| Literature DB >> 3273856 |
F S Hagen1, C L Gray, J L Kuijper.
Abstract
Once a cDNA library has been constructed, it is very useful to be able to easily assess the quality of the library. Because actin is a ubiquitous sequence, this assessment has been accomplished by probing filter lifts and Southern blots of libraries with an actin cDNA probe. These methods provide information about the percent of actin-positive clones and the degree of completeness of cDNA clones in a library. Results of these methods are correlated with the success of finding full-length clones of interest.Mesh：
Substances：
Year: 1988 PMID： 3273856
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993