| Literature DB >> 32640191 |
Xiao Liu1, Jean-Denis Beaudoin1, Carrie Ann Davison1, Sara G Kosmaczewski1, Benjamin I Meyer1, Antonio J Giraldez2, Marc Hammarlund3.
Abstract
The xbp-1 mRNA encodes the XBP-1 transcription factor, a critical part of the unfolded protein response. Here we report that an RNA fragment produced from xbp-1 mRNA cleavage is a biologically active non-coding RNA (ncRNA) essential for axon regeneration in Caenorhabditis elegans. We show that the xbp-1 ncRNA acts independently of the protein-coding function of the xbp-1 transcript as part of a dual output xbp-1 mRNA stress response axis. Structural analysis indicates that the function of the xbp-1 ncRNA depends on a single RNA stem; this stem forms only in the cleaved xbp-1 ncRNA fragment. Disruption of this stem abolishes the non-coding, but not the coding, function of the endogenous xbp-1 transcript. Thus, cleavage of the xbp-1 mRNA bifurcates it into a coding and a non-coding pathway; modulation of the two pathways may allow neurons to fine-tune their response to injury and other stresses.Entities:
Keywords: C. elegans; RNA processing; XBP1; axon regeneration; ncRNA; neuronal injury response; non-coding RNA; xbp-1
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Year: 2020 PMID： 32640191 PMCID： PMC7486263 DOI： 10.1016/j.neuron.2020.06.015
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 17.173