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Literature DB >> 3266988

Rapid isoelectric focusing of plasmid-mediated beta-lactamases with Pharmacia PhastSystem.

Abstract

A modified isoelectric focusing method for rapid semiquantitative identification of plasmid-mediated beta-lactamases by use of the Pharmacia PhastSystem (Uppsala, Sweden) is described. Sonication of bacterial colonies collected directly from growth plates decreased the time required for the procedure. With sonic extracts of known beta-lactamase-producing strains used as controls, the assay could be completed in less than 2 h.

Mesh：

Substances：
DNA
beta-Lactamases

Year: 1988 PMID： 3266988 PMCID： PMC175959 DOI： 10.1128/AAC.32.11.1730

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

4 in total

1. Detection of plasmid-mediated beta-lactamases with DNA probes.

Authors: S Huovinen; P Huovinén; G A Jacoby
Journal: Antimicrob Agents Chemother Date: 1988-02 Impact factor: 5.191

2. The use of analytical isoelectric focusing for detection and identification of beta-lactamases.

Authors: A Mathew; A M Harris; M J Marshall; G W Ross
Journal: J Gen Microbiol Date: 1975-05

3. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.

Authors: C H O'Callaghan; A Morris; S M Kirby; A H Shingler
Journal: Antimicrob Agents Chemother Date: 1972-04 Impact factor: 5.191

4. Ampicillin resistance in Haemophilus influenzae: identification of resistance mechanisms.

Authors: A J Reid; I N Simpson; P B Harper; S G Amyes
Journal: J Antimicrob Chemother Date: 1987-11 Impact factor: 5.790

4 in total

15 in total

1. Epidemiology of extended-spectrum beta-lactamases in Sofia, Bulgaria.

Authors: G A Jacoby; R Vacheva-Dobrevsky
Journal: Eur J Clin Microbiol Infect Dis Date: 2003-05-29 Impact factor: 3.267

2. Dramatic increase in prevalence of fecal carriage of extended-spectrum beta-lactamase-producing Enterobacteriaceae during nonoutbreak situations in Spain.

Authors: Aránzazu Valverde; Teresa M Coque; M Paz Sánchez-Moreno; Azucena Rollán; Fernando Baquero; Rafael Cantón
Journal: J Clin Microbiol Date: 2004-10 Impact factor: 5.948

3. Spread of Escherichia coli strains with high-level cefotaxime and ceftazidime resistance between the community, long-term care facilities, and hospital institutions.

Authors: Jesús Oteo; Carmen Navarro; Emilia Cercenado; Alberto Delgado-Iribarren; Isabel Wilhelmi; Beatriz Orden; Carmen García; Silvia Miguelañez; María Pérez-Vázquez; Silvia García-Cobos; Belén Aracil; Verónica Bautista; José Campos
Journal: J Clin Microbiol Date: 2006-07 Impact factor: 5.948

4. qnrB, another plasmid-mediated gene for quinolone resistance.

Authors: George A Jacoby; Kelley E Walsh; Debra M Mills; Victoria J Walker; Herin Oh; Ari Robicsek; David C Hooper
Journal: Antimicrob Agents Chemother Date: 2006-04 Impact factor: 5.191

5. Gene sequence and biochemical characterization of FOX-1 from Klebsiella pneumoniae, a new AmpC-type plasmid-mediated beta-lactamase with two molecular variants.

Authors: M Gonzalez Leiza; J C Perez-Diaz; J Ayala; J M Casellas; J Martinez-Beltran; K Bush; F Baquero
Journal: Antimicrob Agents Chemother Date: 1994-09 Impact factor: 5.191

6. Analysis of beta-lactamase production in ampicillin-resistant Escherichia coli isolated from blood cultures 1983-1989.

Authors: T Arstila; S Huovinen; P Huovinen
Journal: Eur J Clin Microbiol Infect Dis Date: 1991-12 Impact factor: 3.267

7. Convergent in vivo and in vitro selection of ceftazidime resistance mutations at position 167 of CTX-M-3 beta-lactamase in hypermutable Escherichia coli strains.

Authors: Marina N Stepanova; Maxim Pimkin; Anatoly A Nikulin; Varvara K Kozyreva; Elena D Agapova; Mikhail V Edelstein
Journal: Antimicrob Agents Chemother Date: 2008-01-22 Impact factor: 5.191