Literature DB >> 2102817

Cloning and characterization of a cDNA encoding the wheat (Triticum durum Desf.) CM16 protein.

M F Gautier1, R Alary, P Joudrier.   

Abstract

A Triticum durum cDNA library prepared from developing endosperm (22 days after flowering (DAF] was screened using synthetic oligonucleotide probes covering part of the CM3 and CM16 N-terminal protein sequences. A full-length cDNA clone (pTd78) encoding the CM16 protein (chloroform/methanol-soluble protein) was isolated and characterized. To our knowledge this is the first characterization of a clone coding for a wheat CM protein. The CM16 protein is synthesized as a preprotein with a signal peptide of 24 residues, the molecular weight of the mature protein being 13,438 Da. As other members of the cereal trypsin/alpha-amylase inhibitor family, the CM16 protein contains 10 cysteine residues, their position being well conserved. In developing endosperm the highest level of CM16 mRNA was detected at mid-maturation.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2102817     DOI： 10.1007/bf00028768

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  25 in total

1.  Localization to chromosomes of structural genes for the major protease inhibitors of barley grains.

Authors:  J Hejgaard; S E Bjørn; G Nielsen
Journal:  Theor Appl Genet       Date:  1984-05       Impact factor: 5.699

2.  Chromosomal control of non-gliadin proteins from the 70% ethanol extract of wheat endosperm.

Authors:  C Aragoncillo; M A Rodriguez Loperena; P Carbonero; F Garcia-Olmedo
Journal:  Theor Appl Genet       Date:  1975-01       Impact factor: 5.699

3.  Molecular cloning of the barley seed protein CMd: a variant member of the alpha-amylase/trypsin inhibitor family of cereals.

Authors:  N G Halford; N A Morris; P Urwin; M S Williamson; D D Kasarda; E J Lew; M Kreis; P R Shewry
Journal:  Biochim Biophys Acta       Date:  1988-09-07

4.  A dimeric inhibitor or insect alpha-amylase from barley. Cloning of the cDNA and identification of the protein.

Authors:  A Lázaro; R Sanchez-Monge; G Salcedo; J Paz-Ares; P Carbonero; F García-Olmedo
Journal:  Eur J Biochem       Date:  1988-02-15

5.  A new method for predicting signal sequence cleavage sites.

Authors:  G von Heijne
Journal:  Nucleic Acids Res       Date:  1986-06-11       Impact factor: 16.971

6.  A simple and very efficient method for generating cDNA libraries.

Authors:  U Gubler; B J Hoffman
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

7.  Genetics of CM-proteins (A-hordeins) in barley.

Authors:  G Salcedo; P Fra-Mon; J L Molina-Cano; C Aragoncillo; F García-Olmedo
Journal:  Theor Appl Genet       Date:  1984-05       Impact factor: 5.699

8.  Wheat tetrameric inhibitors of insect alpha-amylases: Alloploid heterosis at the molecular level.

Authors:  L Gomez; R Sanchez-Monge; F Garcia-Olmedo; G Salcedo
Journal:  Proc Natl Acad Sci U S A       Date:  1989-05       Impact factor: 11.205

9.  New alpha-amylase and trypsin inhibitors among the CM-proteins of barley (Hordeum vulgare).

Authors:  D Barber; R Sanchez-Monge; E Mendez; A Lazaro; F Garcia-Olmedo; G Salcedo
Journal:  Biochim Biophys Acta       Date:  1986-01-17

10.  Studies on transformation of Escherichia coli with plasmids.

Authors:  D Hanahan
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469
View more
  10 in total

1.  Nucleotide sequence of a cDNA encoding a lipid transfer protein from wheat (Triticum durum Desf.).

Authors:  W Dieryck; M F Gautier; V Lullien; P Joudrier
Journal:  Plant Mol Biol       Date:  1992-07       Impact factor: 4.076

2.  Nucleotide sequence of a cDNA clone encoding the wheat (Triticum durum Desf.) CM2 protein.

Authors:  M F Gautier; R Alary; V Lullien; P Joudrier
Journal:  Plant Mol Biol       Date:  1991-02       Impact factor: 4.076

3.  Characterization of a cDNA clone encoding the Triticum aestivum L. CM16 protein: homology with the Triticum durum Desf. sequence.

Authors:  V Lullien; R Alary; P Joudrier; M F Gautier
Journal:  Plant Mol Biol       Date:  1991-02       Impact factor: 4.076

4.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

5.  WCI, a novel wheat chymotrypsin inhibitor: purification, primary structure, inhibitory properties and heterologous expression.

Authors:  Antimo Di Maro; Francesca Farisei; Daniela Panichi; Valeria Severino; Natalia Bruni; Anna Grazia Ficca; Pasquale Ferranti; Valeria Capuzzi; Francesca Tedeschi; Elia Poerio
Journal:  Planta       Date:  2011-05-27       Impact factor: 4.116

6.  Isolation and nucleotide sequence of a cDNA clone encoding the bread wheat (Triticum aestivum L.) CM17 protein.

Authors:  V Lullien; R Alary; A Guirao; P Joudrier; M F Gautier
Journal:  Plant Mol Biol       Date:  1991-11       Impact factor: 4.076

7.  Triticum aestivum puroindolines, two basic cystine-rich seed proteins: cDNA sequence analysis and developmental gene expression.

Authors:  M F Gautier; M E Aleman; A Guirao; D Marion; P Joudrier
Journal:  Plant Mol Biol       Date:  1994-04       Impact factor: 4.076

8.  Insight into the molecular evolution of non-specific lipid transfer proteins via comparative analysis between rice and sorghum.

Authors:  Hong Wei Wang; Sun-Goo Hwang; Thirupathi Karuppanapandian; Aihua Liu; Wook Kim; Cheol Seong Jang
Journal:  DNA Res       Date:  2012-02-24       Impact factor: 4.458

9.  The spectrum of low molecular weight alpha-amylase/protease inhibitor genes expressed in the US bread wheat cultivar Butte 86.

Authors:  Susan B Altenbach; William H Vensel; Frances M Dupont
Journal:  BMC Res Notes       Date:  2011-07-20

10.  Sina and Sinb genes in triticale do not determine grain hardness contrary to their orthologs Pina and Pinb in wheat.

Authors:  Sebastian Gasparis; Waclaw Orczyk; Anna Nadolska-Orczyk
Journal:  BMC Plant Biol       Date:  2013-11-26       Impact factor: 4.215
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.