BACKGROUND & AIMS: Intestinal epithelial cells (IECs) provide a barrier that separates the mucosal immune system from the luminal microbiota. IECs constitutively express low levels of major histocompatibility complex (MHC) class II proteins, which are upregulated upon exposure to interferon gamma. We investigated the effects of deleting MHCII proteins specifically in mice with infectious, dextran sodium sulfate (DSS)-, and T-cell-induced colitis. METHODS: We disrupted the histocompatibility 2, class II antigen A, beta 1 gene (H2-Ab1) in IECs of C57BL/6 mice (I-AbΔIEC) or Rag1-/- mice (Rag1-/-I-AbΔIEC); we used I-AbWT mice as controls. Colitis was induced by administration of DSS, transfer of CD4+CD45RBhi T cells, or infection with Citrobacter rodentium. Colon tissues were collected and analyzed by histology, immunofluorescence, xMAP, and reverse-transcription polymerase chain reaction and organoids were generated. Microbiota (total and immunoglobulin [Ig]A-coated) in intestinal samples were analyzed by16S amplicon profiling. IgA+CD138+ plasma cells from Peyer's patches and lamina propria were analyzed by flow cytometry and IgA repertoire was determined by next-generation sequencing. RESULTS: Mice with IEC-specific loss of MHCII (I-AbΔIEC mice) developed less severe DSS- or T-cell transfer-induced colitis than control mice. Intestinal tissues from I-AbΔIEC mice had a lower proportion of IgA-coated bacteria compared with control mice, and a reduced luminal concentration of secretory IgA (SIgA) following infection with C rodentium. There was no significant difference in the mucosal IgA repertoire of I-AbΔIEC vs control mice, but opsonization of cultured C rodentium by SIgA isolated from I-AbΔIEC mice was 50% lower than that of SIgA from mAbWT mice. Fifty percent of I-AbΔIEC mice died after infection with C rodentium, compared with none of the control mice. We observed a transient but significant expansion of the pathogen in the feces of I-AbΔIEC mice compared with I-AbWT mice. CONCLUSIONS: In mice with DSS or T-cell-induced colitis, loss of MHCII from IECs reduces but does not eliminate mucosal inflammation. However, in mice with C rodentium-induced colitis, loss of MHCII reduces bacterial clearance by decreasing binding of IgA to commensal and pathogenic bacteria.
BACKGROUND & AIMS: Intestinal epithelial cells (IECs) provide a barrier that separates the mucosal immune system from the luminal microbiota. IECs constitutively express low levels of major histocompatibility complex (MHC) class II proteins, which are upregulated upon exposure to interferon gamma. We investigated the effects of deleting MHCII proteins specifically in mice with infectious, dextran sodium sulfate (DSS)-, and T-cell-induced colitis. METHODS: We disrupted the histocompatibility 2, class II antigen A, beta 1 gene (H2-Ab1) in IECs of C57BL/6 mice (I-AbΔIEC) or Rag1-/- mice (Rag1-/-I-AbΔIEC); we used I-AbWT mice as controls. Colitis was induced by administration of DSS, transfer of CD4+CD45RBhi T cells, or infection with Citrobacter rodentium. Colon tissues were collected and analyzed by histology, immunofluorescence, xMAP, and reverse-transcription polymerase chain reaction and organoids were generated. Microbiota (total and immunoglobulin [Ig]A-coated) in intestinal samples were analyzed by16S amplicon profiling. IgA+CD138+ plasma cells from Peyer's patches and lamina propria were analyzed by flow cytometry and IgA repertoire was determined by next-generation sequencing. RESULTS: Mice with IEC-specific loss of MHCII (I-AbΔIEC mice) developed less severe DSS- or T-cell transfer-induced colitis than control mice. Intestinal tissues from I-AbΔIEC mice had a lower proportion of IgA-coated bacteria compared with control mice, and a reduced luminal concentration of secretory IgA (SIgA) following infection with C rodentium. There was no significant difference in the mucosal IgA repertoire of I-AbΔIEC vs control mice, but opsonization of cultured C rodentium by SIgA isolated from I-AbΔIEC mice was 50% lower than that of SIgA from mAbWT mice. Fifty percent of I-AbΔIEC mice died after infection with C rodentium, compared with none of the control mice. We observed a transient but significant expansion of the pathogen in the feces of I-AbΔIEC mice compared with I-AbWT mice. CONCLUSIONS: In mice with DSS or T-cell-induced colitis, loss of MHCII from IECs reduces but does not eliminate mucosal inflammation. However, in mice with C rodentium-induced colitis, loss of MHCII reduces bacterial clearance by decreasing binding of IgA to commensal and pathogenic bacteria.
Authors: Moshe Biton; Adam L Haber; Noga Rogel; Grace Burgin; Semir Beyaz; Alexandra Schnell; Orr Ashenberg; Chien-Wen Su; Christopher Smillie; Karthik Shekhar; Zuojia Chen; Chuan Wu; Jose Ordovas-Montanes; David Alvarez; Rebecca H Herbst; Mei Zhang; Itay Tirosh; Danielle Dionne; Lan T Nguyen; Michael E Xifaras; Alex K Shalek; Ulrich H von Andrian; Daniel B Graham; Orit Rozenblatt-Rosen; Hai Ning Shi; Vijay Kuchroo; Omer H Yilmaz; Aviv Regev; Ramnik J Xavier Journal: Cell Date: 2018-11-01 Impact factor: 41.582
Authors: Tania K Uren; Odilia L C Wijburg; Cameron Simmons; Finn-Eirik Johansen; Per Brandtzaeg; Richard A Strugnell Journal: Eur J Immunol Date: 2005-01 Impact factor: 5.532
Authors: Frauke Borcherding; Martin Nitschke; Gheorghe Hundorfean; Jan Rupp; Dorthe von Smolinski; Katja Bieber; Cees van Kooten; Hendrik Lehnert; Klaus Fellermann; Jürgen Büning Journal: Am J Pathol Date: 2010-02-04 Impact factor: 4.307
Authors: Michael Bonelli; Anastasia Savitskaya; Carl-Walter Steiner; Eva Rath; Josef S Smolen; Clemens Scheinecker Journal: J Immunol Date: 2009-02-01 Impact factor: 5.422
Authors: Beth M Stadtmueller; Kathryn E Huey-Tubman; Carlos J López; Zhongyu Yang; Wayne L Hubbell; Pamela J Bjorkman Journal: Elife Date: 2016-03-04 Impact factor: 8.140
Authors: W Zac Stephens; Jason L Kubinak; Arevik Ghazaryan; Kaylyn M Bauer; Rickesha Bell; Kate Buhrke; Tyson R Chiaro; Allison M Weis; William W Tang; Josh K Monts; Ray Soto; H Atakan Ekiz; Ryan M O'Connell; June L Round Journal: Cell Rep Date: 2021-11-02 Impact factor: 9.995
Authors: L A Van Der Kraak; C Schneider; V Dang; A H P Burr; E S Weiss; J A Varghese; L Yang; T W Hand; S W Canna Journal: Mucosal Immunol Date: 2021-06-08 Impact factor: 7.313