ishii
Journal:  Bone       Date:  2015-01-24       Impact factor: 4.398

7.  Fluorescent Bisphosphonate and Carboxyphosphonate Probes: A Versatile Imaging Toolkit for Applications in Bone Biology and Biomedicine.

Authors:  Shuting Sun; Katarzyna M Błażewska; Anastasia P Kadina; Boris A Kashemirov; Xuchen Duan; James T Triffitt; James E Dunford; R Graham G Russell; Frank H Ebetino; Anke J Roelofs; Fraser P Coxon; Mark W Lundy; Charles E McKenna
Journal:  Bioconjug Chem       Date:  2015-12-08       Impact factor: 4.774

8.  Selective molecular imaging of viable cancer cells with pH-activatable fluorescence probes.

Authors:  Yasuteru Urano; Daisuke Asanuma; Yukihiro Hama; Yoshinori Koyama; Tristan Barrett; Mako Kamiya; Tetsuo Nagano; Toshiaki Watanabe; Akira Hasegawa; Peter L Choyke; Hisataka Kobayashi
Journal:  Nat Med       Date:  2008-12-07       Impact factor: 53.440

Review 9.  Physiological and pathological osteocytic osteolysis.

Authors:  Elena Tsourdi; Katharina Jähn; Martina Rauner; Björn Busse; Lynda F Bonewald
Journal:  J Musculoskelet Neuronal Interact       Date:  2018-09-01       Impact factor: 2.041

Review 10.  Modalities for Visualization of Cortical Bone Remodeling: The Past, Present, and Future.

Authors:  Kimberly D Harrison; David M L Cooper
Journal:  Front Endocrinol (Lausanne)       Date:  2015-08-11       Impact factor: 5.555
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  3 in total

Review 1.  Activatable NIR-II organic fluorescent probes for bioimaging.

Authors:  Xiaoning Zhang; Shasha Li; Huizhen Ma; Hao Wang; Ruiping Zhang; Xiao-Dong Zhang
Journal:  Theranostics       Date:  2022-04-11       Impact factor: 11.600

2.  An Acid-Activatable Fluorescence Probe for Imaging Osteocytic Bone Resorption Activity in Deep Bone Cavities.

Authors:  Ryu Hashimoto; Masafumi Minoshima; Junichi Kikuta; Shinya Yari; Steven D Bull; Masaru Ishii; Kazuya Kikuchi
Journal:  Angew Chem Int Ed Engl       Date:  2020-09-08       Impact factor: 15.336

3.  A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy-Induced Cancer Cell Death.

Authors:  Nicole D Barth; Lorena Mendive-Tapia; Ramon Subiros-Funosas; Ouldouz Ghashghaei; Rodolfo Lavilla; Laura Maiorino; Xue-Yan He; Ian Dransfield; Mikala Egeblad; Marc Vendrell
Journal:  Angew Chem Int Ed Engl       Date:  2021-12-16       Impact factor: 16.823
  3 in total

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