Tomasz Szaflik1, Beata Smolarz2, Beata Mroczkowska1, Bartosz Kulig1, Malwina Soja1, Hanna Romanowicz3, Magdalena BryŚ4, Ewa Forma4, Krzysztof SzyŁŁo1. 1. Department of Operative Gynaecology and Oncological Gynaecology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland. 2. Laboratory of Cancer Genetics, Department of Pathology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland smolbea@wp.pl. 3. Laboratory of Cancer Genetics, Department of Pathology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland. 4. Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Abstract
AIM: The analysis of oestrogen receptor (ESR2) and cytochrome P450 family 19 subfamily A member (CYP19A1) gene expression in the context of the risk for endometriosis development. MATERIALS AND METHODS: Tissue specimens, collected from patients with endometriosis (n=100) and from control patients (n=100) embedded into paraffin blocks, provided the material for genetic studies, oriented towards the expression of ESR2 and CYP19A1 genes. The gene expression was assessed by the reverse transcription-polymerase chain reaction technique. RESULTS: Higher expression levels of ESR2 gene were demonstrated in the patients with endometriosis in comparison with the healthy controls. The expression intensity of CYP19A1 gene was associated with endometriosis, manifested as abdominal wall nodules. A relationship was observed between CYP19A1 gene expression and the Revised American Society for Reproductive Medicine classification in the group with ovarian endometrioid cysts, as well as in the group with peritoneal endometriosis. CONCLUSION: This study suggests the significant role of ESR2 and CYP19A1 gene expression in the pathogenesis of endometriosis. Copyright
AIM: The analysis of oestrogen receptor (ESR2) and cytochrome P450 family 19 subfamily A member (CYP19A1) gene expression in the context of the risk for endometriosis development. MATERIALS AND METHODS: Tissue specimens, collected from patients with endometriosis (n=100) and from control patients (n=100) embedded into paraffin blocks, provided the material for genetic studies, oriented towards the expression of ESR2 and CYP19A1 genes. The gene expression was assessed by the reverse transcription-polymerase chain reaction technique. RESULTS: Higher expression levels of ESR2 gene were demonstrated in the patients with endometriosis in comparison with the healthy controls. The expression intensity of CYP19A1 gene was associated with endometriosis, manifested as abdominal wall nodules. A relationship was observed between CYP19A1 gene expression and the Revised American Society for Reproductive Medicine classification in the group with ovarian endometrioid cysts, as well as in the group with peritoneal endometriosis. CONCLUSION: This study suggests the significant role of ESR2 and CYP19A1 gene expression in the pathogenesis of endometriosis. Copyright
Authors: Serdar E Bulun; Diana Monsavais; Mary Ellen Pavone; Matthew Dyson; Qing Xue; Erkut Attar; Hideki Tokunaga; Emily J Su Journal: Semin Reprod Med Date: 2012-01-23 Impact factor: 1.303
Authors: Katiane de Almeida Da Costa; Helena Malvezzi; Cristine Dobo; Rosa Maria Neme; Renée Zon Filippi; Thiago Pinheiro Arrais Aloia; Elisa Rampazo Prado; Juliana Meola; Carla de Azevedo Piccinato Journal: Front Mol Biosci Date: 2022-05-03