Natalia Ulloa1, Marcelo Villagrán2, Benilde Riffo3, Andrea Gleisner4, Fanny Petermann-Rocha5, Lorena Mardones2, Ana María Leiva6, María Adela Martínez-Sanguinetti7, Carlos Celis-Morales8. 1. Universidad de Concepción, Concepción, Chile. 2. Departamento de ciencias Básicas, Facultad de Medicina, Universidad Católica de la Santísima Concepción, Concepción, Chile. 3. Departamento de Bioquímica clínica e Inmunología, Facultad de Farmacia, Universidad de Concepción, Concepción, Chile. 4. Departamento de Pediatría, Facultad de Medicina, Universidad de Concepción, Concepción, Chile. 5. Institute of Health and Wellbeing, University of Glasgow, Glasgow, United Kingdom. 6. Instituto de Anatomía, Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile. 7. Instituto de Farmacia, Facultad de ciencias, Universidad Austral de Chile, Valdivia, Chile. 8. BHF Glasgow cardiovascular Research centre, Institute of cardiovascular and Medical Sciences, University of Glasgow, Glasgow, United Kingdom.
Abstract
INTRODUCTION: Obesity is considered a chronic inflammatory disease with an important genetic component. Although several studies have reported an association between the FTO (fat-mass associated gene) and adiposity in children, there is limited evidence in the Chilean population. OBJECTIVE: To deter mine the association between the polymorphism rs9939609 of the FTO gene and markers of adipo sity in Chilean children. PATIENTS AND METHOD: Cross-sectional study which included 361 children aged between 6 and 11 years (50% were girls). Between March and June 2008, clinical data and blood sample collection was carried out. The rs9939609 single-nucleotide polymorphism (SNP) of the FTO gene, was determined using the genomic DNA extracted from leukocytes, using the QIAamp DNA Blood Mini Kit (Qiagen GmbH, Hilden, Germany).The adiposity markers included were body mass index (BMI), waist circumference (WC), body fat, and WC/H index; which were later compared adjusted by sex, age, and Tanner stage. Linear regression analyses were conducted to detect the association between the polymorphism and obesity markers. RESULTS: After adjusting the models by age, sex, and Tanner stage, we found a significant association between the polymorphism and markers of adiposity. For each extra copy of the risk allele, we found an increase of 2.47 kg body weight (95% CI: 1.39-3.55); 1.06 kg/m2 BMI (95% CI: 0.56-1.54); 2.55 cm WC, (95% CI: 1.26-3.85); and 1.98% body fat (95% CI: 0.78-3.19). When converting adiposity markers to z-score, we found that WC/height index shows the strongest association with the risk allele FTO. CONCLUSION: This study supports the association between the rs9939609 SNP of the FTO gene and overall and central adiposity markers in Chilean children.
INTRODUCTION:Obesity is considered a chronic inflammatory disease with an important genetic component. Although several studies have reported an association between the FTO (fat-mass associated gene) and adiposity in children, there is limited evidence in the Chilean population. OBJECTIVE: To deter mine the association between the polymorphism rs9939609 of the FTO gene and markers of adipo sity in Chilean children. PATIENTS AND METHOD: Cross-sectional study which included 361 children aged between 6 and 11 years (50% were girls). Between March and June 2008, clinical data and blood sample collection was carried out. The rs9939609 single-nucleotide polymorphism (SNP) of the FTO gene, was determined using the genomic DNA extracted from leukocytes, using the QIAamp DNA Blood Mini Kit (Qiagen GmbH, Hilden, Germany).The adiposity markers included were body mass index (BMI), waist circumference (WC), body fat, and WC/H index; which were later compared adjusted by sex, age, and Tanner stage. Linear regression analyses were conducted to detect the association between the polymorphism and obesity markers. RESULTS: After adjusting the models by age, sex, and Tanner stage, we found a significant association between the polymorphism and markers of adiposity. For each extra copy of the risk allele, we found an increase of 2.47 kg body weight (95% CI: 1.39-3.55); 1.06 kg/m2 BMI (95% CI: 0.56-1.54); 2.55 cm WC, (95% CI: 1.26-3.85); and 1.98% body fat (95% CI: 0.78-3.19). When converting adiposity markers to z-score, we found that WC/height index shows the strongest association with the risk allele FTO. CONCLUSION: This study supports the association between the rs9939609 SNP of the FTO gene and overall and central adiposity markers in Chilean children.
Authors: Ana Paula Sehn; Caroline Brand; João Francisco de Castro Silveira; Lars Bo Andersen; Anelise Reis Gaya; Pâmela Ferreira Todendi; Andréia Rosane de Moura Valim; Cézane Priscila Reuter Journal: BMC Cardiovasc Disord Date: 2022-03-09 Impact factor: 2.298