Literature DB >> 3257914

A dimeric inhibitor or insect alpha-amylase from barley. Cloning of the cDNA and identification of the protein.

A Lázaro1, R Sanchez-Monge, G Salcedo, J Paz-Ares, P Carbonero, F García-Olmedo.   

Abstract

A cDNA clone, designated pUP-44, whose longest open reading frame codes for a protein that is homologous to the wheat alpha-amylase inhibitors, has been isolated from a library obtained from developing barley endosperm. The deduced sequence for the mature protein, which is 122 residues long, is preceded by a sequence of 30 residues which has the typical features of a signal peptide. A closely corresponding protein, designated BDAI-1, has been isolated from mature endosperm. BDAI-1 behaves as a dimer and inhibits the alpha-amylase from the insect Tenebrio molitor at concentrations that have no effect on salivary or pancreatic alpha-amylases.

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Year:  1988        PMID： 3257914     DOI： 10.1111/j.1432-1033.1988.tb13864.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  10 in total

1.  A major barley allergen associated with baker's asthma disease is a glycosylated monomeric inhibitor of insect alpha-amylase: cDNA cloning and chromosomal location of the gene.

Authors:  M Mena; R Sanchez-Monge; L Gomez; G Salcedo; P Carbonero
Journal:  Plant Mol Biol       Date:  1992-11       Impact factor: 4.076

2.  Site-directed mutagenesis and expression in Escherichia coli of WMAI-1, a wheat monomeric inhibitor of insect alpha-amylase.

Authors:  F García-Maroto; P Carbonero; F García-Olmedo
Journal:  Plant Mol Biol       Date:  1991-11       Impact factor: 4.076

3.  Rye chromosome arm 3RS encodes a homodimeric inhibitor of insect α-amylase.

Authors:  G García-Casado; R Sánchez-Monge; C Lopez-Otín; G Salcedo
Journal:  Theor Appl Genet       Date:  1994-09       Impact factor: 5.699

4.  Cloning of cDNA and chromosomal location of genes encoding the three types of subunits of the wheat tetrameric inhibitor of insect alpha-amylase.

Authors:  F García-Maroto; C Maraña; M Mena; F García-Olmedo; P Carbonero
Journal:  Plant Mol Biol       Date:  1990-05       Impact factor: 4.076

5.  Cloning and characterization of a cDNA encoding the wheat (Triticum durum Desf.) CM16 protein.

Authors:  M F Gautier; R Alary; P Joudrier
Journal:  Plant Mol Biol       Date:  1990-03       Impact factor: 4.076

6.  Characterization of LeMir, a root-knot nematode-induced gene in tomato with an encoded product secreted from the root.

Authors:  E D Brenner; K N Lambert; I Kaloshian; V M Williamson
Journal:  Plant Physiol       Date:  1998-09       Impact factor: 8.340

7.  Wheat Inhibitors of Heterologous alpha-Amylases : Characterization of Major Components from the Monomeric Class.

Authors:  L Gomez; R Sanchez-Monge; C Lopez-Otín; G Salcedo
Journal:  Plant Physiol       Date:  1991-07       Impact factor: 8.340

8.  Alpha-amylase inhibitor changes during processing of sweet potato and taro tubers.

Authors:  M R Rekha; G Padmaja
Journal:  Plant Foods Hum Nutr       Date:  2002       Impact factor: 3.921

9.  The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.).

Authors:  P Rodriguez-Palenzuela; J Royo; L Gómez; R Sánchez-Monge; G Salcedo; J L Molina-Cano; F Garcia-Olmedo; P Carbonero
Journal:  Mol Gen Genet       Date:  1989-11

10.  Cloning and sequencing of the cDNA encoding the major albumin of Theobroma cacao : Identification of the protein as a member of the Kunitz protease inhibitor family.

Authors:  M E Spencer; R Hodge
Journal:  Planta       Date:  1991-03       Impact factor: 4.116
  10 in total

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