Michelle C Sabo1, Dara A Lehman2,3, Jillian C Pintye2, Bingjie Wang3, Alison L Drake2, John Kinuthia2,4, Lusi Osborn5, Daniel Matemo5, Barbra A Richardson2,6,7, Julie Overbaugh7, Grace John-Stewart1,2,8,9, Susan M Graham1,2,8. 1. Department of Medicine. 2. Department of Global Health. 3. Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA. 4. Department of Research and Programs, Kenyatta National Hospital. 5. Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya. 6. Department of Biostatistics. 7. Vaccine and Infectious Disease Division. 8. Department of Epidemiology, University of Washington. 9. Department of Pediatrics, Seattle Children's Hospital, Seattle, Washington, USA.
Abstract
OBJECTIVE: To evaluate the relationship between cervical cytokine/chemokine concentrations and HIV-1 acquisition in peripartum Kenyan women. DESIGN: Nested case-control study. METHODS: Women participating in a prospective study of peripartum HIV acquisition in Kenya (the Mama Salama Study), were tested for HIV-1 at 1-3 month intervals during pregnancy and through 9 months postpartum. Cases positive for HIV-1 RNA during follow-up (N = 14), were matched 3 : 1 with HIV-negative controls (N = 42) based on age, marital status, partner HIV-1 status, transactional sex, and timing of cervical swab collection. Concentrations of five cytokines (IL-1β, IL-6, IL-10, IFNγ, and TNFα) and four chemokines (IL-8, C-X-C motif chemokine ligand 10 (CXCL10), macrophage inflammatory protein-1 α, and macrophage inflammatory protein-1 β) were measured from cervical swabs collected at the visit prior to HIV-1 diagnosis (cases) or matched gestational/postpartum time (controls). Cytokine/chemokine concentrations were compared between cases and controls using Wilcoxon rank-sum tests. Principal component analysis was used to create a summary score for closely correlated cytokines/chemokines. Associations with HIV-1 acquisition were analyzed using conditional logistic regression. Path analysis was used to evaluate hypothesized relationships between CXCL10, vaginal washing, Nugent score, and HIV-1 acquisition. RESULTS: Conditional logistic regression analysis demonstrated an association between increased concentrations of CXCL10 and HIV-1 acquisition (odds ratio = 1.74, 95% confidence interval 1.04, 2.93; P = 0.034). Path analysis confirmed a positive independent association between higher concentrations of CXCL10 and HIV-1 acquisition (path coefficient = 0.37, 95% confidence interval 0.15, 0.59; P < 0.001). CONCLUSION: HIV-1 acquisition was associated with increased cervical concentrations of CXCL10 in pregnant and postpartum women.
OBJECTIVE: To evaluate the relationship between cervical cytokine/chemokine concentrations and HIV-1 acquisition in peripartum Kenyan women. DESIGN: Nested case-control study. METHODS: Women participating in a prospective study of peripartum HIV acquisition in Kenya (the Mama Salama Study), were tested for HIV-1 at 1-3 month intervals during pregnancy and through 9 months postpartum. Cases positive for HIV-1 RNA during follow-up (N = 14), were matched 3 : 1 with HIV-negative controls (N = 42) based on age, marital status, partner HIV-1 status, transactional sex, and timing of cervical swab collection. Concentrations of five cytokines (IL-1β, IL-6, IL-10, IFNγ, and TNFα) and four chemokines (IL-8, C-X-C motif chemokine ligand 10 (CXCL10), macrophage inflammatory protein-1 α, and macrophage inflammatory protein-1 β) were measured from cervical swabs collected at the visit prior to HIV-1 diagnosis (cases) or matched gestational/postpartum time (controls). Cytokine/chemokine concentrations were compared between cases and controls using Wilcoxon rank-sum tests. Principal component analysis was used to create a summary score for closely correlated cytokines/chemokines. Associations with HIV-1 acquisition were analyzed using conditional logistic regression. Path analysis was used to evaluate hypothesized relationships between CXCL10, vaginal washing, Nugent score, and HIV-1 acquisition. RESULTS: Conditional logistic regression analysis demonstrated an association between increased concentrations of CXCL10 and HIV-1 acquisition (odds ratio = 1.74, 95% confidence interval 1.04, 2.93; P = 0.034). Path analysis confirmed a positive independent association between higher concentrations of CXCL10 and HIV-1 acquisition (path coefficient = 0.37, 95% confidence interval 0.15, 0.59; P < 0.001). CONCLUSION: HIV-1 acquisition was associated with increased cervical concentrations of CXCL10 in pregnant and postpartum women.
Authors: Lindi Masson; Jo-Ann S Passmore; Lenine J Liebenberg; Lise Werner; Cheryl Baxter; Kelly B Arnold; Carolyn Williamson; Francesca Little; Leila E Mansoor; Vivek Naranbhai; Douglas A Lauffenburger; Katharina Ronacher; Gerhard Walzl; Nigel J Garrett; Brent L Williams; Mara Couto-Rodriguez; Mady Hornig; W Ian Lipkin; Anneke Grobler; Quarraisha Abdool Karim; Salim S Abdool Karim Journal: Clin Infect Dis Date: 2015-04-21 Impact factor: 9.079
Authors: Michelle C Sabo; Dara A Lehman; Bingjie Wang; Barbra A Richardson; Sujatha Srinivasan; Lusi Osborn; Daniel Matemo; John Kinuthia; Tina L Fiedler; Matthew M Munch; Alison L Drake; David N Fredricks; Julie Overbaugh; Grace John-Stewart; R Scott McClelland; Susan M Graham Journal: Sex Transm Infect Date: 2019-06-13 Impact factor: 3.519
Authors: Erin M Kahle; Michael Bolton; James P Hughes; Deborah Donnell; Connie Celum; Jairam R Lingappa; Allan Ronald; Craig R Cohen; Guy de Bruyn; Youyi Fong; Elly Katabira; M Juliana McElrath; Jared M Baeten Journal: J Infect Dis Date: 2014-11-10 Impact factor: 5.226
Authors: Nelly R Mugo; Renee Heffron; Deborah Donnell; Anna Wald; Edwin O Were; Helen Rees; Connie Celum; James N Kiarie; Craig R Cohen; Kayitesi Kayintekore; Jared M Baeten Journal: AIDS Date: 2011-09-24 Impact factor: 4.177
Authors: Eric Armstrong; Anke Hemmerling; Steve Miller; Kerianne E Burke; Sara J Newmann; Sheldon R Morris; Hilary Reno; Sanja Huibner; Maria Kulikova; Nico Nagelkerke; Bryan Coburn; Craig R Cohen; Rupert Kaul Journal: Lancet Microbe Date: 2022-04-21