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Literature DB >> 3258011

Transfected plasmacytoma cells do not transport the membrane form of IgM to the cell surface.

J Hombach¹, F Sablitzky, K Rajewsky, M Reth.

Abstract

Expression vectors coding for membrane-bound IgM antibodies were introduced into myeloma and B lymphoma cells. Only the lymphoma but not the myeloma cells were able to express the antibodies on the cell surface, although in both cases, complete antibodies were assembled intracellularly. In myeloma cells, the Ig molecules did not reach the Golgi compartment. Thus, the intracellular transport of membrane-bound antibodies is controlled in the B cell lineages in a developmentally ordered fashion.

Entities: Disease Species

Mesh：

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Year: 1988 PMID： 3258011 PMCID： PMC2188862 DOI： 10.1084/jem.167.2.652

Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307

18 in total

1. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.

Authors: J F Kearney; A Radbruch; B Liesegang; K Rajewsky
Journal: J Immunol Date: 1979-10 Impact factor: 5.422

2. High resolution two-dimensional electrophoresis of proteins.

Authors: P H O'Farrell
Journal: J Biol Chem Date: 1975-05-25 Impact factor: 5.157

3. Analysis of the repertoire of anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies in C 57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies.

Authors: M Reth; T Imanishi-Kari; K Rajewsky
Journal: Eur J Immunol Date: 1979-12 Impact factor: 5.532

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. Establishment and characterization of BALB/c lymphoma lines with B cell properties.

Authors: K J Kim; C Kanellopoulos-Langevin; R M Merwin; D H Sachs; R Asofsky
Journal: J Immunol Date: 1979-02 Impact factor: 5.422

6. The immunoglobulin mu chains of membrane-bound and secreted IgM molecules differ in their C-terminal segments.

Authors: M Kehry; S Ewald; R Douglas; C Sibley; W Raschke; D Fambrough; L Hood
Journal: Cell Date: 1980-09 Impact factor: 41.582

7. Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3' ends.

Authors: F W Alt; A L Bothwell; M Knapp; E Siden; E Mather; M Koshland; D Baltimore
Journal: Cell Date: 1980-06 Impact factor: 41.582

8. B lymphocyte differentiation and the control of IgM mu chain expression.

Authors: C Sidman
Journal: Cell Date: 1981-02 Impact factor: 41.582

9. Expression of a bacterial gene in mammalian cells.

Authors: R C Mulligan; P Berg
Journal: Science Date: 1980-09-19 Impact factor: 47.728

10. Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines.

Authors: F W Alt; N Rosenberg; V Enea; E Siden; D Baltimore
Journal: Mol Cell Biol Date: 1982-04 Impact factor: 4.272

12 in total

10. Dual role of the tyrosine activation motif of the Ig-alpha protein during signal transduction via the B cell antigen receptor.

Authors: H Flaswinkel; M Reth
Journal: EMBO J Date: 1994-01-01 Impact factor: 11.598

Transfected plasmacytoma cells do not transport the membrane form of IgM to the cell surface.

1. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.

2. High resolution two-dimensional electrophoresis of proteins.

3. Analysis of the repertoire of anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies in C 57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Establishment and characterization of BALB/c lymphoma lines with B cell properties.

6. The immunoglobulin mu chains of membrane-bound and secreted IgM molecules differ in their C-terminal segments.

7. Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3' ends.

8. B lymphocyte differentiation and the control of IgM mu chain expression.

9. Expression of a bacterial gene in mammalian cells.

10. Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines.

1. Heavy-chain only antibodies derived from dromedary are secreted and displayed by mouse B cells.

Review 2. Cellular signalling mechanisms in B lymphocytes.

3. Silencer elements controlling the B29 (Igbeta) promoter are neither promoter- nor cell-type-specific.

Review 4. Role of the BCR complex in B cell development, activation, and leukemic transformation.

5. The 'zinc knuckle' motif of Early B cell Factor is required for transcriptional activation of B cell-specific genes.

6. Molecular components of the B cell antigen receptor complex of class IgD differ partly from those of IgM.

7. A novel 34-kd protein co-isolated with the IgM molecule in surface IgM-expressing cells.

Review 8. Mechanisms controlling production of membrane and secreted immunoglobulin during B cell development.

9. Fate of surrogate light chains in B lineage cells.

10. Dual role of the tyrosine activation motif of the Ig-alpha protein during signal transduction via the B cell antigen receptor.