Literature DB >> 326659

Culture of rat retinal pigment epithelium.

R B Edwards.   

Abstract

A method of preparing monolayer cultures of retinal pigment epithelium from normal pigmented neonatal rats is described. Critical features include incubating the eyes in balanced salt solution and treating with trypsin before dissecting the eyes. The tissue also has been culured from RCS rats with inherited retinal degeneration. Since the pigment epithelium has been shown to be the primary site of action of the gene for retinal dystrophy in the RCS rat, the method should be usefull in studying the defect(s) associated with this mutation.

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Year:  1977        PMID： 326659     DOI： 10.1007/bf02616175

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  14 in total

1.  An improved nutrient solution for diploid Chinese hamster and human cell lines.

Authors:  R G HAM
Journal:  Exp Cell Res       Date:  1963-02       Impact factor: 3.905

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Tissue culture of human retinal pigment epithelium.

Authors:  J Mannagh; D V Arya; A R Irvine
Journal:  Invest Ophthalmol       Date:  1973-01

4.  The retinal pigment epithelium. I. Comparative histology.

Authors:  M O Ts'o; E Friedman
Journal:  Arch Ophthalmol       Date:  1967-11

5.  Inherited retinal dystrophy: primary defect in pigment epithelium determined with experimental rat chimeras.

Authors:  R J Mullen; M M LaVail
Journal:  Science       Date:  1976-05-21       Impact factor: 47.728

6.  Accumulation of taurine by cultured retinal pigment epithelium of the rat.

Authors:  R B Edwards
Journal:  Invest Ophthalmol Vis Sci       Date:  1977-03       Impact factor: 4.799

7.  Participation of the retinal pigment epithelium in the rod outer segment renewal process.

Authors:  R W Young; D Bok
Journal:  J Cell Biol       Date:  1969-08       Impact factor: 10.539

8.  Photoreceptor-pigment epithelial cell relationships in rats with inherited retinal degeneration. Radioautographic and electron microscope evidence for a dual source of extra lamellar material.

Authors:  M M LaVail; R L Sidman; D O'Neil
Journal:  J Cell Biol       Date:  1972-04       Impact factor: 10.539

9.  The role of the pigment epithelium in the etiology of inherited retinal dystrophy in the rat.

Authors:  D Bok; M O Hall
Journal:  J Cell Biol       Date:  1971-06       Impact factor: 10.539

10.  Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects.

Authors:  T T PUCK; S J CIECIURA; A ROBINSON
Journal:  J Exp Med       Date:  1958-12-01       Impact factor: 14.307
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  10 in total

1.  Novel method for the rapid isolation of RPE cells specifically for RNA extraction and analysis.

Authors:  Cynthia Xin-Zhao Wang; Kaiyan Zhang; Bogale Aredo; Hua Lu; Rafael L Ufret-Vincenty
Journal:  Exp Eye Res       Date:  2012-06-18       Impact factor: 3.467

2.  Comparison of tight junction permeability for albumin in iris pigment epithelium and retinal pigment epithelium in vitro.

Authors:  K A Rezai; A Lappas; L Kohen; P Wiedemann; K Heimann
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1997-01       Impact factor: 3.117

3.  Short-term organ culture of the retinal pigment epithelium in microtitration plates: ultrastructural studies.

Authors:  N M McKechnie; W A Keegan; C A Converse; W S Foulds
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1986       Impact factor: 3.117

4.  Cultured retinal pigment epithelial cells from RCS rats express an increased calcium conductance compared with cells from non-dystrophic rats.

Authors:  O Strauss; M Wienrich
Journal:  Pflugers Arch       Date:  1993-10       Impact factor: 3.657

5.  Regulation of intracellular pH in cultured bovine retinal pigment epithelial cells.

Authors:  S K Keller; T J Jentsch; I Janicke; M Wiederholt
Journal:  Pflugers Arch       Date:  1988-01       Impact factor: 3.657

Review 6.  The cell biology of the retinal pigment epithelium.

Authors:  Aparna Lakkaraju; Ankita Umapathy; Li Xuan Tan; Lauren Daniele; Nancy J Philp; Kathleen Boesze-Battaglia; David S Williams
Journal:  Prog Retin Eye Res       Date:  2020-02-24       Impact factor: 19.704

7.  A Method for the Isolation and Culture of Adult Rat Retinal Pigment Epithelial (RPE) Cells to Study Retinal Diseases.

Authors:  Janosch P Heller; Jessica C F Kwok; Elena Vecino; Keith R Martin; James W Fawcett
Journal:  Front Cell Neurosci       Date:  2015-11-20       Impact factor: 5.505

Review 8.  Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations.

Authors:  Aaron H Fronk; Elizabeth Vargis
Journal:  J Tissue Eng       Date:  2016-07-12       Impact factor: 7.813

9.  Isolation and Culture of Primary Mouse Retinal Pigment Epithelial (RPE) Cells with Rho-Kinase and TGFβR-1/ALK5 Inhibitor.

Authors:  Junhui Shen; Jianfeng He; Fang Wang
Journal:  Med Sci Monit       Date:  2017-12-27

10.  The ternary Rab27a-Myrip-Myosin VIIa complex regulates melanosome motility in the retinal pigment epithelium.

Authors:  Vanda S Lopes; José S Ramalho; Dylan M Owen; Mike O Karl; Olaf Strauss; Clare E Futter; Miguel C Seabra
Journal:  Traffic       Date:  2007-05       Impact factor: 6.215
  10 in total

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