| Literature DB >> 32567019 |
Jakob Jeriha1, Nikola Kolundzic2, Preeti Khurana2, Andrea Perez-Dominguez2, Dusko Ilic2.
Abstract
Reprogramming somatic cells into induced pluripotent stem cells (iPSC) has provided a gateway for many novel discoveries in the field of tissue engineering, regenerative medicine and cell therapy. The need for an efficient, less laborious and fast reprogramming protocol under xeno-free, feeder-free and chemically defined conditions has never been greater. Here we describe a novel approach to reprogramming using the StemRNA 3rd Gen Reprogramming Kit (ReproCELL) which encompasses non-modified microRNAs (NM-miRNA), non-modified E3, K3, B18R RNAs (EKB NM-RNA) and non-modified mRNAs for six crucial transcription factors (OSKMNL NM-RNA).Entities:
Keywords: Chemically defined; Feeder-free; Reprogramming; Xeno-free; iPSC; mRNA; miRNA
Mesh:
Substances:
Year: 2022 PMID: 32567019 DOI: 10.1007/7651_2020_302
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745