Satoru Kake1,2, Hiroaki Kawaguchi3, Tomoka Nagasato4,5, Tomonobu Yamada6, Takashi Ito4, Ikuro Maruyama4, Naoki Miura7, Akihide Tanimoto8. 1. Laboratory of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan. 2. Department of Comparative Animal Science, College of Life Science, Kurashiki University of Science and The Arts, Okayama, Japan. 3. Hygiene and Health Promotion Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. 4. Systems Biology in Thromboregulation, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. 5. Research Institute, Fujimori Kogyo Co., Ltd., Kanagawa, Japan. 6. Drug Safety Research Laboratories, Shin Nippon Biomedical Laboratories, Ltd., Kagoshima, Japan. 7. Veterinary Teaching Hospital, Joint faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan k9236024@kadai.jp akit09@m3.kufm.kagoshima-u.ac.jp. 8. Department of Molecular and Cellular Pathology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan k9236024@kadai.jp akit09@m3.kufm.kagoshima-u.ac.jp.
Abstract
BACKGROUND/AIM: An appropriate animal model is essential to investigate the relationship between inflammation, atherosclerosis, and thrombogenesis, and the development of preventive measures and therapies for atherosclerosis. MATERIALS AND METHODS: Atherosclerosis was induced in Microminipigs (MMPs) using a high-fat diet. We assessed high mobility group box 1 (HMGB1) expression levels and measured thrombus formation using a Total Thrombus Formation Analysis System (T-TAS). MMPs were divided into a normal diet (control) group and four high-fat diet groups, with differing amounts of cholesterol. After 8 weeks, blood was collected for analysis. RESULTS: HMGB1 levels increased with increasing dietary cholesterol, and a negative correlation was found between HMGB1 levels and thrombus formation time. CONCLUSION: T-TAS is useful in the assessment of thrombogenesis in MMPs and HMGB1 is associated with thrombus formation. Copyright
BACKGROUND/AIM: An appropriate animal model is essential to investigate the relationship between inflammation, atherosclerosis, and thrombogenesis, and the development of preventive measures and therapies for atherosclerosis. MATERIALS AND METHODS:Atherosclerosis was induced in Microminipigs (MMPs) using a high-fat diet. We assessed high mobility group box 1 (HMGB1) expression levels and measured thrombus formation using a Total Thrombus Formation Analysis System (T-TAS). MMPs were divided into a normal diet (control) group and four high-fat diet groups, with differing amounts of cholesterol. After 8 weeks, blood was collected for analysis. RESULTS:HMGB1 levels increased with increasing dietary cholesterol, and a negative correlation was found between HMGB1 levels and thrombus formation time. CONCLUSION:T-TAS is useful in the assessment of thrombogenesis in MMPs and HMGB1 is associated with thrombus formation. Copyright
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