Marcel F Kunrath1, Marina S G Monteiro2, Saurabh Gupta3, Roberto Hubler4, Sílvia D de Oliveira2. 1. Pontifical Catholic University of Rio Grande Do Sul (PUCRS), School of Health and Life Sciences, Dentistry Department, Av. Ipiranga, P.O. Box 6681, 90619-900, Porto Alegre, RS, Brazil; Pontifical Catholic University of Rio Grande Do Sul (PUCRS), Materials and Nanoscience Laboratory, P.O. Box 1429, 90619-900, Porto Alegre, Brazil. Electronic address: marcelfkunrath@gmail.com. 2. Pontifical Catholic University of Rio Grande Do Sul (PUCRS), School of Health and Life Sciences, Immunology and Microbiology Laboratory, Av. Ipiranga, P.O. Box 6681, 90619-900, Porto Alegre, Brazil. 3. International Academy of Ceramic Implantology, Silver Spring, MD 20901, USA. 4. Pontifical Catholic University of Rio Grande Do Sul (PUCRS), Materials and Nanoscience Laboratory, P.O. Box 1429, 90619-900, Porto Alegre, Brazil.
Abstract
OBJECTIVE: Surface alterations have been employed to enhance the osseointegration process in biomedical implants. However, these modifications may influence bacterial adhesion in different ways. Therefore, this study developed five different surfaces and evaluated the Staphylococcus epidermidis growth in early (1 h) and late (24 h) contact. DESIGN: The Titanium (Ti) and Zirconia (Zr) surfaces were divided in five groups and characterized concerning your morphology, roughness, wettability and chemical surface composition. Then, were evaluated regarding bacterial adhesion and biofilm formation/thickness, viability and morphology. RESULTS: Different topographies were manufactured resulting in a variety of combinations of surface properties. High roughness showed significantly higher bacterial adhesion in 1 h, while high hydrophilicity revealed greater bacterial proliferation in 24 h. Morphological changes were not found visually, however the viability test showed some cell membrane damage in the Ti micro and nano groups. CONCLUSIONS: Finally, surface distinct properties influence the growth of S. epidermidis independent of the based-material. Furthermore, some surface properties require precautions for use in contaminated sites according to the increased adhesion of S. epidermidis presented when in contact.
OBJECTIVE: Surface alterations have been employed to enhance the osseointegration process in biomedical implants. However, these modifications may influence bacterial adhesion in different ways. Therefore, this study developed five different surfaces and evaluated the Staphylococcus epidermidis growth in early (1 h) and late (24 h) contact. DESIGN: The Titanium (Ti) and Zirconia (Zr) surfaces were divided in five groups and characterized concerning your morphology, roughness, wettability and chemical surface composition. Then, were evaluated regarding bacterial adhesion and biofilm formation/thickness, viability and morphology. RESULTS: Different topographies were manufactured resulting in a variety of combinations of surface properties. High roughness showed significantly higher bacterial adhesion in 1 h, while high hydrophilicity revealed greater bacterial proliferation in 24 h. Morphological changes were not found visually, however the viability test showed some cell membrane damage in the Ti micro and nano groups. CONCLUSIONS: Finally, surface distinct properties influence the growth of S. epidermidis independent of the based-material. Furthermore, some surface properties require precautions for use in contaminated sites according to the increased adhesion of S. epidermidis presented when in contact.
Authors: Adele Evans; Anthony J Slate; Millie Tobin; Stephen Lynch; Joels Wilson Nieuwenhuis; Joanna Verran; Peter Kelly; Kathryn A Whitehead Journal: Antibiotics (Basel) Date: 2022-04-21
Authors: Marcel F Kunrath; André L M Vargas; Patrícia Sesterheim; Eduardo R Teixeira; Roberto Hubler Journal: J R Soc Interface Date: 2020-09-30 Impact factor: 4.118
Authors: Astrid H Paulitsch-Fuchs; Benjamin Bödendorfer; Lukas Wolrab; Nicole Eck; Nigel P Dyer; Birgit Lohberger Journal: Front Cell Infect Microbiol Date: 2022-03-01 Impact factor: 5.293
Authors: Victor Manuel Villapun Puzas; Luke N Carter; Christian Schröder; Paula E Colavita; David A Hoey; Mark A Webber; Owen Addison; Duncan E T Shepherd; Moataz M Attallah; Liam M Grover; Sophie C Cox Journal: ACS Biomater Sci Eng Date: 2022-09-20