Literature DB >> 1768589

Molecular approaches for analyzing differential gene expression: differential cDNA library construction and screening.

J P Calvet1.   

Abstract

Complementary deoxyribonucleic acid (cDNA) libraries can be used as a means to isolate and identify cell-specific messenger ribonucleic acid (mRNA) sequences. The basic elements of cDNA library construction and screening are reviewed in the context of analyzing differentially expressed mRNAs. A brief overview of the recombinant DNA systems applied to cDNA library construction and the principles of screening cDNA libraries by plaque hybridization are provided. Methods for comparing mRNA populations by differential screening and by competition hybridization are discussed, and methods for constructing subtracted cDNA libraries, enriched in differentially expressed sequences, are presented. Also reviewed are the analysis of differentially expressed cDNAs by Southern and Northern hybridization, RNase protection, polymerase chain reaction, and sequencing.

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Year:  1991        PMID： 1768589     DOI： 10.1007/bf00857891

Source DB:  PubMed          Journal:  Pediatr Nephrol        ISSN： 0931-041X            Impact factor:   3.714


  41 in total

1.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

2.  Rapid amplification of lambda gt11 bacteriophage library inserts from plaques using the polymerase chain reaction (PCR).

Authors:  D M Dorfman; L I Zon; S H Orkin
Journal:  Biotechniques       Date:  1989-06       Impact factor: 1.993

3.  Cloning of a cDNA for a T cell-specific serine protease from a cytotoxic T lymphocyte.

Authors:  H K Gershenfeld; I L Weissman
Journal:  Science       Date:  1986-05-16       Impact factor: 47.728

4.  Isolation of differentially expressed genes.

Authors:  T D Sargent
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

5.  Isolation of cDNA clones encoding T cell-specific membrane-associated proteins.

Authors:  S M Hedrick; D I Cohen; E A Nielsen; M M Davis
Journal:  Nature       Date:  1984 Mar 8-14       Impact factor: 49.962

6.  High-efficiency cloning of full-length cDNA.

Authors:  H Okayama; P Berg
Journal:  Mol Cell Biol       Date:  1982-02       Impact factor: 4.272

7.  Two clusters of genes for major chorion proteins of Drosophila melanogaster.

Authors:  A C Spradling; M E Digan; A P Mahowald; M Scott; E A Craig
Journal:  Cell       Date:  1980-04       Impact factor: 41.582

8.  The chorion genes of the medfly, Ceratitis capitata. II. Characterization of three novel cDNA clones obtained by differential screening of an ovarian library.

Authors:  P P Tolias; M Konsolaki; K Komitopoulou; F C Kafatos
Journal:  Dev Biol       Date:  1990-07       Impact factor: 3.582

9.  Y-encoded, species-specific DNA in mice: evidence that the Y chromosome exists in two polymorphic forms in inbred strains.

Authors:  E E Lamar; E Palmer
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

10.  Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.

Authors:  J R Duguid; R G Rohwer; B Seed
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205
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  1 in total

Review 1.  Human cell lines as models for multidrug resistance in solid tumours.

Authors:  M Clynes; M Heenan; K Hall
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058
  1 in total

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