BACKGROUND AND PURPOSE: Spreading depolarizations (SDs) are recurrent and ostensibly spontaneous depolarization waves that may contribute to infarct progression after stroke. Somatosensory activation of the metastable peri-infarct tissue triggers peri-infarct SDs at a high rate. METHODS: We directly measured the functional activation threshold to trigger SDs in peri-infarct hot zones using optogenetic stimulation after distal middle cerebral artery occlusion in Thy1-ChR2-YFP mice. RESULTS: Optogenetic activation of peri-infarct tissue triggered SDs at a strikingly high rate (64%) compared with contralateral homotopic cortex (8%; P=0.004). Laser speckle perfusion imaging identified a residual blood flow of 31±2% of baseline marking the metastable tissue with a propensity to develop SDs. CONCLUSIONS: Our data reveal a spatially distinct increase in SD susceptibility in peri-infarct tissue where physiological levels of functional activation are capable of triggering SDs. Given the potentially deleterious effects of peri-infarct SDs, the effect of sensory overstimulation in hyperacute stroke should be examined more carefully.
BACKGROUND AND PURPOSE: Spreading depolarizations (SDs) are recurrent and ostensibly spontaneous depolarization waves that may contribute to infarct progression after stroke. Somatosensory activation of the metastable peri-infarct tissue triggers peri-infarct SDs at a high rate. METHODS: We directly measured the functional activation threshold to trigger SDs in peri-infarct hot zones using optogenetic stimulation after distal middle cerebral artery occlusion in Thy1-ChR2-YFP mice. RESULTS: Optogenetic activation of peri-infarct tissue triggered SDs at a strikingly high rate (64%) compared with contralateral homotopic cortex (8%; P=0.004). Laser speckle perfusion imaging identified a residual blood flow of 31±2% of baseline marking the metastable tissue with a propensity to develop SDs. CONCLUSIONS: Our data reveal a spatially distinct increase in SD susceptibility in peri-infarct tissue where physiological levels of functional activation are capable of triggering SDs. Given the potentially deleterious effects of peri-infarct SDs, the effect of sensory overstimulation in hyperacute stroke should be examined more carefully.
Authors: Maximilian Böhm; David Y Chung; Carlos A Gómez; Tao Qin; Tsubasa Takizawa; Homa Sadeghian; Kazutaka Sugimoto; Sava Sakadžić; Mohammad A Yaseen; Cenk Ayata Journal: J Cereb Blood Flow Metab Date: 2019-05-07 Impact factor: 6.200
Authors: David Y Chung; Kazutaka Sugimoto; Paul Fischer; Maximilian Böhm; Tsubasa Takizawa; Homa Sadeghian; Andreia Morais; Andrea Harriott; Fumiaki Oka; Tao Qin; Nils Henninger; Mohammad A Yaseen; Sava Sakadžić; Cenk Ayata Journal: J Neurosci Methods Date: 2018-09-05 Impact factor: 2.390
Authors: Cenk Ayata; Andrew K Dunn; Yasemin Gursoy-OZdemir; Zhihong Huang; David A Boas; Michael A Moskowitz Journal: J Cereb Blood Flow Metab Date: 2004-07 Impact factor: 6.200
Authors: H Wang; J Peca; M Matsuzaki; K Matsuzaki; J Noguchi; L Qiu; D Wang; F Zhang; E Boyden; K Deisseroth; H Kasai; W C Hall; G Feng; G J Augustine Journal: Proc Natl Acad Sci U S A Date: 2007-05-01 Impact factor: 11.205