Literature DB >> 32700445

PAX6D instructs neural retinal specification from human embryonic stem cell-derived neuroectoderm.

Yunlong Tao1, Jingyuan Cao1, Mingxing Li2, Brianna Hoffmann1, Ke Xu1, Jing Chen2, Xin Lu3, Fangliang Guo4, Xiang Li1, M Joseph Phillips1,5, David M Gamm1,5,6, Hong Chen2, Su-Chun Zhang1,7,8.   

Abstract

PAX6 is essential for neural retina (NR) and forebrain development but how PAX6 instructs NR versus forebrain specification remains unknown. We found that the paired-less PAX6, PAX6D, is expressed in NR cells during human eye development and along human embryonic stem cell (hESC) specification to retinal cells. hESCs deficient for PAX6D failed to enter NR specification. Induced expression of PAX6D but not PAX6A in a PAX6-null background restored the NR specification capacity. ChIP-Seq, confirmed by functional assays, revealed a set of retinal genes and non-retinal neural genes that are potential targets of PAX6D, including WNT8B. Inhibition of WNTs or knocking down of WNT8B restored the NR specification capacity of neuroepithelia with PAX6D knockout, whereas activation of WNTs blocked NR specification even when PAX6D was induced. Thus, PAX6D specifies neuroepithelia to NR cells via the regulation of WNT8B.
© 2020 The Authors.

Entities:  

Keywords:  zzm321990WNTzzm321990; PAX6 isoform; human pluripotent stem cell; neuroepithelia; retinal progenitor

Mesh：

Substances：

Year:  2020        PMID： 32700445      PMCID： PMC7507545          DOI： 10.15252/embr.202050000

Source DB:  PubMed          Journal:  EMBO Rep        ISSN： 1469-221X            Impact factor:   8.807


  78 in total

1.  Pax6 activity in the lens primordium is required for lens formation and for correct placement of a single retina in the eye.

Authors:  R Ashery-Padan; T Marquardt; X Zhou; P Gruss
Journal:  Genes Dev       Date:  2000-11-01       Impact factor: 11.361

2.  Pax6 expressed in osteocytes inhibits canonical Wnt signaling.

Authors:  Ajita Jami; Jogeswar Gadi; Min Jung Lee; Eun Jin Kim; Mi Jeong Lee; Han-Sung Jung; Hong-Hee Kim; Sung-Kil Lim
Journal:  Mol Cells       Date:  2013-03-22       Impact factor: 5.034

3.  Mouse small eye results from mutations in a paired-like homeobox-containing gene.

Authors:  R E Hill; J Favor; B L Hogan; C C Ton; G F Saunders; I M Hanson; J Prosser; T Jordan; N D Hastie; V van Heyningen
Journal:  Nature       Date:  1991 Dec 19-26       Impact factor: 49.962

4.  Two independent and interactive DNA-binding subdomains of the Pax6 paired domain are regulated by alternative splicing.

Authors:  J A Epstein; T Glaser; J Cai; L Jepeal; D S Walton; R L Maas
Journal:  Genes Dev       Date:  1994-09-01       Impact factor: 11.361

Review 5.  The role of Pax6 in forebrain development.

Authors:  Petrina A Georgala; Catherine B Carr; David J Price
Journal:  Dev Neurobiol       Date:  2011-08       Impact factor: 3.964

6.  Modeling early retinal development with human embryonic and induced pluripotent stem cells.

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Journal:  Proc Natl Acad Sci U S A       Date:  2009-08-25       Impact factor: 11.205

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Authors:  Hong Liu; Othman Mohamed; Daniel Dufort; Valerie A Wallace
Journal:  Dev Dyn       Date:  2003-07       Impact factor: 3.780

8.  Photoreceptor-specific expression, light-dependent localization, and transcriptional targets of the zinc-finger protein Yin Yang 1 in the chicken retina.

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10.  Efficient generation of region-specific forebrain neurons from human pluripotent stem cells under highly defined condition.

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Journal:  Sci Rep       Date:  2015-12-16       Impact factor: 4.379
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  2 in total

1.  PAX6D instructs neural retinal specification from human embryonic stem cell-derived neuroectoderm.

Authors:  Yunlong Tao; Jingyuan Cao; Mingxing Li; Brianna Hoffmann; Ke Xu; Jing Chen; Xin Lu; Fangliang Guo; Xiang Li; M Joseph Phillips; David M Gamm; Hong Chen; Su-Chun Zhang
Journal:  EMBO Rep       Date:  2020-07-23       Impact factor: 8.807

2.  LncRNA DUXAP10 Upregulation and the Hedgehog Pathway Activation Are Critically Involved in Chronic Cadmium Exposure-Induced Cancer Stem Cell-Like Property.

Authors:  Hsuan-Pei Lin; Zhishan Wang; Chengfeng Yang
Journal:  Toxicol Sci       Date:  2021-10-27       Impact factor: 4.849
  2 in total

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